Enzyme
In addition to the desaturation of (2R,3R)-dihydroflavonols to flavonols, the enzyme from Citrus unshiu (satsuma mandarin) also has a non-specific activity that trans-hydroxylates the flavanones (2S)-naringenin and the unnatural (2R)-naringenin at C-3 to kaempferol and (2R,3R)-dihydrokaempferol, respectively [2]. Requires Fe2+.
FLS
Enzyme
Flavonol synthase
Enzyme name
EC:1.14.20.6
Entry
Oxidoreductases;Acting on paired donors, with incorporation or reduction of molecular oxygen;With 2-oxoglutarate as one donor, and the other dehydrogenated
Class
dihydroflavonol,2-oxoglutarate:oxygen oxidoreductase
Sysname
a dihydroflavonol + 2-oxoglutarate + O2 = a flavonol + succinate + CO2 + H2O [RN:R07368]
Reaction
flavonol [CPD:C01495];succinate [CPD:C00042];CO2 [CPD:C00011];H2O [CPD:C00001]
Product
Species information
Myagrum perfoliatum
Species name
Myagrum perfoliatum is a species of eudicot in the family Brassicaceae (mustard family).
Species info
Gene sequence info
Myagrum_perfoliatum.FLS.fa
Download
>Myper.0001s2271.1.v2.1
MKVNYYPPCPQPNLVLGLHPHTDIHGLTVLVPNVIPGLQVFKDDQWFDVQYNPSAIVFLIGDPIMRMSNGKYKNVLHRAKLNKEKTGMSWVVLLKPTYDMVIGPLTRLTGDGDETPKFKHITYGEHVYRKVNNLPLD
>Myper.0001s1396.1.v2.1
MELPVVDLSRYLQFSGGESDSHLETDLLDSCREVSRILRETGALLVKDPRCSAEDNDRFIDMMETYFSMPDDFKRLQERPNLHYQVGVTPEGVEVPRSLVDEEMQEKFKTMPKEYKPHTPKGPDHKWRYMWRVGPRPSNTRFKELNSEPVIPEGFPEWKEVMDSWGYKMISAVEVVAEMAAIGFGLPKDAFTSLMKQGPHLLAPTGSDLSCYNEEGTIFAGYHYDLNFLTIHGRSRFPGLYIWLRNGEKVAVKVPVGCLLIQTGKQIEWLTAGECIAGMHEVVVTSKTMDAIKLAKEQNRSLWRVSSTLFAHIASDAKLKPLGHFAESSLASNYPEIPAGEYVEQELSVINLKGKKTFS
>Myper.0001s2270.1.v2.1
MAESIPSELKVTAPKGIPVLDLSIPDEDLVARAVVKASEEWGIFQVVNHGIPAELIRRLKEVGTEFFELPETEKEVVARPADSTDLEGYTTDYKKDGEGRKTWADHLFHRIWPPSRINYRFWPKNSPDYREVNEEYAREIKKLSERILGWLSEGLGLRRDALKEGLGGETVEYLMKVIFYPPCPKLDLLYGAPHHTDLNGVTFLIADEVDGLQAFQDNNGSTSSMTTPGSSSSSPIKSRG
>Myper.0001s2267.1.v2.1
MEVERDQETSPPLEKIPIVDLSNPDEELVSRAVLKASKEWGIFHVVNHGISTDLILRLKEVGTQFFELPETEKKSVAKPDNSNDFEGYTRNLKYTEGEVWADNLFHRIWPPSCINYNYWPKNPPQYREVIEEYTEETKKLSERLLGVLSEGLGLRHEALKEGLGGETTEYVMRINNFPPNPQPDATLGLPEHTDIVAIALIVTNEVPGLQVFKDDHWFHVQYIPSAITITIGDQIMRLSNGKYKNVLHRVTVDKEKQRMSWPIFVDANPDVVIGPLPELITGDNPSMFKSFVCKDFKYRRLFKLPVD
>Myper.0001s2272.1.v2.1
MEAKRDQHTSPSSETIPTVDLSDPEEELVARAVAKASEEWGAFQVVNHGIPPELMRRLQEVGRQFFELPAAEKEAVAKLPDSTKLEGYDAKNLKRLESWNDHLLHKIWPHSCVDYRFWPKNPPNYREVNEEYARHVAKLSETIMGCLSKGLGLGREALKEGLGGGVYTLRINYYPPSDLVIGAPAHTDFAALALLVSNDVPGLQVFKDNHWVDVENIDSAVVVLVGDQIMRMSNGRYKSVLHRSTMEKEKTRMTWPVLVDPRPGLLVGPLPELIGDENPPKFESLTFKDYIYRKLNMLLRDG
>Myper.0001s2268.1.v2.1
MARNQHISLSSSLAETVPSKKEKPFITFQGTAMRISWPVFVESNLDHEFGPLMELITGDHINAPKFKPYVYKDFKFRKLKKLPLD
>Myper.0001s2269.1.v2.1
MEIERNQNISPSSLLAETIPAADEIPVIDLSNPDEGLVARAVVKASEEWGIFHVANHGIPPELIRRLKEVGREFYELPETEKKAVAKPEDSTDLEGYATNFEGWADHLFHRIWPPSRINYRFWPKNSPDYREVTEEYAKHIKKLSERIMEWLSEGLGLSRDALKEGLGGEKVEYLMKLIFYPPCQDFDMNDGAPPHTDFNGMTLLIANEVHGLEAFKDNKWIDVKYDDSMILVIIADEFHRMSNGKYKSAEHRATMDTEKTRISWPVFVEPSLDQVVGPLPGIISGDDNAPKFKPHAYRDFKFNLRNGLPLD
>Myper.0002s0329.1.v2.1
MENHTTMKVSSLNCIDLANDDLHHSVVSLKQACLDCGFFYVINHGINEEFMEDVFEQSKKFFDLPLEEKMKVLRNEKHRGYTPVLDEILDPQNQVNGDHKEGYYIGIEVPKDDPDWDKPFYGPNPWPESDVLPRWRETMEKYHQEALRVSKDIARLLALALDLDVNYFDTMEMLGKPIATMRLLHYQGISDPSKGIYACGAHSDYGMMTLLATDGVMGLQICKDKNDKPQKWEYVPPIKGAFIVNLGDMLERWSNGFFKSTLHRVLGNGQERYSIPFFVEPNHDCLVECLPTCKSESNVPKFSAIKCSTYLTQRYKETHAELSIYNQ
>Myper.0004s1006.1.v2.1
MEIERVQDISSSSLLTEAIPLEFIRSEKEQPAITTFRGPTPAIPVVDLSEPDEESVARAVVKASEEWGIFQVVNHGIPTELIQRLQEVGRTFFELPSAEKESVAKPADSKDIEGYGTKLQKDPEDKKAWVDHLFHRIWPPSCVNYRFWPKNPPQYREVNEEYAVHVKKLSETLLGILSEGLGLRSEALKEGLGGDMAEYMMKINYYPPCPRPDLALGVPAHTDLSGITLLVPNEVPGLQVFKDDHWFDAEYIPSAVIVHIGDQILRLSNGRYKNVLHRTTVDKEKTRMSWPVFLEPHREMVVGPLPELIRDDSPPKYKPFAFKDYSYRKLNKLPLD
>Myper.0005s1785.1.v2.1
METKSQEEDSIIQESLLTCIDLANYDLHQSALLLKQACLDCGFFYVMNHGISEEVMDKAFEQSKKFFALPLEEKMKVLKNEKHRGYTPLYDQIPDPENQVHGDHKEGYYIGAEVPRDDPQWDQPFYGPNPWPDPDLLPGWRETMEKYHQEALRVCKATAKLLALALDLDADYFDSPEMLGKPISTLRLLHYEGKSDPSKGIYGTGAHSDYGMMTLLATDGVLGLQICKDRNVKPQKWEYVPSIKGAIVVNLGDMMERWSNCLFKSTLHRVLLNGRNRYSIPFFVEPNHDCIIECLSTCQSENNLPKYPAIKCSKYLTQRYEESHANFSSYEKQA
>Myper.0005s1896.1.v2.1
MVLERDNDTPSTQVPVVDLSNPDQELVARAVVKASEEWGVFQVVNHGIPTELIQRLQNVGRRFFELSEPEKEAVARVGDSREGYARRYTLDLEKRIGTVDQLYHNLWPPSSVNYSYWPKNPQDYREVNEEYTRQVKKLSEKLMEWLSEGLGLRREAIKEAVDGEYLMNINYYPPCPHPDLIEGLDAHTDVSGLTLLLTNEIPGLQVFKDDQWIHVDYIPFAVIINISDQILRVSNGRYKSVLHKTTVDKERTRMSWAALVRPTYDMIVGPFPELISHDNPPKFKPMAYKDYIYRKIRNLPFFDLDS
>Myper.0005s1787.1.v2.1
METKSQEEDSIIQESLLTCIDLANYDLHQSALLLKQACLDCGFFYVMNHGISEEVMDKAFEQSKKFFALPLEEKMKVLKNEKHRGYTPLYDQIPDPENQVHGDHKEGYYIGAEVPRDDPQWDQPFYGPNPWPDPDLLPGWRETMEKYHQEALRVCKATAKLLALALDLDADYFDSPEMLGKPISTLRLLHYEGKSDPSKGIYGTGAHSDYGMMTLLATDGVLGLQICKDRNVKPQKWEYVPSIKGAIVVNLGDMMERWSNCLFKSTLHRVLLNGRNRYSIPFFVEPNHDCIIECLSTCQSENNLPKYPAIKCSKYLTQRYEESHANFSSYEKQA
>Myper.0005s1784.1.v2.1
METKSQEEDSIIQESLLTCIDLANYDLHQSALLLKQACLDCGFFYVMNHGISEEVMDKAFEQSKKFFALPLEEKMKVLKNEKHRGYTPLYDQIPDPENQVHGDHKEGYYIGAEVPRDDPQWDQPFYGPNPWPDPDLLPGWRETMEKYHQEALRVCKATAKLLALALDLDADYFDSPEMLGKPISTLRLLHYEGKSDPSKGIYGTGAHSDYGMMTLLATDGVLGLQICKDRNVKPQKWEYVPSIKGAIVVNLGDMMERWSNCLFKSTLHRVLLNGRNRYSIPFFVEPNHDCIIECLSTCQSENNLPKYPAIKCSKYLTQRYEESHANFSSYEKQA
>Myper.0005s1786.1.v2.1
METKSQEEDSIIQESLLTCIDLANYDLHQSALLLKQACLDCGFFYVMNHGISEEVMDKAFEQSKKFFALPLEEKMKVLKNEKHRGYTPLYDQIPDPENQVHGDHKEGYYIGAEVPRDDPQWDQPFYGPNPWPDPDLLPGWRETMEKYHQEALRVCKATAKLLALALDLDADYFDSPEMLGKPISTLRLLHYEGKSDPSKGIYGTGAHSDYGMMTLLATDGVLGLQICKDRNVKPQKWEYVPSIKGAIVVNLGDMMERWSNCLFKSTLHRVLLNGRNRYSIPFFVEPNHDCIIECLSTCQSENNLPKYPAIKCSKYLTQRYEESHANFSSYEKQA
>Myper.0008s0598.1.v2.1
MAETIPSEFQGTASEGIPVVDLSSDEDLVARAVVKASQEWGIFQVVKHGIPTELIRRLKEVGTEFFELPETEKELVARPADSTDFEGYTTDYKKDGKGRRTWADHLFHRVWPPSRINYRFWPENSPDYRKVNEEYAREIKKLSEKIMGWLSEGLGLSREALKEGLGGETVEYLMKVIFYPPCPDLDLLYGAPHHTDLNGITFLIANQVHGLQAFQDNKWIDVNYDDSGIVVIIADQIKRMSNGRYKCGEHRATMDTDRTRISWPVFAEPSLDHVVGPLPELIIGDDNAPKFKPYVYREYKFRKMNELPLD
>Myper.0008s0599.1.v2.1
MAETIPSGFQGTASEGIPVVDLSNSDEDLVARAVVKASQEWGIFLVVNHGIPTELIRRLKEVGTDFFELPETEKELIARPADSTDLEGYTTGYKKDEEGRKTWVDHFFHRVWPPSRINYRFWLENSPDYREVNEEYAREIKKLSEKIMGWLSEGLGLHREALKEGFGGETVEYLMKVIFYPPCPDLDLLYGTPHHTDLGVMTTLVANQVHGLQALQDNKWIDVNYDDSKIVVIIADQIKRMSNGRYQCGEHRAMMDKDRTRISWPVFVEPNLDHVVGPLPELIIGDDSAPKFKPYAYSEYKFRKLNELPLD
MKVNYYPPCPQPNLVLGLHPHTDIHGLTVLVPNVIPGLQVFKDDQWFDVQYNPSAIVFLIGDPIMRMSNGKYKNVLHRAKLNKEKTGMSWVVLLKPTYDMVIGPLTRLTGDGDETPKFKHITYGEHVYRKVNNLPLD
>Myper.0001s1396.1.v2.1
MELPVVDLSRYLQFSGGESDSHLETDLLDSCREVSRILRETGALLVKDPRCSAEDNDRFIDMMETYFSMPDDFKRLQERPNLHYQVGVTPEGVEVPRSLVDEEMQEKFKTMPKEYKPHTPKGPDHKWRYMWRVGPRPSNTRFKELNSEPVIPEGFPEWKEVMDSWGYKMISAVEVVAEMAAIGFGLPKDAFTSLMKQGPHLLAPTGSDLSCYNEEGTIFAGYHYDLNFLTIHGRSRFPGLYIWLRNGEKVAVKVPVGCLLIQTGKQIEWLTAGECIAGMHEVVVTSKTMDAIKLAKEQNRSLWRVSSTLFAHIASDAKLKPLGHFAESSLASNYPEIPAGEYVEQELSVINLKGKKTFS
>Myper.0001s2270.1.v2.1
MAESIPSELKVTAPKGIPVLDLSIPDEDLVARAVVKASEEWGIFQVVNHGIPAELIRRLKEVGTEFFELPETEKEVVARPADSTDLEGYTTDYKKDGEGRKTWADHLFHRIWPPSRINYRFWPKNSPDYREVNEEYAREIKKLSERILGWLSEGLGLRRDALKEGLGGETVEYLMKVIFYPPCPKLDLLYGAPHHTDLNGVTFLIADEVDGLQAFQDNNGSTSSMTTPGSSSSSPIKSRG
>Myper.0001s2267.1.v2.1
MEVERDQETSPPLEKIPIVDLSNPDEELVSRAVLKASKEWGIFHVVNHGISTDLILRLKEVGTQFFELPETEKKSVAKPDNSNDFEGYTRNLKYTEGEVWADNLFHRIWPPSCINYNYWPKNPPQYREVIEEYTEETKKLSERLLGVLSEGLGLRHEALKEGLGGETTEYVMRINNFPPNPQPDATLGLPEHTDIVAIALIVTNEVPGLQVFKDDHWFHVQYIPSAITITIGDQIMRLSNGKYKNVLHRVTVDKEKQRMSWPIFVDANPDVVIGPLPELITGDNPSMFKSFVCKDFKYRRLFKLPVD
>Myper.0001s2272.1.v2.1
MEAKRDQHTSPSSETIPTVDLSDPEEELVARAVAKASEEWGAFQVVNHGIPPELMRRLQEVGRQFFELPAAEKEAVAKLPDSTKLEGYDAKNLKRLESWNDHLLHKIWPHSCVDYRFWPKNPPNYREVNEEYARHVAKLSETIMGCLSKGLGLGREALKEGLGGGVYTLRINYYPPSDLVIGAPAHTDFAALALLVSNDVPGLQVFKDNHWVDVENIDSAVVVLVGDQIMRMSNGRYKSVLHRSTMEKEKTRMTWPVLVDPRPGLLVGPLPELIGDENPPKFESLTFKDYIYRKLNMLLRDG
>Myper.0001s2268.1.v2.1
MARNQHISLSSSLAETVPSKKEKPFITFQGTAMRISWPVFVESNLDHEFGPLMELITGDHINAPKFKPYVYKDFKFRKLKKLPLD
>Myper.0001s2269.1.v2.1
MEIERNQNISPSSLLAETIPAADEIPVIDLSNPDEGLVARAVVKASEEWGIFHVANHGIPPELIRRLKEVGREFYELPETEKKAVAKPEDSTDLEGYATNFEGWADHLFHRIWPPSRINYRFWPKNSPDYREVTEEYAKHIKKLSERIMEWLSEGLGLSRDALKEGLGGEKVEYLMKLIFYPPCQDFDMNDGAPPHTDFNGMTLLIANEVHGLEAFKDNKWIDVKYDDSMILVIIADEFHRMSNGKYKSAEHRATMDTEKTRISWPVFVEPSLDQVVGPLPGIISGDDNAPKFKPHAYRDFKFNLRNGLPLD
>Myper.0002s0329.1.v2.1
MENHTTMKVSSLNCIDLANDDLHHSVVSLKQACLDCGFFYVINHGINEEFMEDVFEQSKKFFDLPLEEKMKVLRNEKHRGYTPVLDEILDPQNQVNGDHKEGYYIGIEVPKDDPDWDKPFYGPNPWPESDVLPRWRETMEKYHQEALRVSKDIARLLALALDLDVNYFDTMEMLGKPIATMRLLHYQGISDPSKGIYACGAHSDYGMMTLLATDGVMGLQICKDKNDKPQKWEYVPPIKGAFIVNLGDMLERWSNGFFKSTLHRVLGNGQERYSIPFFVEPNHDCLVECLPTCKSESNVPKFSAIKCSTYLTQRYKETHAELSIYNQ
>Myper.0004s1006.1.v2.1
MEIERVQDISSSSLLTEAIPLEFIRSEKEQPAITTFRGPTPAIPVVDLSEPDEESVARAVVKASEEWGIFQVVNHGIPTELIQRLQEVGRTFFELPSAEKESVAKPADSKDIEGYGTKLQKDPEDKKAWVDHLFHRIWPPSCVNYRFWPKNPPQYREVNEEYAVHVKKLSETLLGILSEGLGLRSEALKEGLGGDMAEYMMKINYYPPCPRPDLALGVPAHTDLSGITLLVPNEVPGLQVFKDDHWFDAEYIPSAVIVHIGDQILRLSNGRYKNVLHRTTVDKEKTRMSWPVFLEPHREMVVGPLPELIRDDSPPKYKPFAFKDYSYRKLNKLPLD
>Myper.0005s1785.1.v2.1
METKSQEEDSIIQESLLTCIDLANYDLHQSALLLKQACLDCGFFYVMNHGISEEVMDKAFEQSKKFFALPLEEKMKVLKNEKHRGYTPLYDQIPDPENQVHGDHKEGYYIGAEVPRDDPQWDQPFYGPNPWPDPDLLPGWRETMEKYHQEALRVCKATAKLLALALDLDADYFDSPEMLGKPISTLRLLHYEGKSDPSKGIYGTGAHSDYGMMTLLATDGVLGLQICKDRNVKPQKWEYVPSIKGAIVVNLGDMMERWSNCLFKSTLHRVLLNGRNRYSIPFFVEPNHDCIIECLSTCQSENNLPKYPAIKCSKYLTQRYEESHANFSSYEKQA
>Myper.0005s1896.1.v2.1
MVLERDNDTPSTQVPVVDLSNPDQELVARAVVKASEEWGVFQVVNHGIPTELIQRLQNVGRRFFELSEPEKEAVARVGDSREGYARRYTLDLEKRIGTVDQLYHNLWPPSSVNYSYWPKNPQDYREVNEEYTRQVKKLSEKLMEWLSEGLGLRREAIKEAVDGEYLMNINYYPPCPHPDLIEGLDAHTDVSGLTLLLTNEIPGLQVFKDDQWIHVDYIPFAVIINISDQILRVSNGRYKSVLHKTTVDKERTRMSWAALVRPTYDMIVGPFPELISHDNPPKFKPMAYKDYIYRKIRNLPFFDLDS
>Myper.0005s1787.1.v2.1
METKSQEEDSIIQESLLTCIDLANYDLHQSALLLKQACLDCGFFYVMNHGISEEVMDKAFEQSKKFFALPLEEKMKVLKNEKHRGYTPLYDQIPDPENQVHGDHKEGYYIGAEVPRDDPQWDQPFYGPNPWPDPDLLPGWRETMEKYHQEALRVCKATAKLLALALDLDADYFDSPEMLGKPISTLRLLHYEGKSDPSKGIYGTGAHSDYGMMTLLATDGVLGLQICKDRNVKPQKWEYVPSIKGAIVVNLGDMMERWSNCLFKSTLHRVLLNGRNRYSIPFFVEPNHDCIIECLSTCQSENNLPKYPAIKCSKYLTQRYEESHANFSSYEKQA
>Myper.0005s1784.1.v2.1
METKSQEEDSIIQESLLTCIDLANYDLHQSALLLKQACLDCGFFYVMNHGISEEVMDKAFEQSKKFFALPLEEKMKVLKNEKHRGYTPLYDQIPDPENQVHGDHKEGYYIGAEVPRDDPQWDQPFYGPNPWPDPDLLPGWRETMEKYHQEALRVCKATAKLLALALDLDADYFDSPEMLGKPISTLRLLHYEGKSDPSKGIYGTGAHSDYGMMTLLATDGVLGLQICKDRNVKPQKWEYVPSIKGAIVVNLGDMMERWSNCLFKSTLHRVLLNGRNRYSIPFFVEPNHDCIIECLSTCQSENNLPKYPAIKCSKYLTQRYEESHANFSSYEKQA
>Myper.0005s1786.1.v2.1
METKSQEEDSIIQESLLTCIDLANYDLHQSALLLKQACLDCGFFYVMNHGISEEVMDKAFEQSKKFFALPLEEKMKVLKNEKHRGYTPLYDQIPDPENQVHGDHKEGYYIGAEVPRDDPQWDQPFYGPNPWPDPDLLPGWRETMEKYHQEALRVCKATAKLLALALDLDADYFDSPEMLGKPISTLRLLHYEGKSDPSKGIYGTGAHSDYGMMTLLATDGVLGLQICKDRNVKPQKWEYVPSIKGAIVVNLGDMMERWSNCLFKSTLHRVLLNGRNRYSIPFFVEPNHDCIIECLSTCQSENNLPKYPAIKCSKYLTQRYEESHANFSSYEKQA
>Myper.0008s0598.1.v2.1
MAETIPSEFQGTASEGIPVVDLSSDEDLVARAVVKASQEWGIFQVVKHGIPTELIRRLKEVGTEFFELPETEKELVARPADSTDFEGYTTDYKKDGKGRRTWADHLFHRVWPPSRINYRFWPENSPDYRKVNEEYAREIKKLSEKIMGWLSEGLGLSREALKEGLGGETVEYLMKVIFYPPCPDLDLLYGAPHHTDLNGITFLIANQVHGLQAFQDNKWIDVNYDDSGIVVIIADQIKRMSNGRYKCGEHRATMDTDRTRISWPVFAEPSLDHVVGPLPELIIGDDNAPKFKPYVYREYKFRKMNELPLD
>Myper.0008s0599.1.v2.1
MAETIPSGFQGTASEGIPVVDLSNSDEDLVARAVVKASQEWGIFLVVNHGIPTELIRRLKEVGTDFFELPETEKELIARPADSTDLEGYTTGYKKDEEGRKTWVDHFFHRVWPPSRINYRFWLENSPDYREVNEEYAREIKKLSEKIMGWLSEGLGLHREALKEGFGGETVEYLMKVIFYPPCPDLDLLYGTPHHTDLGVMTTLVANQVHGLQALQDNKWIDVNYDDSKIVVIIADQIKRMSNGRYQCGEHRAMMDKDRTRISWPVFVEPNLDHVVGPLPELIIGDDSAPKFKPYAYSEYKFRKLNELPLD
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University