Enzyme
Requires Mg2+ for activity. The enzyme catalyses the methylation of daidzein and genistein. It does not methylate naringenin, apigenin, luteolin or kaempferol.
HI4'OMT
Enzyme
Isoflavone 4'-O-methyltransferase
Enzyme name
EC:2.1.1.46
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:4'-hydroxyisoflavone 4'-O-methyltransferase
Sysname
S-adenosyl-L-methionine + a 4'-hydroxyisoflavone = S-adenosyl-L-homocysteine + a 4'-methoxyisoflavone [RN:R07331]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];4'-methoxyisoflavone [CPD:C03074]
Product
Species information
Morus notabilis
Species name
Morus notabilis is a species of eudicot in the family Moraceae (mulberry family).
Species info
Gene sequence info
Morus_notabilis.HI4_OMT.fa
Download
>rna-XM_010095673.2
MMSSSELLQAFSLLRSCSISHIKSMSLKCAIELGIPYIIHNHGQRAITFSKLIESLPVHPSKTHCIYRIMRLLVHSGFFATQKPDQEEEEEAYTLTNASRLLLTDTPVNMESYFLLPLLPQMIGPWQSLSTWLQSNDHTLFKTVYGGTIWDQLADDPELNYRFNEAMASDSRLISKVILDSEYIRVFEGLKCLVDVGGGSGTLAKAIANTFPHMKCTVFDLPHVVANVQGTTDNLSFIGGNMFSDPIPPADAILLKMIMHNWNDEDGSRILKRCREAILSNGNGGKVIVIDTVIGNEKTQDLTETKLCNDVIMMTLTGKERSLMQWEKLILTAQFSHYKITPIFGSVNSIIEVYP
>rna-XM_010095674.2
MSSSDELIMSSNELVEAHSLLWHSGLDYMKSMSLKCAIELDIANVIHKHGQPIPFSKLVDSLKIPPSKANFLYRIMRILVHHGFFTAQKVNGGKTQEEEEAYSLTNASRLLLTDEPALIMNMKSLSLFFLLPAVVNSWQSWSTWLKNSDDMTLFETAEGKTWWEYASQEPGLNHLFNDAMANDSKLTAKIILEECKEVLEGLKNLVDVGGGTGTMAKAIVNAFPHIKCTVLDLPHVVANVQETSTDNLNFIGGDMFSEQIPPADAILLKNVLHDWTDEACVTILKRCREAVLSNGNKGKIILIEIVVETQKTTNKETTELQLLNDVFFMGLSAKERTLLEWEKLFSAAGISHYKIIHTFGIRSVIEVYP
>rna-XM_024164529.1
MDYFSEDVMSSSELLQAHSLLWSNSVHIVRNLSLKCVIELGIPDIIHKHGQPITLSKLISALPIHPSKAHCIYRIMRILVHSGFFSALKVNGEEEESYSLTNVSRLLLSDGPASIKMKSFFLFHLLPEVVAPLHSLSTWLQQSDGKTFEHGNGKNFWDFLADEPKHIHLFNEAMANDSQLIAKVILTEYKNVFEGLETLVDVGGGTGTMAKAIASSFPHIKCTVFDLPHVVANLEATDNLNFIGGNMFTDTIPPTDGILLKWILHDWNDEDSLTILKRCREAILRNGKGGKVIVIEMVIENQNTNEITGIQLSYDVVMMGSFFGKERNLMEWEKLFFEAGFSHYKINAKMGARSLIELYP
>rna-XM_010111079.2
MAFSEEVMSSSELLQAHSLLWSTSVNYAKYVTVKCAIELGIPDIIHKHGKPITLSKLINALPIHPSKTHCICRIMRILVHSGFFSTGRQVEEEEEEEEESYSLTNASRLLLSEGSDFTKMKSFVLFHLLPEAAAPWHSMSTWLQSSDGSTFEHQKGKTFWDCLADEPSQNRLFNEAMANDSQLIAKLTLTECKDVFEGLTTLVDVGGGTGTMAKAIASTFPHIKCTVFDLPQVVANLEGNDNLNFIGGNMFSDTIPTADAILLKWILHDWNDEESLAILKKCREAILKNEKGGKVIVIETVIDSKNTDEITGIQLCNDMIMMVSFSGKERNLREWEKLFFSAGFSHYKINRKLGVRSLIELYP
>rna-XM_024175289.1
MDLLQGQGSNELFQAQSHLYSHIFRFISSMSLKCAVQLGIPDIISNHGQPITLSELVSALDIHPTKAGFIYRLMRLLVHDGFFTSTKIGENEEEEEEEASYDLTPSSRLLLKDKIPSLSPFLVAMLEPAITTPWHSLGNWFQREEDMTPFESAHGISFWAYGNKNSEFGNLFNQAMASDSGMMNLVVKDCKLLFEGLSSLVDVGGGTGTVSKIITEAFPHLICTVLDLPHVVANLPDTENLKFVGGDMFQDIPSADAIFLKWLVHAWSDEEGLKVLKKCREAIPSNEAIPSNGRGKVIIIDIVINKEKDERELTQGKLYFDLLMMVAVSGRERSKKEWEKLFLEAGFSHFKITPLFGLRSLIEVFH
>rna-XM_010112856.2
MSCSNNCLWCARLWVQLPRDTGRRSLGLKTFLSLSSSIIDLLQGKESNELFQAQSHLYKHMLSFISSMSIKSAVQLSIPDIINNDGQPITLSELASALDIHPTKAGFIHRLMCLLVHHGFFNSAKIGHDEEAYDLTPSSRLLKDKIPSLSPFVMSLLDPALITPWHLLGNWFQKEEKMPPFESAHGMSFWAYVNKNSDFNNHFNQAMAIDSGMMNLVVKDCKPVFEGLSSLVDVGGGTGTISKIIIETFPHIKCTVLDLPHVVANLPATENLKFIGGDMFQGIPSADAILLKLMLHCWSDEECLKVLKICRVAIPSNGGKVIITDIVINKDKDDPELTEGKLYFDLLMMILVTGRERSEKDWEKLFLEAGFSHYKITPLFGLRSLIEVFPRKQLSSNESVHLRTSLISMVARNSMQLFVFLPGLHPKLMNKMQIGSDKTIKNNEYIESADENEVFAITQITVTDCGCGWASN
MMSSSELLQAFSLLRSCSISHIKSMSLKCAIELGIPYIIHNHGQRAITFSKLIESLPVHPSKTHCIYRIMRLLVHSGFFATQKPDQEEEEEAYTLTNASRLLLTDTPVNMESYFLLPLLPQMIGPWQSLSTWLQSNDHTLFKTVYGGTIWDQLADDPELNYRFNEAMASDSRLISKVILDSEYIRVFEGLKCLVDVGGGSGTLAKAIANTFPHMKCTVFDLPHVVANVQGTTDNLSFIGGNMFSDPIPPADAILLKMIMHNWNDEDGSRILKRCREAILSNGNGGKVIVIDTVIGNEKTQDLTETKLCNDVIMMTLTGKERSLMQWEKLILTAQFSHYKITPIFGSVNSIIEVYP
>rna-XM_010095674.2
MSSSDELIMSSNELVEAHSLLWHSGLDYMKSMSLKCAIELDIANVIHKHGQPIPFSKLVDSLKIPPSKANFLYRIMRILVHHGFFTAQKVNGGKTQEEEEAYSLTNASRLLLTDEPALIMNMKSLSLFFLLPAVVNSWQSWSTWLKNSDDMTLFETAEGKTWWEYASQEPGLNHLFNDAMANDSKLTAKIILEECKEVLEGLKNLVDVGGGTGTMAKAIVNAFPHIKCTVLDLPHVVANVQETSTDNLNFIGGDMFSEQIPPADAILLKNVLHDWTDEACVTILKRCREAVLSNGNKGKIILIEIVVETQKTTNKETTELQLLNDVFFMGLSAKERTLLEWEKLFSAAGISHYKIIHTFGIRSVIEVYP
>rna-XM_024164529.1
MDYFSEDVMSSSELLQAHSLLWSNSVHIVRNLSLKCVIELGIPDIIHKHGQPITLSKLISALPIHPSKAHCIYRIMRILVHSGFFSALKVNGEEEESYSLTNVSRLLLSDGPASIKMKSFFLFHLLPEVVAPLHSLSTWLQQSDGKTFEHGNGKNFWDFLADEPKHIHLFNEAMANDSQLIAKVILTEYKNVFEGLETLVDVGGGTGTMAKAIASSFPHIKCTVFDLPHVVANLEATDNLNFIGGNMFTDTIPPTDGILLKWILHDWNDEDSLTILKRCREAILRNGKGGKVIVIEMVIENQNTNEITGIQLSYDVVMMGSFFGKERNLMEWEKLFFEAGFSHYKINAKMGARSLIELYP
>rna-XM_010111079.2
MAFSEEVMSSSELLQAHSLLWSTSVNYAKYVTVKCAIELGIPDIIHKHGKPITLSKLINALPIHPSKTHCICRIMRILVHSGFFSTGRQVEEEEEEEEESYSLTNASRLLLSEGSDFTKMKSFVLFHLLPEAAAPWHSMSTWLQSSDGSTFEHQKGKTFWDCLADEPSQNRLFNEAMANDSQLIAKLTLTECKDVFEGLTTLVDVGGGTGTMAKAIASTFPHIKCTVFDLPQVVANLEGNDNLNFIGGNMFSDTIPTADAILLKWILHDWNDEESLAILKKCREAILKNEKGGKVIVIETVIDSKNTDEITGIQLCNDMIMMVSFSGKERNLREWEKLFFSAGFSHYKINRKLGVRSLIELYP
>rna-XM_024175289.1
MDLLQGQGSNELFQAQSHLYSHIFRFISSMSLKCAVQLGIPDIISNHGQPITLSELVSALDIHPTKAGFIYRLMRLLVHDGFFTSTKIGENEEEEEEEASYDLTPSSRLLLKDKIPSLSPFLVAMLEPAITTPWHSLGNWFQREEDMTPFESAHGISFWAYGNKNSEFGNLFNQAMASDSGMMNLVVKDCKLLFEGLSSLVDVGGGTGTVSKIITEAFPHLICTVLDLPHVVANLPDTENLKFVGGDMFQDIPSADAIFLKWLVHAWSDEEGLKVLKKCREAIPSNEAIPSNGRGKVIIIDIVINKEKDERELTQGKLYFDLLMMVAVSGRERSKKEWEKLFLEAGFSHFKITPLFGLRSLIEVFH
>rna-XM_010112856.2
MSCSNNCLWCARLWVQLPRDTGRRSLGLKTFLSLSSSIIDLLQGKESNELFQAQSHLYKHMLSFISSMSIKSAVQLSIPDIINNDGQPITLSELASALDIHPTKAGFIHRLMCLLVHHGFFNSAKIGHDEEAYDLTPSSRLLKDKIPSLSPFVMSLLDPALITPWHLLGNWFQKEEKMPPFESAHGMSFWAYVNKNSDFNNHFNQAMAIDSGMMNLVVKDCKPVFEGLSSLVDVGGGTGTISKIIIETFPHIKCTVLDLPHVVANLPATENLKFIGGDMFQGIPSADAILLKLMLHCWSDEECLKVLKICRVAIPSNGGKVIITDIVINKDKDDPELTEGKLYFDLLMMILVTGRERSEKDWEKLFLEAGFSHYKITPLFGLRSLIEVFPRKQLSSNESVHLRTSLISMVARNSMQLFVFLPGLHPKLMNKMQIGSDKTIKNNEYIESADENEVFAITQITVTDCGCGWASN
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University