Enzyme
Isolated from the plants Perilla frutescens var. crispa, Verbena hybrida [1], Dahlia variabilis [2] and Gentiana triflora (clustered gentian) [3]. It will also act on anthocyanidin 3-O-(6-O-malonylglucoside) [2] and is much less active with hydroxycinnamoylglucose derivatives [3]. There is no activity in the absence of the 3-O-glucoside group.
PF3R4
Enzyme
Anthocyanidin 3-O-glucoside 5-O-glucosyltransferase 1
Enzyme name
EC:2.4.1.298
Entry
Transferases;Glycosyltransferases;Hexosyltransferases
Class
UDP-alpha-D-glucose:anthocyanidin-3-O-beta-D-glucoside 5-O-glucosyltransferase
Sysname
UDP-alpha-D-glucose + an anthocyanidin 3-O-beta-D-glucoside = UDP + an anthocyanidin 3,5-di-O-beta-D-glucoside [RN:R10262]
Reaction
UDP [CPD:C00015];anthocyanidin 3,5-di-O-beta-D-glucoside [CPD:C06361]
Product
Species information
Mimulus guttatus
Species name
Spotted monkey flower (Erythranthe guttata) is a species of eudicot in the family Phrymaceae.
Species info
Gene sequence info
Mimulus_guttatus.PF3R4.fa
Download
>MgTOL.D1433.1.v5.0
MEQQRHVLLVTFPAQGHINPSLQFAKRLTAIGIKVTFATSLYARRRMSRTAAGLPKGVTFAAFSDGYDDGFNSGNDDAGKYMTEIRSRGSQSLRDTILDAAEQGRPVTQLVYTLLLPWAPEMAREFHVPSALLWIQPATVLNIYYYYFNGYGDEIATNSNDPTWQIQFPGIPALNFSKRDLPSFVLPSSSDKYSFALPTNRSGPLIPSAFLDGNDPSDKSFGGDLFEKSDDNSVELWLNSKPESSVVYVSFGSILKLPKAQMEEIAKGLLESERPFLWVIRINEDNKNEEEEKLSCFDELEKLGKIVPWCSQLEVLTHPSIGCFVSHCGWNSTLESLSSGVPVVAFPQWTDQGTNAKLIEDVWKSGVRVRGVGGDGVVESGEIKRCIEEVMDGGERSLELRANAREWKCLAREAMDENGSSCKNLKAFLEEN
>MgTOL.G0824.1.v5.0
MTEIRSRGSQSLRDTILAAAEQGRPVTQLVYTLLLPWAPEVAREFHVPSALLWIQPATVLNIYYYYFNGYGDEIAANSDDPTWQIQFPGIPALNFSKRDLPSFVLPSSSDKYSFALPSFKEQLETLDAEKTTPKVLVNTFDALEPHALKSIDKYELIGVGPLIPSAPSDKSFRGDLFEKYDDNNVELWLNSKPESSVVYVSFGSILKLPKAQMEEIAKGILESDRPFLWVIRINEDKKNGEEEEEEKLSCLDELEKIGKIVPWCSQLEVLTHPSIGCFVSHCGWNSTLESLSSGVPVVAFPQWTDQGTNAKLIEDVWKSGVRVRVGGDGVVESGEIKRCIEEVMDGAERSRELRANAREWKSLAREAMDENGSSCNNLKAFLEEN
>MgTOL.G0825.1.v5.0
MQNCKTSHHPDNPFVFMFERHVLLVTFPAQGHINPSLQFAKRLTAIGIKVTFATSLYARRRMSKTAAGLPKGVTFAAFSDGYDDGFNPGNDDAGKYMTEIRSRGSQSLRDTIIAAAEQGRPVTQLVYTLLLPWAPEVAPNSNDPTWQIQFPGIPALNFSKRDLPSFILPSSSDKYSFLLPSFKDQLETLDAEKLKPKVLVNTFDALEPHALKSIDNYELIGVGPLIPSAPSDKSFRGDLFEKSDDNSVELWLNSKPESSVVYVSFGSLLKLPKAQMEEIAKGLLESDRPFLWVIRINEDNKNEEEEEKLSCLDELEKIGKFVPWCSQLEVLTHASVGCFVSHCGWNSTLESLSCGVPVVAFPQWTDQGTNAKLIEDAWKSGVRVRGVGGDGVVESGEIKRCLEEVMDGGERSRELRANAREWKSLAREAMDENGSSFNSLKTFLEEN
>MgTOL.L0602.1.v5.0
MKSHHFLIISFPIQGHINPILQLAKNLARLGAKVTFATTDRILHLLQDSLPILNRLSYASFSDGHHHEEQEKTTKSTADYMAELKRAGLLLPWAAAVARDMQIPSAFLSIQCATAFAIYHRFSKDHNEIDSVDILQDLPLFSSSDLPTFLLPDNPMYSFMKPMMIEHMQELEKDHKPLVLLNTFEELERDAIESLKAKNVNVITIGPLIPSAFSDGNDSTDKSFGGDLFVTKNEDYFKWLDSKPEHSVVYVAFGSLVVMNNDQKIEFLHGLVESKRPFLWVIRSSSSDSVDETETKKMIDDNNIGENGMVVEWCAQMEVLSHKSIGCFVTHCGWNSTLEGLICGVPFICCPHFSDQPTNAKLVEEVWGTGVRARANGEVLIEREGFKKCLDVVMGEGERGREMRRNALKWRGLALDAVKENGSTYNNLKRVLK
>MgTOL.N0421.1.v5.0
MAAKNHHVLLVTFPAQGHINPSLQFAKRLAKLGVAVTFLTSSSAVRRMNTTSGGGGGSAINFASFSDGFDGGWSNDETQNFMAALRTHGSKAVEDAVAAAAAENRPFSRVIYTLLVPWAGQVAHRLKVPVTLLWIQPAILFGVYFYYFNKYIDAAANNNDNNNKIIELPGLPPFAPSDLPSFMSASNPSMYDFALPTFTENFEILDRETSPPPPTVLINTFQSLESEALTAITKYKLTAIGPLIPSAFLDGKDPTDTSFGGDLIKKPPSQDYLQYLDSKPKSSVVYVAFGSYSELSKPQMAEIAKGLIKSGKPFLWVIRGAESAEKLDGIFSEIFPGGGSIMYREELERQGKIVGWCTQVEVLSHPSVGCFFTHCGWNSTLESLVCGVPVVGFPQWTDQTTNAKLVEDLWRSGVRVSGGGGGGGGGGGVVGSDEIERCLEVVMGGGDRAAEMRREAEKWRGLAKEAMEEDGSSNVNLKSFVDELLV
>MgTOL.O1054.1.v5.0
MEQQRHVLLVTFPAQGHINPSLQFAKRLTAIGIKVTFATSLYARRRMARTAAGLPKGVTFAAFSDGYDDGFKPGNDDAGKYMTEIRSRGSQSLRDTILAAAEQGRPVTQLVYTLLLPWAPEVAREFHVPSALLWIQPATVLNIYYYYFNGYGDEITANSNDPTWQIQFPGITALNFSKRDLPSFVLPSSSDKYSFALPSFKEQLETLDAEILKPKVLVNTFDALEPHALKSIDKYELFGVGPLIPSAPSDKSFRGDLFEKSDDNSVELWLNSKPESSVVYVSFGSILKLPKAQMEKVAKGLLESDRPFLWVIRINEDKKNEEEEEEKLSCLDELEKIGKIVPWCSQLEVLTHPSIGCFVSHCGWNSTLESLSSGVPVVAFPQWTDQGTNAKLIEDVWKSGVRVRGVGGDGVVESGEIKRCIEEVMDGGKRSRELRANAREWKSLARGAMDENGSSCMNLKDFLDEN
>MgTOL.O1053.1.v5.0
MEQQRHVLLVTFPVQGHINPSLQFAKRLTAIGIKVTFATSLYARRRMSRTAAGLPKGVTFAAFSDGYDDGFNPGNDDAGKYMTEIRSRGSQSLRDTILAAAEQGCPVTQLVYTLLLPWAPEVAREFHVPSALLWIQPATVLNIYYYYFNGYGDEIAANSNDATWQIQFPGIPALNFSKRDLPSFILPSSSDKYSVELPSFKEQLETLDAEKTKPKVLVNTFDALEPHALKSIDNYELIGVGPLIPSAPSDKSFRGDLFEKSDDNSVELWLNSKPESSVVYVSFGSILKLPKAQMEEIAKGLLESDRPFLWVIRINEDKKNEEEEEEKLSCFDELEKIGKIVPWCSQLEVLTHPSIGCFVSHCGWNSTLESLSSGVPVVAFPQWTDQGTNAKLIEDVWMSGVRVRVGGDGVVESGEIKRCIEEVMDGAERSRELRANAREWKSLAREAMDENGSSFNNLKAFLEEN
>MgTOL.O1098.1.v5.0
MEQQRHVLLVTFPAQGHINPSLQFAKRLTAIGIKVTFATSLYARRRMSRTAAGLPKGVTFAAFSDGYDDGSRGSQSLRDSILAAAEQGRPVTQLVYTLLLPWAPEVAREFHVPSALLWIQPATVLNIYYYYFNGYGDEIATNSNDPTWQIQFPGIPALNFSKRDLPSFVLPSSSDKYSFALPSFKEQLETLDAEKMKPKVLVNTFDALEPHALKSIDNYELIGVGPLIPSAFLDGNDPSDKSFGGDLFEKSDDNSVELWLNSKLESSVVYVSFGSILKLPKAQMEEIAKGILESERPFLWVIRINEDNKNEEEEKLSCFDELEKLGKIVPWCSQLEVLTHPSIGCFVSHCGWNSTLESLSSGVPVVAFPQWTDQGTNAKLIEDVWKSGVRVRGVGGDGVVESGEIKRCIEEVMDGGERSLELRANAREWKCLAREAMDENGSSCKNLKAFLEEN
>MgTOL.O0142.1.v5.0
MEQQRHVLLVTFPAQGHINPSLQFAKRLTAIGIKVTFATSLYARRRMSRTAAGLPKGVTFAAFSDGYDDGFNPGNDDAGKYMTEIRSRGSQSLRDTILAAAEQGRPVTQLVYTLLLPWAPEVAREFHVPSALLWIQPATVLNIYYYYFNGYGDEIVANSNDPTWQIQFPGIPALNFSKRDLPSFILPSSSDKYSFALPSFKEQLETLDAEKTKPKVLVNTFDALEPHALKSIDNYELIGVGPLIPSAFLDGKDPSDKSFGGDLFEKSDDNNVELWLNSKPESSVVYVSFGSILKLPKAQMEEIAKGLLQSNRPFLWVIRINEDNKKNEEEDDEEKLSCLDELEKIGKIVPWCSQLEVLTHASVGCFVSHCGWNSTLESLSCGVPWWLSSMDRSRTNAKLIEDAWKRGLEWEELVEMGGGEW
MEQQRHVLLVTFPAQGHINPSLQFAKRLTAIGIKVTFATSLYARRRMSRTAAGLPKGVTFAAFSDGYDDGFNSGNDDAGKYMTEIRSRGSQSLRDTILDAAEQGRPVTQLVYTLLLPWAPEMAREFHVPSALLWIQPATVLNIYYYYFNGYGDEIATNSNDPTWQIQFPGIPALNFSKRDLPSFVLPSSSDKYSFALPTNRSGPLIPSAFLDGNDPSDKSFGGDLFEKSDDNSVELWLNSKPESSVVYVSFGSILKLPKAQMEEIAKGLLESERPFLWVIRINEDNKNEEEEKLSCFDELEKLGKIVPWCSQLEVLTHPSIGCFVSHCGWNSTLESLSSGVPVVAFPQWTDQGTNAKLIEDVWKSGVRVRGVGGDGVVESGEIKRCIEEVMDGGERSLELRANAREWKCLAREAMDENGSSCKNLKAFLEEN
>MgTOL.G0824.1.v5.0
MTEIRSRGSQSLRDTILAAAEQGRPVTQLVYTLLLPWAPEVAREFHVPSALLWIQPATVLNIYYYYFNGYGDEIAANSDDPTWQIQFPGIPALNFSKRDLPSFVLPSSSDKYSFALPSFKEQLETLDAEKTTPKVLVNTFDALEPHALKSIDKYELIGVGPLIPSAPSDKSFRGDLFEKYDDNNVELWLNSKPESSVVYVSFGSILKLPKAQMEEIAKGILESDRPFLWVIRINEDKKNGEEEEEEKLSCLDELEKIGKIVPWCSQLEVLTHPSIGCFVSHCGWNSTLESLSSGVPVVAFPQWTDQGTNAKLIEDVWKSGVRVRVGGDGVVESGEIKRCIEEVMDGAERSRELRANAREWKSLAREAMDENGSSCNNLKAFLEEN
>MgTOL.G0825.1.v5.0
MQNCKTSHHPDNPFVFMFERHVLLVTFPAQGHINPSLQFAKRLTAIGIKVTFATSLYARRRMSKTAAGLPKGVTFAAFSDGYDDGFNPGNDDAGKYMTEIRSRGSQSLRDTIIAAAEQGRPVTQLVYTLLLPWAPEVAPNSNDPTWQIQFPGIPALNFSKRDLPSFILPSSSDKYSFLLPSFKDQLETLDAEKLKPKVLVNTFDALEPHALKSIDNYELIGVGPLIPSAPSDKSFRGDLFEKSDDNSVELWLNSKPESSVVYVSFGSLLKLPKAQMEEIAKGLLESDRPFLWVIRINEDNKNEEEEEKLSCLDELEKIGKFVPWCSQLEVLTHASVGCFVSHCGWNSTLESLSCGVPVVAFPQWTDQGTNAKLIEDAWKSGVRVRGVGGDGVVESGEIKRCLEEVMDGGERSRELRANAREWKSLAREAMDENGSSFNSLKTFLEEN
>MgTOL.L0602.1.v5.0
MKSHHFLIISFPIQGHINPILQLAKNLARLGAKVTFATTDRILHLLQDSLPILNRLSYASFSDGHHHEEQEKTTKSTADYMAELKRAGLLLPWAAAVARDMQIPSAFLSIQCATAFAIYHRFSKDHNEIDSVDILQDLPLFSSSDLPTFLLPDNPMYSFMKPMMIEHMQELEKDHKPLVLLNTFEELERDAIESLKAKNVNVITIGPLIPSAFSDGNDSTDKSFGGDLFVTKNEDYFKWLDSKPEHSVVYVAFGSLVVMNNDQKIEFLHGLVESKRPFLWVIRSSSSDSVDETETKKMIDDNNIGENGMVVEWCAQMEVLSHKSIGCFVTHCGWNSTLEGLICGVPFICCPHFSDQPTNAKLVEEVWGTGVRARANGEVLIEREGFKKCLDVVMGEGERGREMRRNALKWRGLALDAVKENGSTYNNLKRVLK
>MgTOL.N0421.1.v5.0
MAAKNHHVLLVTFPAQGHINPSLQFAKRLAKLGVAVTFLTSSSAVRRMNTTSGGGGGSAINFASFSDGFDGGWSNDETQNFMAALRTHGSKAVEDAVAAAAAENRPFSRVIYTLLVPWAGQVAHRLKVPVTLLWIQPAILFGVYFYYFNKYIDAAANNNDNNNKIIELPGLPPFAPSDLPSFMSASNPSMYDFALPTFTENFEILDRETSPPPPTVLINTFQSLESEALTAITKYKLTAIGPLIPSAFLDGKDPTDTSFGGDLIKKPPSQDYLQYLDSKPKSSVVYVAFGSYSELSKPQMAEIAKGLIKSGKPFLWVIRGAESAEKLDGIFSEIFPGGGSIMYREELERQGKIVGWCTQVEVLSHPSVGCFFTHCGWNSTLESLVCGVPVVGFPQWTDQTTNAKLVEDLWRSGVRVSGGGGGGGGGGGVVGSDEIERCLEVVMGGGDRAAEMRREAEKWRGLAKEAMEEDGSSNVNLKSFVDELLV
>MgTOL.O1054.1.v5.0
MEQQRHVLLVTFPAQGHINPSLQFAKRLTAIGIKVTFATSLYARRRMARTAAGLPKGVTFAAFSDGYDDGFKPGNDDAGKYMTEIRSRGSQSLRDTILAAAEQGRPVTQLVYTLLLPWAPEVAREFHVPSALLWIQPATVLNIYYYYFNGYGDEITANSNDPTWQIQFPGITALNFSKRDLPSFVLPSSSDKYSFALPSFKEQLETLDAEILKPKVLVNTFDALEPHALKSIDKYELFGVGPLIPSAPSDKSFRGDLFEKSDDNSVELWLNSKPESSVVYVSFGSILKLPKAQMEKVAKGLLESDRPFLWVIRINEDKKNEEEEEEKLSCLDELEKIGKIVPWCSQLEVLTHPSIGCFVSHCGWNSTLESLSSGVPVVAFPQWTDQGTNAKLIEDVWKSGVRVRGVGGDGVVESGEIKRCIEEVMDGGKRSRELRANAREWKSLARGAMDENGSSCMNLKDFLDEN
>MgTOL.O1053.1.v5.0
MEQQRHVLLVTFPVQGHINPSLQFAKRLTAIGIKVTFATSLYARRRMSRTAAGLPKGVTFAAFSDGYDDGFNPGNDDAGKYMTEIRSRGSQSLRDTILAAAEQGCPVTQLVYTLLLPWAPEVAREFHVPSALLWIQPATVLNIYYYYFNGYGDEIAANSNDATWQIQFPGIPALNFSKRDLPSFILPSSSDKYSVELPSFKEQLETLDAEKTKPKVLVNTFDALEPHALKSIDNYELIGVGPLIPSAPSDKSFRGDLFEKSDDNSVELWLNSKPESSVVYVSFGSILKLPKAQMEEIAKGLLESDRPFLWVIRINEDKKNEEEEEEKLSCFDELEKIGKIVPWCSQLEVLTHPSIGCFVSHCGWNSTLESLSSGVPVVAFPQWTDQGTNAKLIEDVWMSGVRVRVGGDGVVESGEIKRCIEEVMDGAERSRELRANAREWKSLAREAMDENGSSFNNLKAFLEEN
>MgTOL.O1098.1.v5.0
MEQQRHVLLVTFPAQGHINPSLQFAKRLTAIGIKVTFATSLYARRRMSRTAAGLPKGVTFAAFSDGYDDGSRGSQSLRDSILAAAEQGRPVTQLVYTLLLPWAPEVAREFHVPSALLWIQPATVLNIYYYYFNGYGDEIATNSNDPTWQIQFPGIPALNFSKRDLPSFVLPSSSDKYSFALPSFKEQLETLDAEKMKPKVLVNTFDALEPHALKSIDNYELIGVGPLIPSAFLDGNDPSDKSFGGDLFEKSDDNSVELWLNSKLESSVVYVSFGSILKLPKAQMEEIAKGILESERPFLWVIRINEDNKNEEEEKLSCFDELEKLGKIVPWCSQLEVLTHPSIGCFVSHCGWNSTLESLSSGVPVVAFPQWTDQGTNAKLIEDVWKSGVRVRGVGGDGVVESGEIKRCIEEVMDGGERSLELRANAREWKCLAREAMDENGSSCKNLKAFLEEN
>MgTOL.O0142.1.v5.0
MEQQRHVLLVTFPAQGHINPSLQFAKRLTAIGIKVTFATSLYARRRMSRTAAGLPKGVTFAAFSDGYDDGFNPGNDDAGKYMTEIRSRGSQSLRDTILAAAEQGRPVTQLVYTLLLPWAPEVAREFHVPSALLWIQPATVLNIYYYYFNGYGDEIVANSNDPTWQIQFPGIPALNFSKRDLPSFILPSSSDKYSFALPSFKEQLETLDAEKTKPKVLVNTFDALEPHALKSIDNYELIGVGPLIPSAFLDGKDPSDKSFGGDLFEKSDDNNVELWLNSKPESSVVYVSFGSILKLPKAQMEEIAKGLLQSNRPFLWVIRINEDNKKNEEEDDEEKLSCLDELEKIGKIVPWCSQLEVLTHASVGCFVSHCGWNSTLESLSCGVPWWLSSMDRSRTNAKLIEDAWKRGLEWEELVEMGGGEW
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University