Enzyme
Specific for quercetin. Related enzymes bring about the 3-O-methylation of other flavonols, such as galangin and kaempferol.
COMT
Enzyme
Flavone 3'-O-methyltransferase
Enzyme name
EC:2.1.1.76
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:3,5,7,3',4'-pentahydroxyflavone 3-O-methyltransferase
Sysname
S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone = S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone [RN:R02157]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];3-methoxy-5,7,3',4'-tetrahydroxyflavone [CPD:C04443]
Product
Species information
Mimulus guttatus
Species name
Spotted monkey flower (Erythranthe guttata) is a species of eudicot in the family Phrymaceae.
Species info
Gene sequence info
Mimulus_guttatus.COMT.fa
Download
>MgTOL.C1084.1.v5.0
MEGAFTKHAEEDEAAVVAMAACNSYMLPMTLSAAVELDLFEIIAGAGDGAHLSASDVASRLTTAADGAAVVLGSMMQLLAANSLLTCSVDVLADGGVERRYGLSPAGKFFAKDEGGASLCQSVSYTTRVAGGALGFSVKDLVLFLVREGGNMFERMNVKSFYELIMRDVETGKVFNDSLGARSAIVMKKVVKTYQGFEGLSRLVDVGGGSGSTLDAIVSEYPSIHGVNFDLPHVVNTSPSYNNIEHIGGDMFLEVPKGDAILLKNILHNWGDEQCVNLLKCCYKSLPNKGKLIVMDYIVPNTPQTDVHAKYASQMNLTMKILVGGKERTEDEFQGMAIAAGFAEFKVVCYVYGIWIMEFIKLV
>MgTOL.E0697.1.v5.0
MNPPTQNDDSQSSYAIHLVNASVLPMALKACIELDVLEIIHRAGPHGPLSSFQIASQIPNCHNPHAASNLLDRMLALLASYSILTCSVSNGPQKLYSLGPVSKYFVANANGVSLAPLFLLNQDKVMMDTWHHMKDAVIEGGLPPFNRAYGMDASEYIVKKNPSYFQLFKNSMSNYNSMFMEKLMDTYRGFEGLESIVDVGGGDGSVLNTIVGKYPNIKGTNFELASVIDNNKSVYPGIEYISGDMFISIPKGDAIFLKWMLHSWDDDNCVRILKNCYNALPENGKVIIVELVVPEIPETRAEVQSKLQFDLIMMNMNPNGKERTITEFDVLAQASGFSTTKLQQPSIFDFSLLEFHK
>MgTOL.F1373.1.v5.0
MDYRSEEDAFVFATQLAMAAVFPAALKTAVELDLFELIKNSGAAVSARELAAQLPAAANPDAHVMLNRILRLLTSYSVLNSNPRNLPGGSGGVDL
>MgTOL.F0167.1.v5.0
MGSTGKIVEVSTMVDSSSEEEEACLFALQLASASVLPMVLKSAIELDLLELIKKAGPGAFVSPAELAAQIPTTNPDAHVMLDRILRLLASYSILNCSVKTLPGGGGGVERLYALAPVCKYLTKNDDGVSMAPLLLMNHDKILMESWYHLKDTVLEGGIPFNKAYGMSAFEYHGKDTRFNKVFNQGMSNHSTIIMKKILGTYDGFEGLDTVVDVGGGTGATLNMIISKYPSIRGINFDLPHVIEDAPSYPGVEHAGGDMFVSVPKGDAIFMKWICHDGVMSTV
>MgTOL.G0700.1.v5.0
MEVVNNKYKYGEDDDAAVTALAACSSYILETVLYAAVELDIFEILSRAGDGARLSTSDIVSRLPTPAADGAAVVLDSMLKLLATHSLLICDNIGGPERRYGIAPAGKFFANDDGGASFCQYLLFRPEGASTVPSLKDVIVEGAMRSHSAVLMKKVVKTYRGFEGLSCLVDVGGGTGTALATIISKYPFIRGINFDLPHIVNTAPYYNGIEHIGGDMFVQVPKGDAILLKFILHNWSDEECGKLLKCCYKSLPNKGKLIVMDSIMPNTTQTDIHAMYASQMDFVMTAMFGGKERTHDEFQDLAVKSGFGEFKVVSYAYGMWIMEFIK
>MgTOL.G1327.1.v5.0
MEVVNSNKYKHGEDDEAGVLALAACLSHTFPMALAAAIDLDLFEIISGAGDGARMSTSDIAAQLPSAADGAAAVLDAMLQLLASHSLLICSTNILTDGGGGAVERRYGIAPAGKFFVKDESGVSFAQFHSIVRKGGEIGFSLKDAVIEGGNQFEKICAKSFYEFITSNVEATKMFNDGMRAHSSVIIKKVVKTYRGFEGLSCLVDVGGGSGSSLDTIISKYPSIRGVNFDLPHVVETAPTYNGIEHIGGDMFVEIPKGDAILLKFILHNWGDEQCGNLLNNCYKSLPNNGKLIVMDYILPNTHQDDIHAKYASQMNLTMPCFLEGKERTKEEFEGMAKKAGFVEFKVVCYVYGMWVMEFIKFV
>MgTOL.G0753.1.v5.0
MDCRSEEDAFVFATQLAMAAVFPAALKTAVELDLFELIKKSGGAAVSARELAAQLPASANPDAHVMLDRILRLLTSYSVLNSSPRNLAGGGGGDELVYYLAPMGKYLTKNEDGVCVSPFLMLIQDQVLQKSWDNLKDAVLDGGVPFVKAYGMNVFEYGDTDPRFNKVFDQAMSNYSTIVVKKILEIYDGFEGIQSVVDVGGGTGAILNMIISKYPSIKGINFDLPHVIQHAPSYPGVEHIGGDMFVSVPKGDAIFMKWIVHDWSDEQCLILLKNCHSSLQENGKVILAELIMPEEPDKSVATKTVLHSDVLMLTHNPGGRERTEKEFSHLG
>MgTOL.G0751.1.v5.0
MLDRILRLLTSYSVLNSSPKNLPGSDGVDLVYTLAPVCKYLTKNEDGVCVSPFLLLIQDQVLQKSWDNLKDVVLDGGVPFAKAYGMNVFEYGDKDPRFNKVFDQAMSNYSTIVVKKILEIYDGFEGIESVEDVGGGTGAILNMIISMYPSIKGINFDLPHVIQHAPSYPGVEHIGGDMFVSVPKGDAIFMKWIVHDWSDEQCLILLKNCHNSLQENGKVILAELIMPEEPDKSVATKTVLHSDVLMLTHNPGGRERTEKEFHTLDKAAGFREFRKVCCPHNIWIMELVK
>MgTOL.G1329.1.v5.0
MEVVNSNEYKHGEDDEAGVLALAACLSHTFPMALAAAIDLDLFEIISGAGDGARMLASDIAAQLPSAADWAATVLDAMLQLLASHSLLICSTNILADGGGAVQRRYGIAPAGKFFVKDESGVSFAQFHSIVRKGGEIGFSLKDAVIEGGNQFEKICAKSFYEFITSNVEATKMFNDGMRAHSSVIIKKVVKTYPGFEGLSCLVDVGGGSGSSLETIISKYPSIRGVNFDLPHVVETGPTYNGIEHIGGDMFVEIPKGDAILLKFILHNWGDGQCIILLNNCYKSLPNKGKLIVMDYIMPNTHQDDIHAKYASQMNLMMSCFLEGKERTKEEFEGMAMKAGFVEFKVVCYVYGMWVMEFIKCV
>MgTOL.O0725.1.v5.0
MEVVNNNYKHGGEDDDAAVLALAACGSSILQMVLTAAVELDLFEIIAGAGGGARLSASDIVSRLPTAVDRAAVVLESMMKVLAAHSLLTCENGGGSERRYGISPAGKFFVNDEGGASFRQYLLFSPQGASTGPSLKDIVLGGGNVSEKLFGKSFYEQMESNVDLSKKFNEAMGSHSAVLMKKVVKTYRGFEGLSCLVDVGGGTGTALATIISNNPSIRGINFDLPHIVKTASPYNGIEHIGGDMFVQVPKGDAILLKFILHNWGDEECGNLLKCCYKSLPKEKGKMIVMDYIMPNTPQTDIHAMYASQMDFTMTALLGGKERTEDEFQALAINAGFREFKVVSYVYGMWIMEFIK
>MgTOL.O0036.1.v5.0
MDCRSEEDAFVFATQLAMAAVFPAALKIAVELDLFELIKKSGATVSARELAAQLPASANPDAHVMLDRILRLLTSYSVLNSSPRNLAGGGGGDELVYYLAPVGKYLTKNEDGVCVSPFLMLIQDQVLQKSWDNLKDAVLDGYGDTDPRFNKVFDQAMSNYSTIVVKKILEIYDGFEGIQSVVDVGGGTGAILNMIISKYPSIKASISTYRMLFNMPRRVEHIGGDMFVSVPKGDAIFMKWIVHDWSDEQCLILLKNCHSSLQENGKVILAELIMPEEPDKSVATKTVLHSDVLMLTHNPGGRERTEKEFHTLAKAAGFREFRKVCSPHNIWIMELV
>MgTOL.O0717.1.v5.0
MKMEFVYLPFLMLIQDQVLQKSWDNLKDAVLDGGVPFVKAYGMNVFEYGDTDPRFNKVFDQAMSNYSTIVVKKILEIYDGFEGIQSVVDVGGGTGAILNMIISKYPSIKGINFDLPHVIQHAPSYPGVEHIGETCLFSVPKGDAIS
MEGAFTKHAEEDEAAVVAMAACNSYMLPMTLSAAVELDLFEIIAGAGDGAHLSASDVASRLTTAADGAAVVLGSMMQLLAANSLLTCSVDVLADGGVERRYGLSPAGKFFAKDEGGASLCQSVSYTTRVAGGALGFSVKDLVLFLVREGGNMFERMNVKSFYELIMRDVETGKVFNDSLGARSAIVMKKVVKTYQGFEGLSRLVDVGGGSGSTLDAIVSEYPSIHGVNFDLPHVVNTSPSYNNIEHIGGDMFLEVPKGDAILLKNILHNWGDEQCVNLLKCCYKSLPNKGKLIVMDYIVPNTPQTDVHAKYASQMNLTMKILVGGKERTEDEFQGMAIAAGFAEFKVVCYVYGIWIMEFIKLV
>MgTOL.E0697.1.v5.0
MNPPTQNDDSQSSYAIHLVNASVLPMALKACIELDVLEIIHRAGPHGPLSSFQIASQIPNCHNPHAASNLLDRMLALLASYSILTCSVSNGPQKLYSLGPVSKYFVANANGVSLAPLFLLNQDKVMMDTWHHMKDAVIEGGLPPFNRAYGMDASEYIVKKNPSYFQLFKNSMSNYNSMFMEKLMDTYRGFEGLESIVDVGGGDGSVLNTIVGKYPNIKGTNFELASVIDNNKSVYPGIEYISGDMFISIPKGDAIFLKWMLHSWDDDNCVRILKNCYNALPENGKVIIVELVVPEIPETRAEVQSKLQFDLIMMNMNPNGKERTITEFDVLAQASGFSTTKLQQPSIFDFSLLEFHK
>MgTOL.F1373.1.v5.0
MDYRSEEDAFVFATQLAMAAVFPAALKTAVELDLFELIKNSGAAVSARELAAQLPAAANPDAHVMLNRILRLLTSYSVLNSNPRNLPGGSGGVDL
>MgTOL.F0167.1.v5.0
MGSTGKIVEVSTMVDSSSEEEEACLFALQLASASVLPMVLKSAIELDLLELIKKAGPGAFVSPAELAAQIPTTNPDAHVMLDRILRLLASYSILNCSVKTLPGGGGGVERLYALAPVCKYLTKNDDGVSMAPLLLMNHDKILMESWYHLKDTVLEGGIPFNKAYGMSAFEYHGKDTRFNKVFNQGMSNHSTIIMKKILGTYDGFEGLDTVVDVGGGTGATLNMIISKYPSIRGINFDLPHVIEDAPSYPGVEHAGGDMFVSVPKGDAIFMKWICHDGVMSTV
>MgTOL.G0700.1.v5.0
MEVVNNKYKYGEDDDAAVTALAACSSYILETVLYAAVELDIFEILSRAGDGARLSTSDIVSRLPTPAADGAAVVLDSMLKLLATHSLLICDNIGGPERRYGIAPAGKFFANDDGGASFCQYLLFRPEGASTVPSLKDVIVEGAMRSHSAVLMKKVVKTYRGFEGLSCLVDVGGGTGTALATIISKYPFIRGINFDLPHIVNTAPYYNGIEHIGGDMFVQVPKGDAILLKFILHNWSDEECGKLLKCCYKSLPNKGKLIVMDSIMPNTTQTDIHAMYASQMDFVMTAMFGGKERTHDEFQDLAVKSGFGEFKVVSYAYGMWIMEFIK
>MgTOL.G1327.1.v5.0
MEVVNSNKYKHGEDDEAGVLALAACLSHTFPMALAAAIDLDLFEIISGAGDGARMSTSDIAAQLPSAADGAAAVLDAMLQLLASHSLLICSTNILTDGGGGAVERRYGIAPAGKFFVKDESGVSFAQFHSIVRKGGEIGFSLKDAVIEGGNQFEKICAKSFYEFITSNVEATKMFNDGMRAHSSVIIKKVVKTYRGFEGLSCLVDVGGGSGSSLDTIISKYPSIRGVNFDLPHVVETAPTYNGIEHIGGDMFVEIPKGDAILLKFILHNWGDEQCGNLLNNCYKSLPNNGKLIVMDYILPNTHQDDIHAKYASQMNLTMPCFLEGKERTKEEFEGMAKKAGFVEFKVVCYVYGMWVMEFIKFV
>MgTOL.G0753.1.v5.0
MDCRSEEDAFVFATQLAMAAVFPAALKTAVELDLFELIKKSGGAAVSARELAAQLPASANPDAHVMLDRILRLLTSYSVLNSSPRNLAGGGGGDELVYYLAPMGKYLTKNEDGVCVSPFLMLIQDQVLQKSWDNLKDAVLDGGVPFVKAYGMNVFEYGDTDPRFNKVFDQAMSNYSTIVVKKILEIYDGFEGIQSVVDVGGGTGAILNMIISKYPSIKGINFDLPHVIQHAPSYPGVEHIGGDMFVSVPKGDAIFMKWIVHDWSDEQCLILLKNCHSSLQENGKVILAELIMPEEPDKSVATKTVLHSDVLMLTHNPGGRERTEKEFSHLG
>MgTOL.G0751.1.v5.0
MLDRILRLLTSYSVLNSSPKNLPGSDGVDLVYTLAPVCKYLTKNEDGVCVSPFLLLIQDQVLQKSWDNLKDVVLDGGVPFAKAYGMNVFEYGDKDPRFNKVFDQAMSNYSTIVVKKILEIYDGFEGIESVEDVGGGTGAILNMIISMYPSIKGINFDLPHVIQHAPSYPGVEHIGGDMFVSVPKGDAIFMKWIVHDWSDEQCLILLKNCHNSLQENGKVILAELIMPEEPDKSVATKTVLHSDVLMLTHNPGGRERTEKEFHTLDKAAGFREFRKVCCPHNIWIMELVK
>MgTOL.G1329.1.v5.0
MEVVNSNEYKHGEDDEAGVLALAACLSHTFPMALAAAIDLDLFEIISGAGDGARMLASDIAAQLPSAADWAATVLDAMLQLLASHSLLICSTNILADGGGAVQRRYGIAPAGKFFVKDESGVSFAQFHSIVRKGGEIGFSLKDAVIEGGNQFEKICAKSFYEFITSNVEATKMFNDGMRAHSSVIIKKVVKTYPGFEGLSCLVDVGGGSGSSLETIISKYPSIRGVNFDLPHVVETGPTYNGIEHIGGDMFVEIPKGDAILLKFILHNWGDGQCIILLNNCYKSLPNKGKLIVMDYIMPNTHQDDIHAKYASQMNLMMSCFLEGKERTKEEFEGMAMKAGFVEFKVVCYVYGMWVMEFIKCV
>MgTOL.O0725.1.v5.0
MEVVNNNYKHGGEDDDAAVLALAACGSSILQMVLTAAVELDLFEIIAGAGGGARLSASDIVSRLPTAVDRAAVVLESMMKVLAAHSLLTCENGGGSERRYGISPAGKFFVNDEGGASFRQYLLFSPQGASTGPSLKDIVLGGGNVSEKLFGKSFYEQMESNVDLSKKFNEAMGSHSAVLMKKVVKTYRGFEGLSCLVDVGGGTGTALATIISNNPSIRGINFDLPHIVKTASPYNGIEHIGGDMFVQVPKGDAILLKFILHNWGDEECGNLLKCCYKSLPKEKGKMIVMDYIMPNTPQTDIHAMYASQMDFTMTALLGGKERTEDEFQALAINAGFREFKVVSYVYGMWIMEFIK
>MgTOL.O0036.1.v5.0
MDCRSEEDAFVFATQLAMAAVFPAALKIAVELDLFELIKKSGATVSARELAAQLPASANPDAHVMLDRILRLLTSYSVLNSSPRNLAGGGGGDELVYYLAPVGKYLTKNEDGVCVSPFLMLIQDQVLQKSWDNLKDAVLDGYGDTDPRFNKVFDQAMSNYSTIVVKKILEIYDGFEGIQSVVDVGGGTGAILNMIISKYPSIKASISTYRMLFNMPRRVEHIGGDMFVSVPKGDAIFMKWIVHDWSDEQCLILLKNCHSSLQENGKVILAELIMPEEPDKSVATKTVLHSDVLMLTHNPGGRERTEKEFHTLAKAAGFREFRKVCSPHNIWIMELV
>MgTOL.O0717.1.v5.0
MKMEFVYLPFLMLIQDQVLQKSWDNLKDAVLDGGVPFVKAYGMNVFEYGDTDPRFNKVFDQAMSNYSTIVVKKILEIYDGFEGIQSVVDVGGGTGAILNMIISKYPSIKGINFDLPHVIQHAPSYPGVEHIGETCLFSVPKGDAIS
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University