Enzyme
In addition to the desaturation of (2R,3R)-dihydroflavonols to flavonols, the enzyme from Citrus unshiu (satsuma mandarin) also has a non-specific activity that trans-hydroxylates the flavanones (2S)-naringenin and the unnatural (2R)-naringenin at C-3 to kaempferol and (2R,3R)-dihydrokaempferol, respectively [2]. Requires Fe2+.
FLS
Enzyme
Flavonol synthase
Enzyme name
EC:1.14.20.6
Entry
Oxidoreductases;Acting on paired donors, with incorporation or reduction of molecular oxygen;With 2-oxoglutarate as one donor, and the other dehydrogenated
Class
dihydroflavonol,2-oxoglutarate:oxygen oxidoreductase
Sysname
a dihydroflavonol + 2-oxoglutarate + O2 = a flavonol + succinate + CO2 + H2O [RN:R07368]
Reaction
flavonol [CPD:C01495];succinate [CPD:C00042];CO2 [CPD:C00011];H2O [CPD:C00001]
Product
Species information
Mikania micrantha
Species name
Bittervine (Mikania micrantha) is a species of eudicot in the family Asteraceae (daisy family).
Species info
Gene sequence info
Mikania_micrantha.FLS.fa
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>rna-gnl|WGS:SZYD|E3N88_mrna18333
MDENGELKTTKVPVVQEVARRGIFPQRFIASVAELAGAMETPPVIDIAKLQSDCSRTEELQRLAQAAKDWGLFLIKNHGVVLEDAKDVVKSFFALTFEEKKLTVGSYNNVDNMGYGKNFVKSQHQPLDWIDRLTMIAAPPVTNGLSVWPHKPPNFRFI
>rna-gnl|WGS:SZYD|E3N88_mrna42331
MEVERVQEIASLSNLKGTIPSEFIRLETEQPASTTIQGVVLEVPVIDLSNPDHESLVASICEASKIWGIFQVINHGIPSELISELQKVGKEFFELPQEEKELIAKPIGYQGIEGYGTKLQKEVEGKKGWVDHLFHRVWPPSVINYHFWPKNPPSYRFHLFHHAFSISCMFNLENMFPIC
>rna-gnl|WGS:SZYD|E3N88_mrna42333
MVERLQGDEGDEMTILPLIETNEQYAQNLIPVADKLLGWLSRGLGLEDGEMKQGLGGEDLTYMLKINYYPPCPCPDLALGVAPHTDMSAITILVPNEVQGLQVFKDDHWYDVAYIPNALIIHIGDQVQILSNGKYKSVYHRTTVNKDKTRMSWPMFLEPPLEFEVGPIPKLISQDNPPKYKTKKFKDYVYCKLNKLSQ
MDENGELKTTKVPVVQEVARRGIFPQRFIASVAELAGAMETPPVIDIAKLQSDCSRTEELQRLAQAAKDWGLFLIKNHGVVLEDAKDVVKSFFALTFEEKKLTVGSYNNVDNMGYGKNFVKSQHQPLDWIDRLTMIAAPPVTNGLSVWPHKPPNFRFI
>rna-gnl|WGS:SZYD|E3N88_mrna42331
MEVERVQEIASLSNLKGTIPSEFIRLETEQPASTTIQGVVLEVPVIDLSNPDHESLVASICEASKIWGIFQVINHGIPSELISELQKVGKEFFELPQEEKELIAKPIGYQGIEGYGTKLQKEVEGKKGWVDHLFHRVWPPSVINYHFWPKNPPSYRFHLFHHAFSISCMFNLENMFPIC
>rna-gnl|WGS:SZYD|E3N88_mrna42333
MVERLQGDEGDEMTILPLIETNEQYAQNLIPVADKLLGWLSRGLGLEDGEMKQGLGGEDLTYMLKINYYPPCPCPDLALGVAPHTDMSAITILVPNEVQGLQVFKDDHWYDVAYIPNALIIHIGDQVQILSNGKYKSVYHRTTVNKDKTRMSWPMFLEPPLEFEVGPIPKLISQDNPPKYKTKKFKDYVYCKLNKLSQ
Sheng jun & Dong yang Teams, Yunnan agriculture University