Enzyme
The enzyme catalyses the biosynthesis of pterostilbene from resveratrol.
ROMT
Enzyme
Trans-resveratrol di-O-methyltransferase
Enzyme name
EC:2.1.1.240
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:trans-resveratrol 3,5-O-dimethyltransferase
Sysname
2 S-adenosyl-L-methionine + trans-resveratrol = 2 S-adenosyl-L-homocysteine + pterostilbene (overall reaction) [RN:R09872];(1a) S-adenosyl-L-methionine + trans-resveratrol = S-adenosyl-L-homocysteine + 3-methoxy-4',5-dihydroxy-trans-stilbene [RN:R09870];(1b) S-adenosyl-L-methionine + 3-methoxy-4',5-dihydroxy-trans-stilbene = S-adenosyl-L-homocysteine + pterostilbene [RN:R09871]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];pterostilbene [CPD:C10287];3-methoxy-4',5-dihydroxy-trans-stilbene [CPD:C20154]
Product
Species information
Mikania micrantha
Species name
Bittervine (Mikania micrantha) is a species of eudicot in the family Asteraceae (daisy family).
Species info
Gene sequence info
Mikania_micrantha.ROMT.fa
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>rna-gnl|WGS:SZYD|E3N88_mrna04080
MAEMAMQYGDEQSRGLLQSQAHIWSHIFSFINSMTLKCAIELQIPDIINSHGGPMLLSELVEALSINKDKSHLVYRLMRMLVHSGFFVKQSVSITQGNQEEEGEGYLLTPASKLLLKDDPLCMRPFVLAILDPLLMDPWQHMSKWFQNDDINSFQTTHGKMIWDFAFQEPILNKLLNEAMATDAKLVPEVILKHCGGLFEGVDSIVDVGGGTGNLAKSIAEAFPNISCISFDLPHVIKGLDGSKNLTYVAGDMFEAIPKADAILMKWILHDWNDEECIKILTQCKKAIPSKNNGGKLIVMDMVVINDEPGNKTLETQLHFDMTMMTLVTGKERTEKEWAKLFVDAGFSDYKINPVLGFRSIIEVYP
>rna-gnl|WGS:SZYD|E3N88_mrna04081
MQDSDEQSRGLLQSQAHIWSHIFSFINSMTLKCAIELQIPDIINRHGTPMLLSELVEALSISKEKSNFLYRLMRMLVHSGFFVKQSVSITRGSDEEEEGYLLAPASKLFLKEDPLCMRPFVLAMLDPILMDPWQHMSKWFQNDDINSFQTTHGKMVWDFTFQEPVLNKLFNEAMATDARLVSQVLLKHYGGVFEGIDSIVDVGGGTGNLAKSIAEAFPNISCISFDLPHVVKGLDGSKNLTYVGGDMFEAIPKANAVMMKWILHDWNDEECIKILVRCKEAILSKDNGGKLIIMDMVVINDEPDNKTLETQLQYDMLMMTTVAGKERTEKEWAKLFSDAGFSNYKITPVLGLRSIIEVYP
>rna-gnl|WGS:SZYD|E3N88_mrna04077
MAMQNEDQSEILLESQTHIWNHIYTFIKSMSLKCAIQLQIPDIIDHHGSPMLLSELVEALPINQERAQSVYRLMRILVHCGFFVKQSVSTTGGNVKEEEGYMLAPASRLLLKENPLSLRPYLLCMFNPIMMDPWQDLSKWFQNDDVTPFHMTHGRSLYDLADQELNLNQFFNEAMACDAKLCMGVVLEKRTDIFQGLDSVVDVGGGTGEVAKVIAKAFPNTKCICFDLPHVVNGLVESNNLTYVGGDMFEAIPKAHTILLKWILHNWNDEECKKILTNCKEAIPSKEHGGKLVIIDIVLKVDQDDNKIFETQLFFDMVMMIYLTGRQRTEKDWAKLFFDSGFSDYKVTPILGLRSLIEVYP
>rna-gnl|WGS:SZYD|E3N88_mrna04079
MAISNCHSHIKVEGNKKVEMAMQAGDDEQSRCLLQSQAHIWSHIFSFINSMTLKCAIELQIPDIINRHGTPMLLSELVEALSISKEKSNFVYRSAYAHACSLWFLRETKWIDSIVDVGGGIGNLAKSISEAFPNISCISFDLPHVVKGLDGNKNLTYVGGDMFEAIPKASAVMMKAIPSKDDGGKLIIIDMVVINDEPDNKTLETQLRYDMLMMTILAGKERTEKEWAKLFSDAGFSNYKITPVLGLRMVGRGRGRHMGGGRRPNTRSNTNAPPIHGNEIPHQVENTEAAQNEL
>rna-gnl|WGS:SZYD|E3N88_mrna09259
MARMATQEGDEQSRCLLQSQAHIYSHVFGFINSMTLKCAIELQIPDIINSHGRPMMLSELVEALSINKEKSHFVYRLMRMLVHSGFFEKQSVSLTRGNDEEEEEGYLLTPASKLFLKEDPLCMRPFVLSMLDPTLMDPWQHMGKWFQTDDLNPFQTATGRSLWDFASQEPMLNKFFNEAMSTDARLVAKVILKDCRCLFEGVNSIVDVGGGTGNLAKSIAEAFPNISCICFDLPHVVKGLDGSKNFRYVAGDMFEAIPKADVILMKWILHDWNDEECIKILTRCKEAIPSKNNGGKVIIMDIVPRTILWETSKSQELYFPYPKSRTPCGRPLNSRTTLFEFPLSQEFFLKVENPFRVVPNHMRNPSTLKNQHMKPRTPPHLVV
>rna-gnl|WGS:SZYD|E3N88_mrna19523
MAHQNVDLPKELLDSQAHVWNHIFSFINSMSLKCAVELQIPDIIDRHGSPMLLSELVEALGLNHERTQFVYRLMRILVHSGFFVKQCVSAPGENDDEGGVGYLLTPASRLLLKDEPLSVRPHLLAMLDPMLLDTWQNMSKWFQNDDINPFYTTHERMVWDLAGQEPKLNEFFNEAMANDSRLVTGVILKDCRSVFEGLDSIVDVGGGTGTVAKAIAESFPDIRCISFDLPRVVHGLVGSKNLSYVGGDMFETIPNAHAVLLKPIFYHHAISTTIITVVALVVGRWLTNNNRKILEMDNTTKEDEDKVHQTLARGCRGTERSQRRERSEKEWAKLFFDSGFSNYKITPILGLRSLIENGMNQMKSSCNQIPLFFPPSIESSKQNQTEESSNSLTEEAQRNQTCSLFNNSYCGCVVLLCHEPMEVWCYESVSYGTNESEQGIEGRRGIDVRQSTKEESMIEAYNVEARGRIEASDMTLRFHRVSHGGGVYQLPNLIVGSITKENLYKAFSNGIKAGQARSITKENLYKAFSDGIKADQANTFISLAKCSSACCRQFIPGNLYAPYVLNGLKFSGLPNHKLVLKVGVPVMLLRNIDQKSGLCNGTRLQVISLGNHVIEAKIISGSNIGTCVFIPRLELTPSDKKIPFKFRRR
>rna-gnl|WGS:SZYD|E3N88_mrna19522
MAHQNVDMSKELLDSQAHVWNHIFSFINSMSLKCAVELQIPDIIDRHGSPMLLSELAEALGLNHERTQFVYRLMRVLVHSGFFVKQCVSAPEENDEEGGVGYLLTPASRLLLKDEPLNVRPFLLAMLDPITIDPWQNMSKWFQNDDINPFYTTHGRMVWDLAGQEPKLNLFFNEGMANDSRLVTSVILKDCRDIFEGLDSIVDVGGGTGTVAKAIAESFPDIMCINFDLPRVVNGLVGSKNLSYVGGDMFETIPNAHAVMLKWILHDWSDKECIKLLKQCKESIPSKENGGKLIIIDMVVKDHTSNNLLETQLFFDMLMMTLVTGRERSEKEWAKLFFESGFSNYKITPILGLRSLIEVYP
>rna-gnl|WGS:SZYD|E3N88_mrna19525
MALQNGDLSKQLLDSQAHVWNHIFSFINSMSLKCAVELQIPDIIDRHGSPMLLSELVEALGLNHERTQFVYRLMRILVHSGFFVKQCVSAPGENGEEGGVGYLLTPASRLLLKDEPLSVRPFLLAMLDPMLLDPWQNMSKWFQNDDINPFHTTHEKMLWDLAGHEPKLNRFFNEGMASDARLVTSVILKHCKGVFEGLDSIVDVGGGTGTVAKAIAESFPDIKCINFDFPHVVDGLIGSKNLSYVGGDMFEAIPNAHAVMLKWILHDWSDKECIKILKQCKESIPSKRNGGKLIIIDMVVKVHTDNNILETQLFFDMLMMTLVTGKERSEKEWAKLFFDSGFSNYKITPILGLRSLIEVYP
>rna-gnl|WGS:SZYD|E3N88_mrna19524
MCSHTCHIVSLKEKNLHMAHQNVDLSKELLNSQAHVWNHIFSFINSMSLKCAVELQIPDIIDRHGSPMLLSELVEALGLNHERTQFVYRLMRILVNSGFFVKQCVSAPGENDEEEGGVGYLLTPSSRLLLNDEPLSVRPFLLAMLDPITLNPWQNLSKWFQNDDINPFYTTHERMVWDVAGHEPKLNRFFNEAMANDSRLVTSVILKDCRDVFEGLDSIVDVGGGTGTVAKAIAESFPDIMCINFDLPRVVNGLVGSKNLSYVGGDMFETIPNAHAVMLKWILHDWSDKECIKLLKQCKESIPSKENGGKLIIIDMVVKDHTSNNLLETQLFFDMLMMTLLTGRERSEKEWAKLFFDSGFSSYKITPILGLRSLIEVYP
MAEMAMQYGDEQSRGLLQSQAHIWSHIFSFINSMTLKCAIELQIPDIINSHGGPMLLSELVEALSINKDKSHLVYRLMRMLVHSGFFVKQSVSITQGNQEEEGEGYLLTPASKLLLKDDPLCMRPFVLAILDPLLMDPWQHMSKWFQNDDINSFQTTHGKMIWDFAFQEPILNKLLNEAMATDAKLVPEVILKHCGGLFEGVDSIVDVGGGTGNLAKSIAEAFPNISCISFDLPHVIKGLDGSKNLTYVAGDMFEAIPKADAILMKWILHDWNDEECIKILTQCKKAIPSKNNGGKLIVMDMVVINDEPGNKTLETQLHFDMTMMTLVTGKERTEKEWAKLFVDAGFSDYKINPVLGFRSIIEVYP
>rna-gnl|WGS:SZYD|E3N88_mrna04081
MQDSDEQSRGLLQSQAHIWSHIFSFINSMTLKCAIELQIPDIINRHGTPMLLSELVEALSISKEKSNFLYRLMRMLVHSGFFVKQSVSITRGSDEEEEGYLLAPASKLFLKEDPLCMRPFVLAMLDPILMDPWQHMSKWFQNDDINSFQTTHGKMVWDFTFQEPVLNKLFNEAMATDARLVSQVLLKHYGGVFEGIDSIVDVGGGTGNLAKSIAEAFPNISCISFDLPHVVKGLDGSKNLTYVGGDMFEAIPKANAVMMKWILHDWNDEECIKILVRCKEAILSKDNGGKLIIMDMVVINDEPDNKTLETQLQYDMLMMTTVAGKERTEKEWAKLFSDAGFSNYKITPVLGLRSIIEVYP
>rna-gnl|WGS:SZYD|E3N88_mrna04077
MAMQNEDQSEILLESQTHIWNHIYTFIKSMSLKCAIQLQIPDIIDHHGSPMLLSELVEALPINQERAQSVYRLMRILVHCGFFVKQSVSTTGGNVKEEEGYMLAPASRLLLKENPLSLRPYLLCMFNPIMMDPWQDLSKWFQNDDVTPFHMTHGRSLYDLADQELNLNQFFNEAMACDAKLCMGVVLEKRTDIFQGLDSVVDVGGGTGEVAKVIAKAFPNTKCICFDLPHVVNGLVESNNLTYVGGDMFEAIPKAHTILLKWILHNWNDEECKKILTNCKEAIPSKEHGGKLVIIDIVLKVDQDDNKIFETQLFFDMVMMIYLTGRQRTEKDWAKLFFDSGFSDYKVTPILGLRSLIEVYP
>rna-gnl|WGS:SZYD|E3N88_mrna04079
MAISNCHSHIKVEGNKKVEMAMQAGDDEQSRCLLQSQAHIWSHIFSFINSMTLKCAIELQIPDIINRHGTPMLLSELVEALSISKEKSNFVYRSAYAHACSLWFLRETKWIDSIVDVGGGIGNLAKSISEAFPNISCISFDLPHVVKGLDGNKNLTYVGGDMFEAIPKASAVMMKAIPSKDDGGKLIIIDMVVINDEPDNKTLETQLRYDMLMMTILAGKERTEKEWAKLFSDAGFSNYKITPVLGLRMVGRGRGRHMGGGRRPNTRSNTNAPPIHGNEIPHQVENTEAAQNEL
>rna-gnl|WGS:SZYD|E3N88_mrna09259
MARMATQEGDEQSRCLLQSQAHIYSHVFGFINSMTLKCAIELQIPDIINSHGRPMMLSELVEALSINKEKSHFVYRLMRMLVHSGFFEKQSVSLTRGNDEEEEEGYLLTPASKLFLKEDPLCMRPFVLSMLDPTLMDPWQHMGKWFQTDDLNPFQTATGRSLWDFASQEPMLNKFFNEAMSTDARLVAKVILKDCRCLFEGVNSIVDVGGGTGNLAKSIAEAFPNISCICFDLPHVVKGLDGSKNFRYVAGDMFEAIPKADVILMKWILHDWNDEECIKILTRCKEAIPSKNNGGKVIIMDIVPRTILWETSKSQELYFPYPKSRTPCGRPLNSRTTLFEFPLSQEFFLKVENPFRVVPNHMRNPSTLKNQHMKPRTPPHLVV
>rna-gnl|WGS:SZYD|E3N88_mrna19523
MAHQNVDLPKELLDSQAHVWNHIFSFINSMSLKCAVELQIPDIIDRHGSPMLLSELVEALGLNHERTQFVYRLMRILVHSGFFVKQCVSAPGENDDEGGVGYLLTPASRLLLKDEPLSVRPHLLAMLDPMLLDTWQNMSKWFQNDDINPFYTTHERMVWDLAGQEPKLNEFFNEAMANDSRLVTGVILKDCRSVFEGLDSIVDVGGGTGTVAKAIAESFPDIRCISFDLPRVVHGLVGSKNLSYVGGDMFETIPNAHAVLLKPIFYHHAISTTIITVVALVVGRWLTNNNRKILEMDNTTKEDEDKVHQTLARGCRGTERSQRRERSEKEWAKLFFDSGFSNYKITPILGLRSLIENGMNQMKSSCNQIPLFFPPSIESSKQNQTEESSNSLTEEAQRNQTCSLFNNSYCGCVVLLCHEPMEVWCYESVSYGTNESEQGIEGRRGIDVRQSTKEESMIEAYNVEARGRIEASDMTLRFHRVSHGGGVYQLPNLIVGSITKENLYKAFSNGIKAGQARSITKENLYKAFSDGIKADQANTFISLAKCSSACCRQFIPGNLYAPYVLNGLKFSGLPNHKLVLKVGVPVMLLRNIDQKSGLCNGTRLQVISLGNHVIEAKIISGSNIGTCVFIPRLELTPSDKKIPFKFRRR
>rna-gnl|WGS:SZYD|E3N88_mrna19522
MAHQNVDMSKELLDSQAHVWNHIFSFINSMSLKCAVELQIPDIIDRHGSPMLLSELAEALGLNHERTQFVYRLMRVLVHSGFFVKQCVSAPEENDEEGGVGYLLTPASRLLLKDEPLNVRPFLLAMLDPITIDPWQNMSKWFQNDDINPFYTTHGRMVWDLAGQEPKLNLFFNEGMANDSRLVTSVILKDCRDIFEGLDSIVDVGGGTGTVAKAIAESFPDIMCINFDLPRVVNGLVGSKNLSYVGGDMFETIPNAHAVMLKWILHDWSDKECIKLLKQCKESIPSKENGGKLIIIDMVVKDHTSNNLLETQLFFDMLMMTLVTGRERSEKEWAKLFFESGFSNYKITPILGLRSLIEVYP
>rna-gnl|WGS:SZYD|E3N88_mrna19525
MALQNGDLSKQLLDSQAHVWNHIFSFINSMSLKCAVELQIPDIIDRHGSPMLLSELVEALGLNHERTQFVYRLMRILVHSGFFVKQCVSAPGENGEEGGVGYLLTPASRLLLKDEPLSVRPFLLAMLDPMLLDPWQNMSKWFQNDDINPFHTTHEKMLWDLAGHEPKLNRFFNEGMASDARLVTSVILKHCKGVFEGLDSIVDVGGGTGTVAKAIAESFPDIKCINFDFPHVVDGLIGSKNLSYVGGDMFEAIPNAHAVMLKWILHDWSDKECIKILKQCKESIPSKRNGGKLIIIDMVVKVHTDNNILETQLFFDMLMMTLVTGKERSEKEWAKLFFDSGFSNYKITPILGLRSLIEVYP
>rna-gnl|WGS:SZYD|E3N88_mrna19524
MCSHTCHIVSLKEKNLHMAHQNVDLSKELLNSQAHVWNHIFSFINSMSLKCAVELQIPDIIDRHGSPMLLSELVEALGLNHERTQFVYRLMRILVNSGFFVKQCVSAPGENDEEEGGVGYLLTPSSRLLLNDEPLSVRPFLLAMLDPITLNPWQNLSKWFQNDDINPFYTTHERMVWDVAGHEPKLNRFFNEAMANDSRLVTSVILKDCRDVFEGLDSIVDVGGGTGTVAKAIAESFPDIMCINFDLPRVVNGLVGSKNLSYVGGDMFETIPNAHAVMLKWILHDWSDKECIKLLKQCKESIPSKENGGKLIIIDMVVKDHTSNNLLETQLFFDMLMMTLLTGRERSEKEWAKLFFDSGFSSYKITPILGLRSLIEVYP
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University