Enzyme
4CL
Enzyme
4-coumarate:CoA ligase
Enzyme name
EC:6.2.1.12
Entry
Ligases;Forming carbon-sulfur bonds;Acid-thiol ligases
Class
4-coumarate:CoA ligase (AMP-forming)
Sysname
ATP + 4-coumarate + CoA = AMP + diphosphate + 4-coumaroyl-CoA [RN:R01616]
Reaction
AMP [CPD:C00020];diphosphate [CPD:C00013];4-coumaroyl-CoA [CPD:C00223]
Product
Species information
Mikania micrantha
Species name
Bittervine (Mikania micrantha) is a species of eudicot in the family Asteraceae (daisy family).
Species info
Gene sequence info
Mikania_micrantha.4CL.fa
Download
>rna-gnl|WGS:SZYD|E3N88_mrna26091
MEAKPEIIFRSKLPDIYIPKHLPLHSYCFENISSFSHRPCLIDGATGHEYTYAEVELTSRKVGSALYKHGISKGDVIMILLPNSSEFVFSFLGASYIGAMSTMANPFFTSAEIVKQAKASGAKIIVTQSVHVAKVKDFASENSIKVVSIDSTVDGCLHFSELISGDENELPVVDISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLWIHSEDVLICVLPLFHIYSLNSILLCGLRAGAAILIMQKFDIVPFLELIQRYKVTIGPFVPPIVLTIAKNEEIVDKYDLSSIRTVMSGAAPLGKELEDTVRSKFTNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGACGTVVRNAEMKIVDPETGASLPRNQRGEICIRGDQIMKGYLNDPESTKRTIVDGWLHTGDIGMIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPDISDAAVVPMIEENAGEVPVAFVVKSNGSTVTEAEIKQFISKQVVFYKRINRVFFVETIPKSPSGKILRKDLRAKLAAGVPN
>rna-gnl|WGS:SZYD|E3N88_mrna40686
MKRSFSEADTHVFPFPTTSRHVVVVVLPNVVEYAIVALGIMEAGGVFSGANPSSHSSEIKKQVDLAEAKLIVTNDHTYEKVKELGLPVIIVGEERAQGTIFYEELLEASDRAGNNGIESTVNQNDLCALPFSSGTTGLSKGVMLTHRNIVANLCSTLFSVGPDLIGKVTILGLIPYFHIYGLTGILCSTLRNKGKVVVMGRYDLPAVLKALIDHEVTFAPIVPPIVLGLVKHPIDEDLAKLKLRSVMTAAAPLAPEIYEEFHRKFPQIEVQEAYGMTEHSCITLTHGDPRKGHHTAKKRSVGYILPNLEVKFIDPDTGRSLPANTHGEICVRSQCVMKGYYKNEAETAQTIDEQGWLHTGDVGYIDDEGDVFIVDRMKELIKYKGFQVAPAELEGILLGHPSVEDAAVVGLPDKEAGEIPGAHVVISKDSKESEEDLMKYVANNVAHYKKVRVLHFVDKIPKSPSGKIMRRLIKENSVDTHPAPVSTTAAQPPAPHGAYTTHRDLQVQPWQLKRYYSTPVVTTRDEGSPDGLGRRRHQNNHPTSSVTCWWFGVSINSAKVVTTGLRPTTMVASNDDHVVQVVHPSRSANKNILRRLAEQWKEVSVKQSSLDILSETLEE
>rna-gnl|WGS:SZYD|E3N88_mrna08420
MVNLVRFCVSNRRVDSSTTRTLTAVASIIKHYHFNTNQNLKSPDQCAAVHGAARNIVAVFWDLDNKPPRLVSPFDAAVRLKKAAESFGAVRYKIAYANQHSFDYVPPEIRVHRRDRKTLNQLENKGVVKPLDPYICRVCGRKFYTNENLINHFKQIHEREHKKRVSQIESARGSQRLKLVGKYSMNMEKYNNAARDILTPKVGYGLGDDLKRAGYWVSVVSNKPQAADVALRNHMVDMMDRQQMECLILVSDDSDFVEVLKEARLRCLKTVVVGDNNDGALKRVSDAAFSWQEIITGKAKKEAVSVVGRWKDGVILKRLEWTYNYKRERKLYGSCTDDDDDDLNDDLVVDNLISDEVETDQSRAWWKLESDSDPADILQSWDNELEEAQTTKTPATTFLNHSIFYIKGWYPVSGYLHSQSIRLQVHLTCYELQLEGYQYRGIDMELYCANGDVEDRMALNNASTSQVIWLAIKPFFNNYSKSCVSMANFPKMQSNFVKSSSKGYNFEFSKNQSSSQDLYSDKTGVYISRHPQITLPTDPFLDVVSCVFSHKHDGKTALIDAASGVSISYSELQPMVKLMANGLHHQLGVSKGDVVLILLPNSIYYPIILLGVLYLGAVVTTMNPLSSFSEVKKQTLGLHASVAFTVLDRADELNSIGISSVIVPEIARFDSNHDQFAPFYKLISGESGDHDSVPPRTIRQDDPAAILYSSGTTGSSKGVVLSHRNLICGVELFVRFEASQYEFKPEENVYLAVVPMFHIYGLTLFTLGMLSLGTKIVVVKKFSLNEMVRAIDRYAVTHFPGVPPLVTALITAGKGVDGKSLKSLKQVSCGAAPLSTRIIEEFVHYFPHVDFIQGYGMSESTAVGTRGFNTRDVHNYASAGLLAPNMQAKVVDWVTGLHLPPGKAGELWLRGPAIMKEYFNNMEATSSTIDKDGWLHTGDIVYFDQDGYLYIVDRVKEIIKYKGFQIAPADLEDVLTSHPAVLDAAVTGIRDEEAGEVPVAFVVMRPGAEASESSIIDFVAKQVSPYKKVRRVMFTNKIPRSAAGKILRRQLKDQLSSRL
>rna-gnl|WGS:SZYD|E3N88_mrna08460
MANFPKMQSNFVKSSSEGYNFEFSKNQSSSHDLYSDETGVYISRHPQITLPTDSFLDVVSRVFSHKHDGKTALIDAASGVSISYSELQPMVKLMANGLHHQLGVSKGDVVLILLPNSIYYPIILLGVLYLGAVVTTMNPLSSFSEVKKQTLGLHASVAFTVLDRADELNSIGISSVIVPEIARFDSNHDQFEPFYKLISGESGDHDSVPPRTIRQDDPAAILYSSGTTGSSKGVVLSHRNLICGVELFVRFEASQYEFKPEENVYLAVVPMFHIYGLTLFTLGMLSLGTKIVVVKKFSLNEMVRAIDRYAVTHFPGVPPLVTALITAGKGVDGKSLKSLKQVSCGAAPLSTRIIEEFVHYFPHVDFIQGYGMSESTAVGTRGFNTRDVHHYASAGLLAPNMQAKVVDWVTGLHLPPGKAGELWLRGPAIMKEYFNNVEATSSTIDKDGWLHTGDIVYFDHDGYLYIVDRVKEIIKYKGFQIAPADLEDVLASHPAVLDAAVTGIRDEEAGEVPVAFVVMRPGAEASESSIIDFVAKQVSPYKKVRRVMFTNKIPRSAAGKILRRQLKDQLSSRL
>rna-gnl|WGS:SZYD|E3N88_mrna14461
MADSQTSLSVYNSGTGIYTSPRPPISLPPSPATSIVDFLFRNLRTHSDSPALIDPYSHKLITFSQLKQQISDLAIVLHHTFNISKNDVVLIFSPNSVLFPIAFFALTSIGAIVTVANPLYTVNELAHQISDSKPKLIITVNDLYPKVNGFNLPVVDLNNYSDLIEKSRHKTVTYPSISQNDVAVILYSSGTTGLSKGVVLTHRSIIATSLMVTADQEFYGEGRSVFLLVVPLFHVMGLITFTYSQLQRGNAMVVMARFELEKALDAIDRYRVTHLYTAPPVVVALVKQPEVVRRYDTSSLIEIGSGAAPLGKDVMDECSKIFPQTKVLQGYGMTETGGIISVENTRVGSRHSGSSGTLCPGMEAQIIHIETLKPLPPNQLGEIWVLINKYVYIFLTEYFHNKEATEETIDKQGWLHTGDLGYFDEEGRIYVVDRLKELIKYKGYQVAPAELEGLLMSHPEIMDAAVIPYTDEEANEIPMAYVVLKTGSTLTGKEVQEFIAKQERCPRRGEGHRWRGEEVVGENWRDGKEPFPFFFLI
>rna-gnl|WGS:SZYD|E3N88_mrna14491
MADSQTSLSVYNSGTGIYTSPRPPISLPPSPATSIVDFLFRNLRTHSDSPALIDPYSHKLITFSQLKQQISDLAIVLHHTFNISKNDVVLIFSPNSVLFPIAFFALTSIGAIVTVANPLYTVNELAHQISDSKPKLIITVNELYPKVNGFNLPVVDLNNYSDLIEKSRHKTVTYPSISQNDVAVILYSSGTTGLSKGVVLTHRSIIATSLMVTADQEFYGEGRSVFLLVVPLFHVMGLITFTYSQLQRGNAMVVMARFELEKALDAIDRYRVTHLYTAPPVVVALAKQPEVVRRYDTSSLIEIGSGAAPLGKDVMDECSKIFPQTKVLQGYGMTETGGIISVENTRVGSRHSGSSGTLCPGMEAQIIHIETLKPLPPNQLGEIWVLINKYVYIFLTEYFHNKEATEETIDKQGWLHTGDLGYFDEEGRIYVVDRLKELIKYKGYQVAPAELEGLLMSHPEIMDAAVIPYTDEEANEIPMAYVVLKTGSTLTGKEVQEFIAKQERCPRRGEGHRWRGEEVVGENWRDGKEPFPFFFLI
>rna-gnl|WGS:SZYD|E3N88_mrna16391
MEKSGYGRDGIFRSLRPPLIIPAKPNTSMVSFLFRNVSSYPNKTALIDSDSGETLTFSGLKTTVAKLSHALKTQLNITKNDVVLIFAPNSIQYPVAVYAIIALGAIVTTVNPQYTVGEISKQIKDSNPKVIITVQELYPKVENFGLPVVLLGSDCSKKGCFSYKDLISKSGSVTELPEVKIQGDDTAALLYSSGTTGVSKGVVLTHMNFISVSQMLTSDQRLMGEKDLVFLCFLPMFHIFGFGVVLYSQLQEGNTVVSMAKFDFVGMLKNVERYRVTHLWVVPPVFLGLAKQDVVSKFDLSSLKQIGSGAAPLGKELMEECAKKLPHVVVLQGYGMTETTGVISVESPIIGPRHSGSAGRLIPGVEAQIVSVDTNKPLPPNQMGEIWVRGANMMQGYFNNPQATKLTIDKQRFVHTGDLGYFDDEGQLFVVDRIKELIKYKGFQVAPAELEALLLSHSEILDAAVIPYPDAEAGEVPIAFVVRSPNSSLTEEDVKKFISEQVAPYKRLKRVTFVNSVPKSASGKLLRRELIEMVRSKM
>rna-gnl|WGS:SZYD|E3N88_mrna21253
MEPEKEIIFRSKLPDIYIPKHLPLHSYCFENIANFSHRPCLIDGATGQVYTYSDVELTSRKVASALHQHGISKGDVIMILLPNSPEFVYSFLGASYLGAVSTMANPFFTAAEIIKQAKASGAKIIVTQSSHVPKVHDYLKDNSIEIICIDSAPEGCSHFSELISGDETKLPQVEISSDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLWIHSEDGYGMTEAGPVLAMCLAFAKEPFEIKSGACGTVVRNAEMKIVDPESGVSLPRNQRGEICIRGDQIMKGYLNDPEATKRTIDSNGWLHTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPDISDAAVVPMVNEAAGEVPVAFVVKINGSSVTEDDIKQFVSKQVVFYKRINRVFFINTIPKSPSGKILRKELRAKLAAGVSN
MEAKPEIIFRSKLPDIYIPKHLPLHSYCFENISSFSHRPCLIDGATGHEYTYAEVELTSRKVGSALYKHGISKGDVIMILLPNSSEFVFSFLGASYIGAMSTMANPFFTSAEIVKQAKASGAKIIVTQSVHVAKVKDFASENSIKVVSIDSTVDGCLHFSELISGDENELPVVDISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLWIHSEDVLICVLPLFHIYSLNSILLCGLRAGAAILIMQKFDIVPFLELIQRYKVTIGPFVPPIVLTIAKNEEIVDKYDLSSIRTVMSGAAPLGKELEDTVRSKFTNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGACGTVVRNAEMKIVDPETGASLPRNQRGEICIRGDQIMKGYLNDPESTKRTIVDGWLHTGDIGMIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPDISDAAVVPMIEENAGEVPVAFVVKSNGSTVTEAEIKQFISKQVVFYKRINRVFFVETIPKSPSGKILRKDLRAKLAAGVPN
>rna-gnl|WGS:SZYD|E3N88_mrna40686
MKRSFSEADTHVFPFPTTSRHVVVVVLPNVVEYAIVALGIMEAGGVFSGANPSSHSSEIKKQVDLAEAKLIVTNDHTYEKVKELGLPVIIVGEERAQGTIFYEELLEASDRAGNNGIESTVNQNDLCALPFSSGTTGLSKGVMLTHRNIVANLCSTLFSVGPDLIGKVTILGLIPYFHIYGLTGILCSTLRNKGKVVVMGRYDLPAVLKALIDHEVTFAPIVPPIVLGLVKHPIDEDLAKLKLRSVMTAAAPLAPEIYEEFHRKFPQIEVQEAYGMTEHSCITLTHGDPRKGHHTAKKRSVGYILPNLEVKFIDPDTGRSLPANTHGEICVRSQCVMKGYYKNEAETAQTIDEQGWLHTGDVGYIDDEGDVFIVDRMKELIKYKGFQVAPAELEGILLGHPSVEDAAVVGLPDKEAGEIPGAHVVISKDSKESEEDLMKYVANNVAHYKKVRVLHFVDKIPKSPSGKIMRRLIKENSVDTHPAPVSTTAAQPPAPHGAYTTHRDLQVQPWQLKRYYSTPVVTTRDEGSPDGLGRRRHQNNHPTSSVTCWWFGVSINSAKVVTTGLRPTTMVASNDDHVVQVVHPSRSANKNILRRLAEQWKEVSVKQSSLDILSETLEE
>rna-gnl|WGS:SZYD|E3N88_mrna08420
MVNLVRFCVSNRRVDSSTTRTLTAVASIIKHYHFNTNQNLKSPDQCAAVHGAARNIVAVFWDLDNKPPRLVSPFDAAVRLKKAAESFGAVRYKIAYANQHSFDYVPPEIRVHRRDRKTLNQLENKGVVKPLDPYICRVCGRKFYTNENLINHFKQIHEREHKKRVSQIESARGSQRLKLVGKYSMNMEKYNNAARDILTPKVGYGLGDDLKRAGYWVSVVSNKPQAADVALRNHMVDMMDRQQMECLILVSDDSDFVEVLKEARLRCLKTVVVGDNNDGALKRVSDAAFSWQEIITGKAKKEAVSVVGRWKDGVILKRLEWTYNYKRERKLYGSCTDDDDDDLNDDLVVDNLISDEVETDQSRAWWKLESDSDPADILQSWDNELEEAQTTKTPATTFLNHSIFYIKGWYPVSGYLHSQSIRLQVHLTCYELQLEGYQYRGIDMELYCANGDVEDRMALNNASTSQVIWLAIKPFFNNYSKSCVSMANFPKMQSNFVKSSSKGYNFEFSKNQSSSQDLYSDKTGVYISRHPQITLPTDPFLDVVSCVFSHKHDGKTALIDAASGVSISYSELQPMVKLMANGLHHQLGVSKGDVVLILLPNSIYYPIILLGVLYLGAVVTTMNPLSSFSEVKKQTLGLHASVAFTVLDRADELNSIGISSVIVPEIARFDSNHDQFAPFYKLISGESGDHDSVPPRTIRQDDPAAILYSSGTTGSSKGVVLSHRNLICGVELFVRFEASQYEFKPEENVYLAVVPMFHIYGLTLFTLGMLSLGTKIVVVKKFSLNEMVRAIDRYAVTHFPGVPPLVTALITAGKGVDGKSLKSLKQVSCGAAPLSTRIIEEFVHYFPHVDFIQGYGMSESTAVGTRGFNTRDVHNYASAGLLAPNMQAKVVDWVTGLHLPPGKAGELWLRGPAIMKEYFNNMEATSSTIDKDGWLHTGDIVYFDQDGYLYIVDRVKEIIKYKGFQIAPADLEDVLTSHPAVLDAAVTGIRDEEAGEVPVAFVVMRPGAEASESSIIDFVAKQVSPYKKVRRVMFTNKIPRSAAGKILRRQLKDQLSSRL
>rna-gnl|WGS:SZYD|E3N88_mrna08460
MANFPKMQSNFVKSSSEGYNFEFSKNQSSSHDLYSDETGVYISRHPQITLPTDSFLDVVSRVFSHKHDGKTALIDAASGVSISYSELQPMVKLMANGLHHQLGVSKGDVVLILLPNSIYYPIILLGVLYLGAVVTTMNPLSSFSEVKKQTLGLHASVAFTVLDRADELNSIGISSVIVPEIARFDSNHDQFEPFYKLISGESGDHDSVPPRTIRQDDPAAILYSSGTTGSSKGVVLSHRNLICGVELFVRFEASQYEFKPEENVYLAVVPMFHIYGLTLFTLGMLSLGTKIVVVKKFSLNEMVRAIDRYAVTHFPGVPPLVTALITAGKGVDGKSLKSLKQVSCGAAPLSTRIIEEFVHYFPHVDFIQGYGMSESTAVGTRGFNTRDVHHYASAGLLAPNMQAKVVDWVTGLHLPPGKAGELWLRGPAIMKEYFNNVEATSSTIDKDGWLHTGDIVYFDHDGYLYIVDRVKEIIKYKGFQIAPADLEDVLASHPAVLDAAVTGIRDEEAGEVPVAFVVMRPGAEASESSIIDFVAKQVSPYKKVRRVMFTNKIPRSAAGKILRRQLKDQLSSRL
>rna-gnl|WGS:SZYD|E3N88_mrna14461
MADSQTSLSVYNSGTGIYTSPRPPISLPPSPATSIVDFLFRNLRTHSDSPALIDPYSHKLITFSQLKQQISDLAIVLHHTFNISKNDVVLIFSPNSVLFPIAFFALTSIGAIVTVANPLYTVNELAHQISDSKPKLIITVNDLYPKVNGFNLPVVDLNNYSDLIEKSRHKTVTYPSISQNDVAVILYSSGTTGLSKGVVLTHRSIIATSLMVTADQEFYGEGRSVFLLVVPLFHVMGLITFTYSQLQRGNAMVVMARFELEKALDAIDRYRVTHLYTAPPVVVALVKQPEVVRRYDTSSLIEIGSGAAPLGKDVMDECSKIFPQTKVLQGYGMTETGGIISVENTRVGSRHSGSSGTLCPGMEAQIIHIETLKPLPPNQLGEIWVLINKYVYIFLTEYFHNKEATEETIDKQGWLHTGDLGYFDEEGRIYVVDRLKELIKYKGYQVAPAELEGLLMSHPEIMDAAVIPYTDEEANEIPMAYVVLKTGSTLTGKEVQEFIAKQERCPRRGEGHRWRGEEVVGENWRDGKEPFPFFFLI
>rna-gnl|WGS:SZYD|E3N88_mrna14491
MADSQTSLSVYNSGTGIYTSPRPPISLPPSPATSIVDFLFRNLRTHSDSPALIDPYSHKLITFSQLKQQISDLAIVLHHTFNISKNDVVLIFSPNSVLFPIAFFALTSIGAIVTVANPLYTVNELAHQISDSKPKLIITVNELYPKVNGFNLPVVDLNNYSDLIEKSRHKTVTYPSISQNDVAVILYSSGTTGLSKGVVLTHRSIIATSLMVTADQEFYGEGRSVFLLVVPLFHVMGLITFTYSQLQRGNAMVVMARFELEKALDAIDRYRVTHLYTAPPVVVALAKQPEVVRRYDTSSLIEIGSGAAPLGKDVMDECSKIFPQTKVLQGYGMTETGGIISVENTRVGSRHSGSSGTLCPGMEAQIIHIETLKPLPPNQLGEIWVLINKYVYIFLTEYFHNKEATEETIDKQGWLHTGDLGYFDEEGRIYVVDRLKELIKYKGYQVAPAELEGLLMSHPEIMDAAVIPYTDEEANEIPMAYVVLKTGSTLTGKEVQEFIAKQERCPRRGEGHRWRGEEVVGENWRDGKEPFPFFFLI
>rna-gnl|WGS:SZYD|E3N88_mrna16391
MEKSGYGRDGIFRSLRPPLIIPAKPNTSMVSFLFRNVSSYPNKTALIDSDSGETLTFSGLKTTVAKLSHALKTQLNITKNDVVLIFAPNSIQYPVAVYAIIALGAIVTTVNPQYTVGEISKQIKDSNPKVIITVQELYPKVENFGLPVVLLGSDCSKKGCFSYKDLISKSGSVTELPEVKIQGDDTAALLYSSGTTGVSKGVVLTHMNFISVSQMLTSDQRLMGEKDLVFLCFLPMFHIFGFGVVLYSQLQEGNTVVSMAKFDFVGMLKNVERYRVTHLWVVPPVFLGLAKQDVVSKFDLSSLKQIGSGAAPLGKELMEECAKKLPHVVVLQGYGMTETTGVISVESPIIGPRHSGSAGRLIPGVEAQIVSVDTNKPLPPNQMGEIWVRGANMMQGYFNNPQATKLTIDKQRFVHTGDLGYFDDEGQLFVVDRIKELIKYKGFQVAPAELEALLLSHSEILDAAVIPYPDAEAGEVPIAFVVRSPNSSLTEEDVKKFISEQVAPYKRLKRVTFVNSVPKSASGKLLRRELIEMVRSKM
>rna-gnl|WGS:SZYD|E3N88_mrna21253
MEPEKEIIFRSKLPDIYIPKHLPLHSYCFENIANFSHRPCLIDGATGQVYTYSDVELTSRKVASALHQHGISKGDVIMILLPNSPEFVYSFLGASYLGAVSTMANPFFTAAEIIKQAKASGAKIIVTQSSHVPKVHDYLKDNSIEIICIDSAPEGCSHFSELISGDETKLPQVEISSDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLWIHSEDGYGMTEAGPVLAMCLAFAKEPFEIKSGACGTVVRNAEMKIVDPESGVSLPRNQRGEICIRGDQIMKGYLNDPEATKRTIDSNGWLHTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPDISDAAVVPMVNEAAGEVPVAFVVKINGSSVTEDDIKQFVSKQVVFYKRINRVFFINTIPKSPSGKILRKELRAKLAAGVSN
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University