Enzyme
The enzyme participates in the flavonoid biosynthesis pathway found in plants. It catalyses the double reduction of anthocyanidins, producing (2R,3R)-flavan-3-ol monomers required for the formation of proanthocyanidins. While the enzyme from the legume Medicago truncatula (MtANR) can use both NADPH and NADH as reductant, that from the crucifer Arabidopsis thaliana (AtANR) uses only NADPH. Also, while the substrate preference of MtANR is cyanidin>pelargonidin>delphinidin, the reverse preference is found with AtANR. cf. EC 1.3.1.112, anthocyanidin reductase [(2S)-flavan-3-ol-forming].
ANR
Enzyme
Anthocyanidin reductase
Enzyme name
EC:1.3.1.77
Entry
Oxidoreductases;Acting on the CH-CH group of donors;With NAD+ or NADP+ as acceptor
Class
(2R,3R)-flavan-3-ol:NAD(P)+ 3,4-oxidoreductase
Sysname
a (2R,3R)-flavan-3-ol + 2 NAD(P)+ = an anthocyanidin with a 3-hydroxy group + 2 NAD(P)H + H+ [RN:R07351 R07352]
Reaction
anthocyanidin with a 3-hydroxy group [CPD:C21381];NADH [CPD:C00004];NADPH [CPD:C00005];H+ [CPD:C00080]
Product
Species information
Melastoma candium
Species name
Species info
Gene sequence info
Melastoma_candium.ANR.fa
Download
>Mecan_G26539.1
MVNRRAIGKFELSLNDPKKTEHLRSLEGAGERLQLFKANLLEEGSFDGIVEGCEGVFHTASPFYHDVKDPQAELLDPALKGNSECPELLCESSFHQTLEKIRSVLYNTLRFAIRTAWYVLSKTLAEAAAWKFVNEKGIDMVTVNPAMVICPLLQPTLNTSAAAIFYLIKDFFLNTLRRPTSSMAAILMPDVVVDETWFSDPEICRKSKAWYMLSKTLAEEAAWNFANEKGIDMVVINPEVVIGPLLQPTLNTSSAEILNLIDGKPIPNQDTYRISQSSSKAKNPRRIGNIRDMPSGWVNIRDVAFEAPSASGRFHSFRHGRRTYRNSVASQACQPWATDTKIEVEEPRDRVHIAAETEPRGNRGELEGEGIHMLLKNHFFPSRPLVRFRITAVGGASNSLSLRRHGPALFLALSSSDEKTLGKEEEEELVGGGRGDGGGYGGHGGRGGGGDGNGGEWDDAGERNRKEAMLVVAEAGRTLESLPKDLASALAGGKIPAAVASRYLEMEKSGFLRWLMQFGGFKERLLADDLFLAKVGIECGVGIFTKTAAEYDRRRENFFNELEIVFADVVMAIIADFMLVYLPAPTVALRPALATNAGALAKFFHNCPDNAFQIALPGTSYSFLQRLGAIARNGSKLFAVGTTSSLVGTAATNALINARKAVDKSSAGEVENVPILSTSAAYGVYMAISSNLRYQVLAGVVEQRILEPLLHRNKVMLSAICFAVRTGNTYLGSLLWVDYARWIGIQ
>Mecan_G28925.1
MFISSSAKTLVAGMIKMEGTGMRDVLVVDPETEFLESKTKTGDEWEIYKENVRPLKRGRKVEVLNLALRAHQDCQLRKELLEKRRGFIKAIDDYDGDDPLQPWIECIKWVQESFPAGGESSGVVVLYEQCARAFWDSERYKNDLRYLKVWLEYAEHCKDAEVIYAFLDENDIGKKHSLFYVSYARHMESKDKVKAANEILNRGISSNAQPIKKLEDAFRKFLARSLSRPPVDDEEEDCSVPNRSFGTDLTMGGNSRRSQRNYDQASDKQMAKRVPLGIYQDKNSDISSSHSPNDASKKGLGPWKDIGARIERNKENNPMPSKWTSYKKPSVVAAGREVVASSASIEVYVDEDCKQRSTKAVQGRAWTKRKGHSNEGGDAEEASPAQFSHKAPAAVTACPPFICSVGSAFPSKAKIQQANPWSVQEGFIFLLSALLRESLHNLELFFDDRVDGLIQIDLDGFLDGIPDPPTMEDIPPEANAVSPSESAGSWIDHIERMLMEEVEEDGDGGRDEACESFLADLIVDSQAEGGSGSGEVVDASSCDDKVGDSEEDLEDKATGGRGSDRGDDEDDDGEAAVDGDPVSKKKKRQLRNRDAAARSRERRKVYVKELEMKSKYLESECLRLGRMLQCVMAENQALRFSLQNGGSTHDASAAKQESAVLLLESLLLGSLLWSLGIVCLFNLPLSIQPVLDVLGNVRRIEPEHLAPRGAKIEKLRLFNAGSFLTSRKCKSSRTKIKSDLTAQPLNKTINHSNLTKINTTSCAKKHDNPQNLTPPGILCPKPTFRSRKSRGESKTFWEGVVMSSSATGTSRCAACRNQRRRCPTDCIFSPHFPSTDPERFDAVHRIYGASKVAKMLEEIPPEMRGPAANALHFEAKCRIRDPVYGCVGIISTLNEEIRDTGTELAKLRARVAFLASGAQERQLGQDYTIVDDHWTEQQQIGNVDRTGDGGGAEIYDGGNVETWEWDVRAAEETRVATRLLPNHSSIIIIIIWSHLFSTTLFLRTEQSREQVSRRMEKGRVCVTGAGGFLASWVVEVLLSQGYVVHGTARDPGDAKNAHLNKLAEESGKLKIFKADLLDYDSLRSAIDGCIGVLHVASPVPPTSVPNPELELLEPAVTGTLNVLKASATSKVRRVVYVSSVASVMMNPNLPKDRAMDEACWSDKEYCRKTGNWYCLSKTEAESQAFDYAKSNGLDLVSICPSLILGPLLQPTLNASSAVLVNLLKGMPDPAPNRIRHIVDVRDVAAAVILAYEKPEAEGRYICSSHTINTVNLVEKLRSLFPDYNYPKNFTVGEEWGYFSNERLKMLGWTCRPLEETLVDAIRNYQGARLLLGAGGYIASWVVKFLLSRGYRVHGTVRDPGDPKNAHLMNLEYAAANLKLFKADLLDYESISAAVSGCTGVLHVASPVPAVKVMNPEVELYEPAHTGTKNVLNACLQEKVKKVVVVSSVAAIVITEAWPKDLAMDETCWSDIEHCKSLEASDRVIHQWYCVAKTLAETEAWEFAKRTRLNLVTVCPSIVIGPLLQPTVNASSMYLLALLQGTDPGELGGMAFVDVRDTAEAILLAYENPEADGRYICSSHEMRNEAIVEMLKEKYPEFNYPKSFREVHSDLRLSSQKLQNLGWKYRPFEETLLDAVENYKQCGLLATKTGDI
>Mecan_G08554.1
PLQHCRTILCRIDAGAYATSKLPLLHILGSPCLVFPPLRPHPFLIPSLHSFTAPSLHHLNTTQKVPTLRNQKAKHLTESQPNKTCKETGKGKDLLLGSSHGGCDQERERSKSGNTVEGRRERERGREGEPLLAGPVNILLHPTVTWGHTTGGVSSHHPGFQVGPTVGKQSPGGGSHIKSIPTHLPDKGEANICPPPKGQCMSERQEGKGRVVRVLGYIASWLMKLLLLHGYSIRETVRNPGRPAEGIKYERTAGGEGEGCVRDRCVGLHRVLARQAVAPSWVFRPSDQDTKKTEHLLALDGAKERLHLFKAELLDQGAFDAVINGCDGVFHTASPVAFEAVDPQADLIEPALKGTLNVLSSCAKAPSVKRVVFTSSMAAVVINGRPLTSDTTIDETWFSDPKFCEESKAWYMLSKTLAEMAAVDFARKNKIDLVMLNPGLVIGPLLQPTMNFSVGVLFDIVKGSETYPNTSFRFVNVRDVAQAHVLALENPSASGRHCLVARVIQCLDVLDILRNFYPTMTLPEKCEATQPLAASYQVPSVYHVSREKAEGLGVKFTPVEESPGMASAGSSYTVCVTGASGYIASWLVKLLLQRGYSVRATVRNPGHFRALSAALPCVTSDEACKILTSPSLYDPRKTAHLMALDGARERLRLFKAELLEEGAFDSAVAGCDGVFHTASPVFFTAADPKTELIDPAVEGTLNVLRSCSKAPSIKRIVLTSSIAAVSFNGKPLTSDVLVDETWFSDPKFCEETKIWYILSKTLAERAAWEFAEENRLNMVTINPGLVIGPLLQPTVNATAEIIFNLVNGAKTFPNTCYRFVDVRDVANAHILAYENPSASGRYCLAGRAMHIQDVVKVLHDFFPTLNLPDNNCIPANNDPFSFRESNDDLPRMQTIQDIVNLLESCRCEDDKSLAQTYQVSKTRAEGLGVSFTPIEISIRDTVDSLKEKGLLEE
>Mecan_G21625.1
MNGAKERLQLVKADLMDEGSFDNAVMGCSGMFHMGSPVLNPSFDPKAGDLMEQAEIVEPAIQGSLNVLRSCKKNPQLRRVVLTSSSSTVRVREDLNPSVPLDETSWSSVELCERLKIWYALSKTLAEKAAWDFCIENKIDLIMVIPAFVIRPSLPPEFVLDCF
>Mecan_G15961.1
MFVKGSTGYLGSYLVECLLERGHSGHSVHATVRNPGRVDQQDEGARCSLGPLVGEGNGDILSRPHGRQTNKHHRPAVRGIVNLLKSCSKSKSAERVVLTSSISTITAKDDSGKWKSFVDKSCRISFDHVYTLLKTLTEEAAFECAQQNSLGLVSVISPTIACPFPTLAVPSGIQFFLSPITDNIAILFFTPGSNTLIRRRPEHQVMELAIGSGVEAVKHQKGFMEDAGFGVGLPAYVKGIKTELNEDGKVWTENLKKVITDSCDSRTTTNTTEVGCGEGTEEREEEKGKEEEEKDKDEAGDGEEGA
>Mecan_G09678.1
MAAGFIGSWLIMRLLEQGYTVRATVRDPSNMKKVKHLLDLPKAKTHMTLWKADLNDEGSFDEAIQGCKGVFHVATPMDFESKDPENEVIKPTIKGVLSIMKACVKAKVRRLVFTSSAGTVNVQPTQKPVYDENSWSDLDFVRSVKMTGWVRTEFLTMWMTWQASSPISNPLYLKNAGLLETDTLAEQAAWKFAEENNLDFISIIPVLVVGPFIMSSFPPSLITALSPITRNEAHYTIIKQGQFVHLDDLCESHIFLYEHPEAKGRYICSSHDANIHHIAKLLKDKFPEYNVPTEFEGIDENLEIVSFSSKKLTDMGFKFKYSLEDMFVGAVECCREKGLLPLSYEKKAC
>Mecan_G00214.1
MILDIFESKGQFVDGVKFSGGSYSLMPKSFIQEITAIAHEHDVYVSTGDWAEHVIRQGPSSFKDFIQECKQLGFDTIELNAGSLEVPENSLLRFVRLAKGDGLKVRPLFAVKFNKSDIPSAGDRAYGAYVVPEPRSSEYVEDVDLLIRRAERCLEAGADMIMIDPDDICGAGAAIRSDIIAKLISRLGLARTMFEASNAATSEWFIQRYGPKVIRKDTSMASDRRKACVTGGSGYVASTLVKLLLERGFSVNTTVRNPDNEKKVLHLNELKRLGDLKVFRADLTDDASFDAPIDGCEFVFHVATPINFASTNPEKDVIEPAVQGVLNVLGACKRSKSVKRVVFTSSAAAVCGNNLHGTGLVMDENNWTDVDHLVSEKPPSWGYSVSKTLAEKAAWKFAKENGIDLVTVAPPYLAGSSIIPEVPGSLHIAMAPVTGNVFLINNLKNMQIKSGFIGLTHVEDACRAHIFVAEKGSASGRYNCCNVNTSLPKFAEFLKKRYPHYNISTEFSDVPYEAKTIMSSEKLIREGFAFKYSLEDICDQSINVLKEKGILQN
MVNRRAIGKFELSLNDPKKTEHLRSLEGAGERLQLFKANLLEEGSFDGIVEGCEGVFHTASPFYHDVKDPQAELLDPALKGNSECPELLCESSFHQTLEKIRSVLYNTLRFAIRTAWYVLSKTLAEAAAWKFVNEKGIDMVTVNPAMVICPLLQPTLNTSAAAIFYLIKDFFLNTLRRPTSSMAAILMPDVVVDETWFSDPEICRKSKAWYMLSKTLAEEAAWNFANEKGIDMVVINPEVVIGPLLQPTLNTSSAEILNLIDGKPIPNQDTYRISQSSSKAKNPRRIGNIRDMPSGWVNIRDVAFEAPSASGRFHSFRHGRRTYRNSVASQACQPWATDTKIEVEEPRDRVHIAAETEPRGNRGELEGEGIHMLLKNHFFPSRPLVRFRITAVGGASNSLSLRRHGPALFLALSSSDEKTLGKEEEEELVGGGRGDGGGYGGHGGRGGGGDGNGGEWDDAGERNRKEAMLVVAEAGRTLESLPKDLASALAGGKIPAAVASRYLEMEKSGFLRWLMQFGGFKERLLADDLFLAKVGIECGVGIFTKTAAEYDRRRENFFNELEIVFADVVMAIIADFMLVYLPAPTVALRPALATNAGALAKFFHNCPDNAFQIALPGTSYSFLQRLGAIARNGSKLFAVGTTSSLVGTAATNALINARKAVDKSSAGEVENVPILSTSAAYGVYMAISSNLRYQVLAGVVEQRILEPLLHRNKVMLSAICFAVRTGNTYLGSLLWVDYARWIGIQ
>Mecan_G28925.1
MFISSSAKTLVAGMIKMEGTGMRDVLVVDPETEFLESKTKTGDEWEIYKENVRPLKRGRKVEVLNLALRAHQDCQLRKELLEKRRGFIKAIDDYDGDDPLQPWIECIKWVQESFPAGGESSGVVVLYEQCARAFWDSERYKNDLRYLKVWLEYAEHCKDAEVIYAFLDENDIGKKHSLFYVSYARHMESKDKVKAANEILNRGISSNAQPIKKLEDAFRKFLARSLSRPPVDDEEEDCSVPNRSFGTDLTMGGNSRRSQRNYDQASDKQMAKRVPLGIYQDKNSDISSSHSPNDASKKGLGPWKDIGARIERNKENNPMPSKWTSYKKPSVVAAGREVVASSASIEVYVDEDCKQRSTKAVQGRAWTKRKGHSNEGGDAEEASPAQFSHKAPAAVTACPPFICSVGSAFPSKAKIQQANPWSVQEGFIFLLSALLRESLHNLELFFDDRVDGLIQIDLDGFLDGIPDPPTMEDIPPEANAVSPSESAGSWIDHIERMLMEEVEEDGDGGRDEACESFLADLIVDSQAEGGSGSGEVVDASSCDDKVGDSEEDLEDKATGGRGSDRGDDEDDDGEAAVDGDPVSKKKKRQLRNRDAAARSRERRKVYVKELEMKSKYLESECLRLGRMLQCVMAENQALRFSLQNGGSTHDASAAKQESAVLLLESLLLGSLLWSLGIVCLFNLPLSIQPVLDVLGNVRRIEPEHLAPRGAKIEKLRLFNAGSFLTSRKCKSSRTKIKSDLTAQPLNKTINHSNLTKINTTSCAKKHDNPQNLTPPGILCPKPTFRSRKSRGESKTFWEGVVMSSSATGTSRCAACRNQRRRCPTDCIFSPHFPSTDPERFDAVHRIYGASKVAKMLEEIPPEMRGPAANALHFEAKCRIRDPVYGCVGIISTLNEEIRDTGTELAKLRARVAFLASGAQERQLGQDYTIVDDHWTEQQQIGNVDRTGDGGGAEIYDGGNVETWEWDVRAAEETRVATRLLPNHSSIIIIIIWSHLFSTTLFLRTEQSREQVSRRMEKGRVCVTGAGGFLASWVVEVLLSQGYVVHGTARDPGDAKNAHLNKLAEESGKLKIFKADLLDYDSLRSAIDGCIGVLHVASPVPPTSVPNPELELLEPAVTGTLNVLKASATSKVRRVVYVSSVASVMMNPNLPKDRAMDEACWSDKEYCRKTGNWYCLSKTEAESQAFDYAKSNGLDLVSICPSLILGPLLQPTLNASSAVLVNLLKGMPDPAPNRIRHIVDVRDVAAAVILAYEKPEAEGRYICSSHTINTVNLVEKLRSLFPDYNYPKNFTVGEEWGYFSNERLKMLGWTCRPLEETLVDAIRNYQGARLLLGAGGYIASWVVKFLLSRGYRVHGTVRDPGDPKNAHLMNLEYAAANLKLFKADLLDYESISAAVSGCTGVLHVASPVPAVKVMNPEVELYEPAHTGTKNVLNACLQEKVKKVVVVSSVAAIVITEAWPKDLAMDETCWSDIEHCKSLEASDRVIHQWYCVAKTLAETEAWEFAKRTRLNLVTVCPSIVIGPLLQPTVNASSMYLLALLQGTDPGELGGMAFVDVRDTAEAILLAYENPEADGRYICSSHEMRNEAIVEMLKEKYPEFNYPKSFREVHSDLRLSSQKLQNLGWKYRPFEETLLDAVENYKQCGLLATKTGDI
>Mecan_G08554.1
PLQHCRTILCRIDAGAYATSKLPLLHILGSPCLVFPPLRPHPFLIPSLHSFTAPSLHHLNTTQKVPTLRNQKAKHLTESQPNKTCKETGKGKDLLLGSSHGGCDQERERSKSGNTVEGRRERERGREGEPLLAGPVNILLHPTVTWGHTTGGVSSHHPGFQVGPTVGKQSPGGGSHIKSIPTHLPDKGEANICPPPKGQCMSERQEGKGRVVRVLGYIASWLMKLLLLHGYSIRETVRNPGRPAEGIKYERTAGGEGEGCVRDRCVGLHRVLARQAVAPSWVFRPSDQDTKKTEHLLALDGAKERLHLFKAELLDQGAFDAVINGCDGVFHTASPVAFEAVDPQADLIEPALKGTLNVLSSCAKAPSVKRVVFTSSMAAVVINGRPLTSDTTIDETWFSDPKFCEESKAWYMLSKTLAEMAAVDFARKNKIDLVMLNPGLVIGPLLQPTMNFSVGVLFDIVKGSETYPNTSFRFVNVRDVAQAHVLALENPSASGRHCLVARVIQCLDVLDILRNFYPTMTLPEKCEATQPLAASYQVPSVYHVSREKAEGLGVKFTPVEESPGMASAGSSYTVCVTGASGYIASWLVKLLLQRGYSVRATVRNPGHFRALSAALPCVTSDEACKILTSPSLYDPRKTAHLMALDGARERLRLFKAELLEEGAFDSAVAGCDGVFHTASPVFFTAADPKTELIDPAVEGTLNVLRSCSKAPSIKRIVLTSSIAAVSFNGKPLTSDVLVDETWFSDPKFCEETKIWYILSKTLAERAAWEFAEENRLNMVTINPGLVIGPLLQPTVNATAEIIFNLVNGAKTFPNTCYRFVDVRDVANAHILAYENPSASGRYCLAGRAMHIQDVVKVLHDFFPTLNLPDNNCIPANNDPFSFRESNDDLPRMQTIQDIVNLLESCRCEDDKSLAQTYQVSKTRAEGLGVSFTPIEISIRDTVDSLKEKGLLEE
>Mecan_G21625.1
MNGAKERLQLVKADLMDEGSFDNAVMGCSGMFHMGSPVLNPSFDPKAGDLMEQAEIVEPAIQGSLNVLRSCKKNPQLRRVVLTSSSSTVRVREDLNPSVPLDETSWSSVELCERLKIWYALSKTLAEKAAWDFCIENKIDLIMVIPAFVIRPSLPPEFVLDCF
>Mecan_G15961.1
MFVKGSTGYLGSYLVECLLERGHSGHSVHATVRNPGRVDQQDEGARCSLGPLVGEGNGDILSRPHGRQTNKHHRPAVRGIVNLLKSCSKSKSAERVVLTSSISTITAKDDSGKWKSFVDKSCRISFDHVYTLLKTLTEEAAFECAQQNSLGLVSVISPTIACPFPTLAVPSGIQFFLSPITDNIAILFFTPGSNTLIRRRPEHQVMELAIGSGVEAVKHQKGFMEDAGFGVGLPAYVKGIKTELNEDGKVWTENLKKVITDSCDSRTTTNTTEVGCGEGTEEREEEKGKEEEEKDKDEAGDGEEGA
>Mecan_G09678.1
MAAGFIGSWLIMRLLEQGYTVRATVRDPSNMKKVKHLLDLPKAKTHMTLWKADLNDEGSFDEAIQGCKGVFHVATPMDFESKDPENEVIKPTIKGVLSIMKACVKAKVRRLVFTSSAGTVNVQPTQKPVYDENSWSDLDFVRSVKMTGWVRTEFLTMWMTWQASSPISNPLYLKNAGLLETDTLAEQAAWKFAEENNLDFISIIPVLVVGPFIMSSFPPSLITALSPITRNEAHYTIIKQGQFVHLDDLCESHIFLYEHPEAKGRYICSSHDANIHHIAKLLKDKFPEYNVPTEFEGIDENLEIVSFSSKKLTDMGFKFKYSLEDMFVGAVECCREKGLLPLSYEKKAC
>Mecan_G00214.1
MILDIFESKGQFVDGVKFSGGSYSLMPKSFIQEITAIAHEHDVYVSTGDWAEHVIRQGPSSFKDFIQECKQLGFDTIELNAGSLEVPENSLLRFVRLAKGDGLKVRPLFAVKFNKSDIPSAGDRAYGAYVVPEPRSSEYVEDVDLLIRRAERCLEAGADMIMIDPDDICGAGAAIRSDIIAKLISRLGLARTMFEASNAATSEWFIQRYGPKVIRKDTSMASDRRKACVTGGSGYVASTLVKLLLERGFSVNTTVRNPDNEKKVLHLNELKRLGDLKVFRADLTDDASFDAPIDGCEFVFHVATPINFASTNPEKDVIEPAVQGVLNVLGACKRSKSVKRVVFTSSAAAVCGNNLHGTGLVMDENNWTDVDHLVSEKPPSWGYSVSKTLAEKAAWKFAKENGIDLVTVAPPYLAGSSIIPEVPGSLHIAMAPVTGNVFLINNLKNMQIKSGFIGLTHVEDACRAHIFVAEKGSASGRYNCCNVNTSLPKFAEFLKKRYPHYNISTEFSDVPYEAKTIMSSEKLIREGFAFKYSLEDICDQSINVLKEKGILQN
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University