Enzyme
Requires Mg2+ for activity. The enzyme catalyses the methylation of daidzein and genistein. It does not methylate naringenin, apigenin, luteolin or kaempferol.
HI4'OMT
Enzyme
Isoflavone 4'-O-methyltransferase
Enzyme name
EC:2.1.1.46
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:4'-hydroxyisoflavone 4'-O-methyltransferase
Sysname
S-adenosyl-L-methionine + a 4'-hydroxyisoflavone = S-adenosyl-L-homocysteine + a 4'-methoxyisoflavone [RN:R07331]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];4'-methoxyisoflavone [CPD:C03074]
Product
Species information
Melaleuca alternifolia
Species name
Tea tree (Melaleuca alternifolia) is a species of eudicot in the family Myrtaceae (myrtle family).
Species info
Gene sequence info
Melaleuca_alternifolia.HI4_OMT.fa
Download
>scf7180000028461_mRNA_18
MVHGGPSWELMKGNPEVSDLFCGAMEHDSRLMTKVVLDTCGGVFKGLSSVVDVGGGSGTMGKAIAEAFPHLECTVLDLPHVVDGLVGSERLKFVGGDMFVEIPQADAILFKWILHDWSDEKCIKILKRSKEAVSGAVEVGKVIIIDMVVENRSGDHRSSETQLFFDMAMMVSVNAKERDEKEWKQLFVDAGFSGYKIISRLGLRSLIEVYP
>scf7180000028461_mRNA_23
MDLANRDGNVTASELLRAQDYIWNHMLSSVNSLCLKCVIQLGIPELIHDHGRPMTLLELVSALHIHPTKAHCIQRIMRILVHSGFFELRHLKEVDHQTGYALTVASRLLLKHNPSSLVPFVLVALHPFTMAPWQYLSTWLRDGDQDLTRSTLEMAHGGPAWELMRGNPELDNLFYEMMENDSRLMAKVVLDTCGGVFEGLSSVVDVGGGTGIMGKAITDAFPHLECTVFDLPHLVNGLVGSERLKFVGGDMFVEIPPANALLLKLILHDWNDEKCIEILKRSKEALSGGGKAGKVIVIDMVVGNQQEDHKSTETQLFFDVAMMVGFNGKERSEKEWEKLFVDAGFSSYKIISRLGLRSLIEIYP
>scf7180000029144_mRNA_106
MAHGVPSWEFARRNPEVNGLFNEAMARDSSLMAKAVVDTCGRVFEGLSSVVDVGGGTGTMGKVITEAFPHLECTVLDQPHVVDGLVGSDKLKFVGGDMFVEIPPADAIILKWILHDWSDEKCIEILKRSKEAVSGQGKARKAIMIDMVVGNQTEDHTSTESQLFFDMLMMVTFNGNERNEKEWEKLFVDVGFSNYKIVSRLGLRSLVEIYPSLCFCLFDLLV
>scf7180000029166_mRNA_27
MAHGVPSWEFARRNPEVNGLFNEAMARDSSLMAKAVVDTCGRVFEGLSSVVDVGGGTGTMGKVITEAFPHLECTVLDQPHVVDGLVGSDKLKFVGGDMFVEIPPADAIILKWILHDWSDEKCIEILKRSKEAVSGQGKARKAIMIDMVVGNQTEDHTSTESQLFFDMLMMVTFNGNERNEKEWEKLFVDVGFSNYKIVSRLGLRSLVEIYPSLCFCLFDLLV
>scf7180000029926_mRNA_67
MHHLSHSHEAKEMFHAQSHLYNHTFNFISSMSLKCAVQLCIPDVIHNHKQPMSLLELASALQIHPSKSTCLYRLMHVLVHSGIFAETLVPKNGEEEEKIKAYVLTPSSRLLLSDKVVGVSPFVAAMLDPALVTPWHFLGDWLQGNNEKDCRDKLTNPFEAAHGVDLWDYGRKNPEFGSIFNRAMASDSQMMNLVIQDCKEIFEGLNSLVDVGGGTGIVARIISEACPGLKCKVFDLPQVVAGLPESENVKFVDGDMFESIPPADAILLKFIVGGANLHRQQCEKSPMDSY
>scf7180000036317_mRNA_393
MHHLSHSHEAKEMFHAQSHLYNHTFNFISSMSLKCAVQLCIPDVIHNHKQPMSLLELASALQIHPSKSTCLYRLMHVLVHSGIFAETLVPKNGEEEEKIKAYVLTPSSRLLLSDKVVGVSPFVAAMLDPALVTPWHFLGDWLQGNNEKDCRDKLTNPFEAAHGVDLWDYGRKNPEFGSIFNRAMASDSQMMNLVIQDCKEIFEGLNSLVDVGGGTGIVARIISEACPGLKCKVFDLPQVVAGLPESENVKFVDGDMFESIPPADAILLKFIVGGANLHRQQCEKSPMDSY
MVHGGPSWELMKGNPEVSDLFCGAMEHDSRLMTKVVLDTCGGVFKGLSSVVDVGGGSGTMGKAIAEAFPHLECTVLDLPHVVDGLVGSERLKFVGGDMFVEIPQADAILFKWILHDWSDEKCIKILKRSKEAVSGAVEVGKVIIIDMVVENRSGDHRSSETQLFFDMAMMVSVNAKERDEKEWKQLFVDAGFSGYKIISRLGLRSLIEVYP
>scf7180000028461_mRNA_23
MDLANRDGNVTASELLRAQDYIWNHMLSSVNSLCLKCVIQLGIPELIHDHGRPMTLLELVSALHIHPTKAHCIQRIMRILVHSGFFELRHLKEVDHQTGYALTVASRLLLKHNPSSLVPFVLVALHPFTMAPWQYLSTWLRDGDQDLTRSTLEMAHGGPAWELMRGNPELDNLFYEMMENDSRLMAKVVLDTCGGVFEGLSSVVDVGGGTGIMGKAITDAFPHLECTVFDLPHLVNGLVGSERLKFVGGDMFVEIPPANALLLKLILHDWNDEKCIEILKRSKEALSGGGKAGKVIVIDMVVGNQQEDHKSTETQLFFDVAMMVGFNGKERSEKEWEKLFVDAGFSSYKIISRLGLRSLIEIYP
>scf7180000029144_mRNA_106
MAHGVPSWEFARRNPEVNGLFNEAMARDSSLMAKAVVDTCGRVFEGLSSVVDVGGGTGTMGKVITEAFPHLECTVLDQPHVVDGLVGSDKLKFVGGDMFVEIPPADAIILKWILHDWSDEKCIEILKRSKEAVSGQGKARKAIMIDMVVGNQTEDHTSTESQLFFDMLMMVTFNGNERNEKEWEKLFVDVGFSNYKIVSRLGLRSLVEIYPSLCFCLFDLLV
>scf7180000029166_mRNA_27
MAHGVPSWEFARRNPEVNGLFNEAMARDSSLMAKAVVDTCGRVFEGLSSVVDVGGGTGTMGKVITEAFPHLECTVLDQPHVVDGLVGSDKLKFVGGDMFVEIPPADAIILKWILHDWSDEKCIEILKRSKEAVSGQGKARKAIMIDMVVGNQTEDHTSTESQLFFDMLMMVTFNGNERNEKEWEKLFVDVGFSNYKIVSRLGLRSLVEIYPSLCFCLFDLLV
>scf7180000029926_mRNA_67
MHHLSHSHEAKEMFHAQSHLYNHTFNFISSMSLKCAVQLCIPDVIHNHKQPMSLLELASALQIHPSKSTCLYRLMHVLVHSGIFAETLVPKNGEEEEKIKAYVLTPSSRLLLSDKVVGVSPFVAAMLDPALVTPWHFLGDWLQGNNEKDCRDKLTNPFEAAHGVDLWDYGRKNPEFGSIFNRAMASDSQMMNLVIQDCKEIFEGLNSLVDVGGGTGIVARIISEACPGLKCKVFDLPQVVAGLPESENVKFVDGDMFESIPPADAILLKFIVGGANLHRQQCEKSPMDSY
>scf7180000036317_mRNA_393
MHHLSHSHEAKEMFHAQSHLYNHTFNFISSMSLKCAVQLCIPDVIHNHKQPMSLLELASALQIHPSKSTCLYRLMHVLVHSGIFAETLVPKNGEEEEKIKAYVLTPSSRLLLSDKVVGVSPFVAAMLDPALVTPWHFLGDWLQGNNEKDCRDKLTNPFEAAHGVDLWDYGRKNPEFGSIFNRAMASDSQMMNLVIQDCKEIFEGLNSLVDVGGGTGIVARIISEACPGLKCKVFDLPQVVAGLPESENVKFVDGDMFESIPPADAILLKFIVGGANLHRQQCEKSPMDSY
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University