Enzyme
Specific for quercetin. Related enzymes bring about the 3-O-methylation of other flavonols, such as galangin and kaempferol.
COMT
Enzyme
Flavone 3'-O-methyltransferase
Enzyme name
EC:2.1.1.76
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:3,5,7,3',4'-pentahydroxyflavone 3-O-methyltransferase
Sysname
S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone = S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone [RN:R02157]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];3-methoxy-5,7,3',4'-tetrahydroxyflavone [CPD:C04443]
Product
Species information
Megadenia pygmaea
Species name
Megadenia pygmaea is a species of eudicot in the family Brassicaceae (mustard family).
Species info
Gene sequence info
Megadenia_pygmaea.COMT.fa
Download
>Mpyg02345.t1
MGYLFEETLSSNPKTPVVTDDDNELGLMAVRLANAAAFPMVLKAALELGVFDTLHAASEFLSPSEIASRLPTTPRNPEAPVLLDRMLRLLASYSMVKCGKVSEGKGERVYKAEPICRFFLKNNIQDIGSLASQVIVNFDSVFLNTWAQLKDVVLEGGDAFGRAHGGMKLFDYMGTDERFSKLFNQTGFTIAVVKKALQVYEGFKGVNVLVDVGGGVGNTLGVVTSKYPNIKGINFDLTCALAQAPSIHGVEHVAGDMFVDVPTGDAMILKRILHDWTDEDCVKILKNCWKSLPENGKVIVIELVTPDEAENGDINANIAFDMDMLMFTQCSGGKERSRAEFEALAAASGFTRCKFVCPAYHCWIIEFIK
>Mpyg01407.t1
MEEQNLSLYIMILSSSSVLPMVLKTAIDLGLFDILAESAPSPLSASQIVSLLYTQTQQDHDSSLVNRILRCLASYSIVTCSVSTDHGEPRAVYGLAPVAKYFTKNQDGCGSLAPLVNLFQDKVVTDIWYNLKDSVLEGGLPFHKTHGSSAVELVGRDSRFREVFQSSMRGFNEVFMKEFLKNYNGFDGVKSLVDVGGGDGSILSEIISKHPHIIKAINFDLPSVINTSSASPGIGHVSGDMFTNIPKGEAIFMKWMLHSWDDENCVKILRNCYQSLPSNGKVIVVDMVVPDLPRDSLLDRSLFQFELFMMNMNPSGKERTKREFEILARLAGFSSVQVPFTALCFSVVEFHKNV
>Mpyg02344.t1
MGYLSEETLSSNLKTPVVTDDDNELGLMAVRLANAAAFPMVLKAALELGVFDTLHAASEFLSPSEIASRLPTTPRNPEAPILLDRMLRLLASYSMVKCGKVSEGKGERVYKAEPICRFFLKNNIQDIGSLASQVIVNFDSVFLNTWAQLKDVVLEGGDAFGRAHGGMKLFDYMGTDERFSKLFNQTGFTIAVVKKALEVYEGFKGVNVLVDVGGGVGNTLAVVTSKYPNIKGINFDLTCALAQAPSIPGVEHVAGDMFVDVPTGDAMILKRILHDWTDEDCVKILKNCWKSLPENGKVVVIELVTPDDAENGDINANIAFDMDMLMFTQCSGGKERSRAEFEALAAESGFTHCKFVCPAYHCWIIEFIK
>Mpyg09481.t1
MYSTAETQITPVQVTDDETSLFAMQLASCSVLPMVLQTALELDLLEIMAKNASQMSPAEITSHLPTKNPEAPVILDRILRLLAAYSILTCSVRTLPGGVGLDRLYGLGPVCKYLTKNEDGVSIAALCLMTQDKVFMESWYHLKDAILDGGSPFNKAYGMSVLEYQKTDARFNKVVTDGMSNHSTISMKKILETYKGFDGLTSLVDVGGGNGATLKMIVSKYPNLKGVNFDLPHVIKNAPSYSGIEHVGGDMFVSVPKGDAIFMKWICHARSDEQCFKFLKNCYDALPENGKVIVAECILPETLDSSVLTKQALHVDCIMLAHSRGGIERTEKEYEALAKGSGFHGIKVVCNAFGIYIIEFLKKN
>Mpyg08257.t1
MGSLAETQITPAKISDEEANLFAMQLAGATVLPMVLTSALELDLLEIISKNAGLAGGQLSPSEIASHLPTKNPDAPIMVDRILRLLAAYSILTCSVRKLLDGGVERLYGLGPVCKYLTKNEDGVSLAALCHLNRDRVFMESWYHLKDAVLEGGVPFDKAFGMDAFEYQGTDPRFNKVFNNGMSNHTTIVMTKILETYKGFEELSSLVDVGGGIGVTLRMIVSKHPHIKGILYDLSHVIEEAVSYPGIEHIGGDMFVNVPKADAIFMKWILHDWSDQHCLKVLKNCYEALPDNGKVIVAESILPVVPDSSLLTKEVVHMDCLMLAHNPGGKERTEEEFEALAKGSGFQGFQVVCKAYGTHIIELIKKM
>Mpyg13223.t1
MGSTAETQITPVQVTDDEAALFAMQLASASVLPMALKSALELDLLEIMAKNSSPMSPSEIASHLPTKNPEAPVMLDRILRLLTAYSVLTCSVRTLPGGDGVERIYGLGPVCKYLTKNEDGVSLAALCLMNQDKVLMESWYHLKDAILDGGIPFNKAYGMSAFEYHGTDPRFNKVFNNGMSNHSTITMKKILETYKGFESLTSLVDVGGGIGATLKMIVSKYPNLKGINFDLPHVIDDAPSYPGIEHVGGDMFVSVPKGDAIFMKWICHDWSDEHCVKFLKNCYEALPEDGKVILAECILPETPDSSLSTKQVVHVDCIMLAHNPGGKERTEKEFEALAKGSGFKGIKVVCDAFGVHLIELLKKL
>Mpyg18483.t1
MRLSTDEKCKQIMKNRYNALPVGGKLIACEPVLPKETDDSHRTRALLEGDIFVMTIYRTKGKHRTEEEFKEFGLSAGFPTFRPFYLDYFYTILGFQK
>Mpyg19722.t1
MSNHLQESLTTYPNPVLTKEEQEVDEKMVSLQAESIVNTVAFPMVFKAALELGVIDTLAAAGNGTWLSPSEIAVSLPTKPTNPEASVLLDRMLRLLVSHSILKCRMVEIKENSRTGNIERVYAAEPVCKFFLKDSDGSGSLASLFMLLQSEVLFKTWTNLKDVILEGRDGFSSAHGMRLFEYIGSDEQFAEVFNRAMSEPSTLIMKKVLEVYRGFEDVNTLVDVGGGTGTILGLVTSKYPHIKGVNFDLVQVISRAPFYPGVEHISGDMFIEIPKGDAIFMKWILHDWTDERCIKILKNCWKSLPEKGKVIIVDMITPMEPKTGDSSSIVFGMDLLMLTQCSGGKERSLSQLENLAFDSGFLRCEIICSVYSYSVIEFHK
>Mpyg21383.t1
MSLFMLLQSEVHFKTWSHLKDVILEGKDAFSSAYGMRVFEYIGSNERYGEMFNRAMSEASTLIMTKVLEVYKGFCKTR
>Mpyg19721.t1
MSNHLQEPTYPKLVLTKEEQKADDEMVSLEAESIANTVAFPMVLNAALEHGVIDTVAAARKDWKPMLGLLGFRAGPAWLSPSEIAVSLPTKPINPEAPVLLDRMLRLLVSHSFLKCRMTETGESGRSEKIKRVYAAEPMWEEVAQVLSHAPFYPGVEHVFIEVPKGDAIFMKILHDWTDGRTLCKDFEKLLEESSRKRKSDNCRHDYANGTKDWRLFLYCVRHGLVDVNTMLGW
>Mpyg19785.t1
MGHPFRETLSLSSKINDDDTELGLVAVRLANCITFPMVFKAAIELGVIDTLYLAARADVNGSGSFLTPSEVATRLPTKPSNPEAPVLLDRMLRLLASYSMVKCQVVESERVYKAEPICRFFLKDSVDELGTLASQFILGMDSVFLNTWAQLKDVVLEGGDAFGRANGGLKLFDYMGTDERLNKLFNRTGFSGGVLKKFVEVYRGFEGVNVLVDVGGGVGNTLSFITSKYPNIKGINFDLTCALTQALSNPNVEHVAGDMFVDVPRGDAIILKRILHDWNDEDCEKILKNCCKASPENGKVIVMELVIPDEAEIGDVQSNIAFDMDLLMLTQCSGGKERSRAEYKALAAKSGFSNCNFVCRAYHLWVIELSK
>Mpyg21384.t1
MAFVDEGVWFSSSEIALRLPTKPTNPEAPVLLDRMLVLLASHSILKYRLAETERLYAAEPVCMFFLNRGDGSGSLPALFMVALSDVYFKTWAHLKDVLIEGKDAFSSAHGMRLFEYIGSNEQFAEMFNRAMSETSTMTMNKVLEVYRGLIEDVNTLVDVGGGIGAIIGLVISKYPHIKGIDFDQPSVLTRAPLYPGVEHVSGDMFTEIPKGDAIFMKLGC
MGYLFEETLSSNPKTPVVTDDDNELGLMAVRLANAAAFPMVLKAALELGVFDTLHAASEFLSPSEIASRLPTTPRNPEAPVLLDRMLRLLASYSMVKCGKVSEGKGERVYKAEPICRFFLKNNIQDIGSLASQVIVNFDSVFLNTWAQLKDVVLEGGDAFGRAHGGMKLFDYMGTDERFSKLFNQTGFTIAVVKKALQVYEGFKGVNVLVDVGGGVGNTLGVVTSKYPNIKGINFDLTCALAQAPSIHGVEHVAGDMFVDVPTGDAMILKRILHDWTDEDCVKILKNCWKSLPENGKVIVIELVTPDEAENGDINANIAFDMDMLMFTQCSGGKERSRAEFEALAAASGFTRCKFVCPAYHCWIIEFIK
>Mpyg01407.t1
MEEQNLSLYIMILSSSSVLPMVLKTAIDLGLFDILAESAPSPLSASQIVSLLYTQTQQDHDSSLVNRILRCLASYSIVTCSVSTDHGEPRAVYGLAPVAKYFTKNQDGCGSLAPLVNLFQDKVVTDIWYNLKDSVLEGGLPFHKTHGSSAVELVGRDSRFREVFQSSMRGFNEVFMKEFLKNYNGFDGVKSLVDVGGGDGSILSEIISKHPHIIKAINFDLPSVINTSSASPGIGHVSGDMFTNIPKGEAIFMKWMLHSWDDENCVKILRNCYQSLPSNGKVIVVDMVVPDLPRDSLLDRSLFQFELFMMNMNPSGKERTKREFEILARLAGFSSVQVPFTALCFSVVEFHKNV
>Mpyg02344.t1
MGYLSEETLSSNLKTPVVTDDDNELGLMAVRLANAAAFPMVLKAALELGVFDTLHAASEFLSPSEIASRLPTTPRNPEAPILLDRMLRLLASYSMVKCGKVSEGKGERVYKAEPICRFFLKNNIQDIGSLASQVIVNFDSVFLNTWAQLKDVVLEGGDAFGRAHGGMKLFDYMGTDERFSKLFNQTGFTIAVVKKALEVYEGFKGVNVLVDVGGGVGNTLAVVTSKYPNIKGINFDLTCALAQAPSIPGVEHVAGDMFVDVPTGDAMILKRILHDWTDEDCVKILKNCWKSLPENGKVVVIELVTPDDAENGDINANIAFDMDMLMFTQCSGGKERSRAEFEALAAESGFTHCKFVCPAYHCWIIEFIK
>Mpyg09481.t1
MYSTAETQITPVQVTDDETSLFAMQLASCSVLPMVLQTALELDLLEIMAKNASQMSPAEITSHLPTKNPEAPVILDRILRLLAAYSILTCSVRTLPGGVGLDRLYGLGPVCKYLTKNEDGVSIAALCLMTQDKVFMESWYHLKDAILDGGSPFNKAYGMSVLEYQKTDARFNKVVTDGMSNHSTISMKKILETYKGFDGLTSLVDVGGGNGATLKMIVSKYPNLKGVNFDLPHVIKNAPSYSGIEHVGGDMFVSVPKGDAIFMKWICHARSDEQCFKFLKNCYDALPENGKVIVAECILPETLDSSVLTKQALHVDCIMLAHSRGGIERTEKEYEALAKGSGFHGIKVVCNAFGIYIIEFLKKN
>Mpyg08257.t1
MGSLAETQITPAKISDEEANLFAMQLAGATVLPMVLTSALELDLLEIISKNAGLAGGQLSPSEIASHLPTKNPDAPIMVDRILRLLAAYSILTCSVRKLLDGGVERLYGLGPVCKYLTKNEDGVSLAALCHLNRDRVFMESWYHLKDAVLEGGVPFDKAFGMDAFEYQGTDPRFNKVFNNGMSNHTTIVMTKILETYKGFEELSSLVDVGGGIGVTLRMIVSKHPHIKGILYDLSHVIEEAVSYPGIEHIGGDMFVNVPKADAIFMKWILHDWSDQHCLKVLKNCYEALPDNGKVIVAESILPVVPDSSLLTKEVVHMDCLMLAHNPGGKERTEEEFEALAKGSGFQGFQVVCKAYGTHIIELIKKM
>Mpyg13223.t1
MGSTAETQITPVQVTDDEAALFAMQLASASVLPMALKSALELDLLEIMAKNSSPMSPSEIASHLPTKNPEAPVMLDRILRLLTAYSVLTCSVRTLPGGDGVERIYGLGPVCKYLTKNEDGVSLAALCLMNQDKVLMESWYHLKDAILDGGIPFNKAYGMSAFEYHGTDPRFNKVFNNGMSNHSTITMKKILETYKGFESLTSLVDVGGGIGATLKMIVSKYPNLKGINFDLPHVIDDAPSYPGIEHVGGDMFVSVPKGDAIFMKWICHDWSDEHCVKFLKNCYEALPEDGKVILAECILPETPDSSLSTKQVVHVDCIMLAHNPGGKERTEKEFEALAKGSGFKGIKVVCDAFGVHLIELLKKL
>Mpyg18483.t1
MRLSTDEKCKQIMKNRYNALPVGGKLIACEPVLPKETDDSHRTRALLEGDIFVMTIYRTKGKHRTEEEFKEFGLSAGFPTFRPFYLDYFYTILGFQK
>Mpyg19722.t1
MSNHLQESLTTYPNPVLTKEEQEVDEKMVSLQAESIVNTVAFPMVFKAALELGVIDTLAAAGNGTWLSPSEIAVSLPTKPTNPEASVLLDRMLRLLVSHSILKCRMVEIKENSRTGNIERVYAAEPVCKFFLKDSDGSGSLASLFMLLQSEVLFKTWTNLKDVILEGRDGFSSAHGMRLFEYIGSDEQFAEVFNRAMSEPSTLIMKKVLEVYRGFEDVNTLVDVGGGTGTILGLVTSKYPHIKGVNFDLVQVISRAPFYPGVEHISGDMFIEIPKGDAIFMKWILHDWTDERCIKILKNCWKSLPEKGKVIIVDMITPMEPKTGDSSSIVFGMDLLMLTQCSGGKERSLSQLENLAFDSGFLRCEIICSVYSYSVIEFHK
>Mpyg21383.t1
MSLFMLLQSEVHFKTWSHLKDVILEGKDAFSSAYGMRVFEYIGSNERYGEMFNRAMSEASTLIMTKVLEVYKGFCKTR
>Mpyg19721.t1
MSNHLQEPTYPKLVLTKEEQKADDEMVSLEAESIANTVAFPMVLNAALEHGVIDTVAAARKDWKPMLGLLGFRAGPAWLSPSEIAVSLPTKPINPEAPVLLDRMLRLLVSHSFLKCRMTETGESGRSEKIKRVYAAEPMWEEVAQVLSHAPFYPGVEHVFIEVPKGDAIFMKILHDWTDGRTLCKDFEKLLEESSRKRKSDNCRHDYANGTKDWRLFLYCVRHGLVDVNTMLGW
>Mpyg19785.t1
MGHPFRETLSLSSKINDDDTELGLVAVRLANCITFPMVFKAAIELGVIDTLYLAARADVNGSGSFLTPSEVATRLPTKPSNPEAPVLLDRMLRLLASYSMVKCQVVESERVYKAEPICRFFLKDSVDELGTLASQFILGMDSVFLNTWAQLKDVVLEGGDAFGRANGGLKLFDYMGTDERLNKLFNRTGFSGGVLKKFVEVYRGFEGVNVLVDVGGGVGNTLSFITSKYPNIKGINFDLTCALTQALSNPNVEHVAGDMFVDVPRGDAIILKRILHDWNDEDCEKILKNCCKASPENGKVIVMELVIPDEAEIGDVQSNIAFDMDLLMLTQCSGGKERSRAEYKALAAKSGFSNCNFVCRAYHLWVIELSK
>Mpyg21384.t1
MAFVDEGVWFSSSEIALRLPTKPTNPEAPVLLDRMLVLLASHSILKYRLAETERLYAAEPVCMFFLNRGDGSGSLPALFMVALSDVYFKTWAHLKDVLIEGKDAFSSAHGMRLFEYIGSNEQFAEMFNRAMSETSTMTMNKVLEVYRGLIEDVNTLVDVGGGIGAIIGLVISKYPHIKGIDFDQPSVLTRAPLYPGVEHVSGDMFTEIPKGDAIFMKLGC
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University