Enzyme
This enzyme is a member of the aromatic amino acid lyase family, other members of which are EC 4.3.1.3 (histidine ammonia-lyase) and EC 4.3.1.23 (tyrosine ammonia-lyase) and EC 4.3.1.25 (phenylalanine/tyrosine ammonia-lyase). The enzyme contains the cofactor 3,5-dihydro-5-methylidene-4H-imidazol-4-one (MIO), which is common to this family [3]. This unique cofactor is formed autocatalytically by cyclization and dehydration of the three amino-acid residues alanine, serine and glycine [9]. The enzyme from some species is highly specific for phenylalanine [7,8].
PAL
Enzyme
Phenylalanine ammonia-lyase
Enzyme name
EC:4.3.1.24
Entry
Lyases;Carbon-nitrogen lyases;Ammonia-lyases
Class
L-phenylalanine ammonia-lyase (trans-cinnamate-forming)
Sysname
L-phenylalanine = trans-cinnamate + NH3[RN:R00697]
Reaction
trans-cinnamate [CPD:C00423];NH3 [CPD:C00014]
Product
Species information
Megacarpaea delavayi
Species name
Megacarpaea delavayi is a species of eudicot in the family Brassicaceae (mustard family).
Species info
Gene sequence info
Megacarpaea_delavayi.PAL.fa
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>Mde001672.5.t1
MLGFRRNIWEHEGEMSHAAAIRHKSRHARQVNTILQGYSGIRFDILEAITSLLNHNITPSLLLRGTITASGDLIPLSFIVGLLTGRPNSKAICRNGESLTAEEAFEKAKISSGFFDLQPKEGLALVNGTAVGSGMASMVLFEANVQSVLAEVLSAIFAEVMSEKPEFTDHLPTLTLSFLLFYLNIVLN
>Mde009226.1.t1
MAGISSGFFDMQHKEGLALVNGTAVGSGMASMVLFETNVLSVLAEIPSAVFAEVMSGKPEFTDHLTHRLKHHPGQIEAAAIMEFF
>Mde013792.1.t1
MTSSNLTIIFEYWMDKGNVERCVDDEAEGESKATKCREVRIKFKACIYDNPVYYEPIISAQAQVVAKMLSELQAEKEAVLAAEAAVIAMALSSLQIEEQPVMSAEVDINRRKRDFAGIFGNAKETCHTLPQSATRAAMLVRVNTLLQGYSGIRFDIVEAITSLLNHNITPSLLLHGTITASGDLVPLSYIDGLLTGRPNSKAIFGPNGESLTAEEAFEKAGINSGFFDLQPKEGLALVNGTEVGSGMASMVLFEANVQSVLAEVLSAIFAEVMSGKPEFTDHLPTLTLRFISADTLLV
>Mde015131.6.t1
MELCKQSNNHITAVSGDPLNWNAAAESLKGSHLEEVKRMVEDYRKGTVLLGGETLTIGQVAAVARGGGGATVELAEEARAGVKASSEWVMESMNRGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGIFGAGIGDTSHTLPKPPTRAAMLVRVNTLLQGYSGIRFEILEAITKLLNHEITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAVGPSGEILTASDAFKLAGVTSFFELQPKEGLALVNGTAVGSGLASTVLFEANVLAVLSEVMSAMFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAIMEHILDGSSYVKEAQHLHEMDPLQKPKQDRYALRTSPQWLGPQIEVIRAATKMIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNSRLAIASIGKLMFAQFSELVNDFYNNGLPSNLSGGRNPSLDYGFKGAEIAMASYCSELQFLANPVTNHVQSAEQHNQDVNSLGLISSRKTAEAVDILKLMSTTYLVALCQAVDLRHLEENLKKAVKAAVSQVAKRVLTVGANGELHPSRFTERDVLQVVDREYVFSYADDPCSLTYPLMQKLRHILVDHALKDPDREANLETSVFQKIGLFESELKLLLPKEVERVRMEYEEGSSVIDNRIKECRSYPLYRFVRNELETELLTGENVRSPGEEFDKVFSAICDGKLIDPLLECLKVWNGAPIPIC
>Mde752126.1.t1
HRRTKNGVALQTELIRFLNAGIFGNTKETCHTLPQSATRAAMLVRVNTLLQGYSGIRFDILEAITSLLNHNITPSLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGESLTAEEAFEKAGISSGFFDLQPKEGLALVNGTAVGSGMASMVLFEANVQSVLAEVLSAIFAEVMSGKPEFTDHLTHRLKHHPGQIEAAAIMEHILDGSSYMKLAQKLHEMDPLQKPKQDRYALRTSPQWLGPQIEVIRQATKSIEREINSVNDNPLIDVSRNKAIHGGNFQGTPIGVSMDNTRLAIAAIGKLMFAQFSELVNDFYNNGLPSNLTASNNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTSEAVDILKLMSTTFLVAICQAVDLRHLEENLRQTVKNTISQVAKKVLTTGINGELHPSRFCEKDLLKVVDREQVFTYIDDPCSATYPLMQKLRQVIVDHALSNGETEKNAATSIFQKIGAFEEELKAVLPKEVDATREAYGNGAAAIPNRIKDCRSYPLYRFVREELGTKLLTGEKVVSPGEEFDKVFTAMCEGKLIDPMMDCLKEWNGAPIPIC
MLGFRRNIWEHEGEMSHAAAIRHKSRHARQVNTILQGYSGIRFDILEAITSLLNHNITPSLLLRGTITASGDLIPLSFIVGLLTGRPNSKAICRNGESLTAEEAFEKAKISSGFFDLQPKEGLALVNGTAVGSGMASMVLFEANVQSVLAEVLSAIFAEVMSEKPEFTDHLPTLTLSFLLFYLNIVLN
>Mde009226.1.t1
MAGISSGFFDMQHKEGLALVNGTAVGSGMASMVLFETNVLSVLAEIPSAVFAEVMSGKPEFTDHLTHRLKHHPGQIEAAAIMEFF
>Mde013792.1.t1
MTSSNLTIIFEYWMDKGNVERCVDDEAEGESKATKCREVRIKFKACIYDNPVYYEPIISAQAQVVAKMLSELQAEKEAVLAAEAAVIAMALSSLQIEEQPVMSAEVDINRRKRDFAGIFGNAKETCHTLPQSATRAAMLVRVNTLLQGYSGIRFDIVEAITSLLNHNITPSLLLHGTITASGDLVPLSYIDGLLTGRPNSKAIFGPNGESLTAEEAFEKAGINSGFFDLQPKEGLALVNGTEVGSGMASMVLFEANVQSVLAEVLSAIFAEVMSGKPEFTDHLPTLTLRFISADTLLV
>Mde015131.6.t1
MELCKQSNNHITAVSGDPLNWNAAAESLKGSHLEEVKRMVEDYRKGTVLLGGETLTIGQVAAVARGGGGATVELAEEARAGVKASSEWVMESMNRGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGIFGAGIGDTSHTLPKPPTRAAMLVRVNTLLQGYSGIRFEILEAITKLLNHEITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAVGPSGEILTASDAFKLAGVTSFFELQPKEGLALVNGTAVGSGLASTVLFEANVLAVLSEVMSAMFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAIMEHILDGSSYVKEAQHLHEMDPLQKPKQDRYALRTSPQWLGPQIEVIRAATKMIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNSRLAIASIGKLMFAQFSELVNDFYNNGLPSNLSGGRNPSLDYGFKGAEIAMASYCSELQFLANPVTNHVQSAEQHNQDVNSLGLISSRKTAEAVDILKLMSTTYLVALCQAVDLRHLEENLKKAVKAAVSQVAKRVLTVGANGELHPSRFTERDVLQVVDREYVFSYADDPCSLTYPLMQKLRHILVDHALKDPDREANLETSVFQKIGLFESELKLLLPKEVERVRMEYEEGSSVIDNRIKECRSYPLYRFVRNELETELLTGENVRSPGEEFDKVFSAICDGKLIDPLLECLKVWNGAPIPIC
>Mde752126.1.t1
HRRTKNGVALQTELIRFLNAGIFGNTKETCHTLPQSATRAAMLVRVNTLLQGYSGIRFDILEAITSLLNHNITPSLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGESLTAEEAFEKAGISSGFFDLQPKEGLALVNGTAVGSGMASMVLFEANVQSVLAEVLSAIFAEVMSGKPEFTDHLTHRLKHHPGQIEAAAIMEHILDGSSYMKLAQKLHEMDPLQKPKQDRYALRTSPQWLGPQIEVIRQATKSIEREINSVNDNPLIDVSRNKAIHGGNFQGTPIGVSMDNTRLAIAAIGKLMFAQFSELVNDFYNNGLPSNLTASNNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTSEAVDILKLMSTTFLVAICQAVDLRHLEENLRQTVKNTISQVAKKVLTTGINGELHPSRFCEKDLLKVVDREQVFTYIDDPCSATYPLMQKLRQVIVDHALSNGETEKNAATSIFQKIGAFEEELKAVLPKEVDATREAYGNGAAAIPNRIKDCRSYPLYRFVREELGTKLLTGEKVVSPGEEFDKVFTAMCEGKLIDPMMDCLKEWNGAPIPIC
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University