Enzyme
Specific for quercetin. Related enzymes bring about the 3-O-methylation of other flavonols, such as galangin and kaempferol.
COMT
Enzyme
Flavone 3'-O-methyltransferase
Enzyme name
EC:2.1.1.76
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:3,5,7,3',4'-pentahydroxyflavone 3-O-methyltransferase
Sysname
S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone = S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone [RN:R02157]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];3-methoxy-5,7,3',4'-tetrahydroxyflavone [CPD:C04443]
Product
Species information
Manihot esculenta
Species name
Cassava (Manihot esculenta) is a species of eudicot in the family Euphorbiaceae (spurge family).
Species info
Gene sequence info
Manihot_esculenta.COMT.fa
Download
>rna-XM_021771577.1
MGSTAETQMTPTQISDEEANLFAVQLTSASVLPMVLKSAIELDLLEIIGKAGPGAFLSPTDIASQLPTSNPDAPVMLDRILRLLASYSILKFSPRTLPDGKVERLYGLAPVCKFLTKNEDGVSIAALCLMNQDKVLIESWYHLKDAVLEGGIPFNKAYGMTAFEYHGTDPRFNKVFNKGMADHSTLAMKKILETYEGFQGLSSIVDVGGGTGAVLSMIVSKYPSIKGINFDLPHVIDDAPPHPGVEHVGGDMFVSVPKGDAIFMKWICHDWSDEHCLKFLKNCYNALPPHGKVIVAECILPVAPDNSLATKTVVHIDCIMLAHNPGGKERTEKEFEALAKGAGFQGFRVVCRAFHTYVMEFLKSA
>rna-XM_021771599.1
MGSTAETQMTPTQISDEEANLFAMQLTSASVLPMVLKSAIELDLLEIIGKAGPGAFLSPTDIASQLPTSNPDAPVMLDRILRLLASYSILKFSLRTLPDGKVERLYGLAPVCKFLTKNEDGVSIAALCLMNQDKVLMESWYHLKDAVLEGGIPFNKAYGMTAFEYHGTDPRFNKVFNKGMADHSTITMKKILETYEGFQGLSSIVDVGGGTGAVLSMIVSKYPSIKGINFDLPHVIDDAPPHPGVEHVGGDMFVSVPKGDAIFMKWICHDWSDEHCLKFLKNCYNALPPHGKVIVAECILPVAPDNSLATKTVVHIDCIMLAHNPGGKERTEKEFEALAKGAGFQGFRVVCRAFHTYVMEFLKSA
>rna-XM_021749650.1
MASITTTHEQQEEEEQRQYAMQIASASLLPMALKAATELGVLEILEKAGPTVQLSSSQIASQLTILNPLLLDCILRLLASNRVLTCSVTSNGHDGQLHSLYGLGPVAKYFIKNHDGGCLAPLLAMIQDKVMMETWYHLKDAVVEGGTPFDKAHKMNGIEYMGMEQWILHMWDDQDVIKLLKNCYEALPSNGKVIAVDMVVPETTGTNTAARSLLQTCLLLTSMNSKGKERTYREIQTLAKKAGFSEVQVACCAYNFSVVEFLKHV
>rna-XM_021765882.1
MDEEEDGLFAMQLATVSVVPMVLRTAMELNLLEMIAEKGEGGQSSAFELASRLSTKNPDAPNIVDRMLRLLASYSILTCSTVTDNQGHAQNLYGLAPICKYYTRDKDGVSLVPYVTTFLTRPFIDSWYHLKEAVLEGAAPFEKANGMEIFEFVRRNKTGFNETMFNNTMMVMRKFLEKYKGFEDLHQLVDVGGGFGANLSLIVSKYPQIQGINFDLPHVVQDALPCAGVKHVGGDMFVNIPKGEAIFMKWILHDWDDDQCVKILKNCYDALPEFEKVIAVESVIPKFVETDVRSKNVFKLDMCMFISDNLRSNRIGFYKGLSIEE
>rna-XM_021763472.1
MEDESSETRKRARVAILELANMISVPMSLNAVVRLNVADAIWQGGTNSPLSASEILARIHPSGGDPENLQRILRMLTSYGVFAEHLNADAIERKYSLTEIGKTLVTDGEGLSYAPYVLQHHQDALMRAWPLVHEAVLDSTTEPFVKANDEPAYDYYGKRPEMNGLMLKAMSGVSVPFMKAVLKGYSGFQGLERLVDVGGSAGDCLRMILHKYPIVQGINFDLPEVVAKAPNIPGVTHVGGDMFKSIPSADGIFMKWVLTTWTDYECKQIMENCYNALPVGGKLIACEPVLPKDSDDSHRTRALLEGDIFVMTIYRAKGKHRTEEEFKQLGHSVGFSHFQAFYVDYFYSILEFQK
>rna-XM_021764362.1
MNILEKLEKSSIDGEEDGLFAMQLAKISILPMVLKTTMELDLLEIMAEKGEGAQSSASELASRLPTKNPDAPSIVDKMLRLLASYSILTCSTVTDEQGHVQNLYGLAPVCKFLTRDRDESSLAPYTLMSLSRPFIAAWDHLKEAVLEGVSPFEKENGMGLFEFVGRNKTLFNEAMYHNTMMVMTKLLEKYKGFEGLHQLLDVGGGLGANLRLIVSKYPQIQGINFDLPHVVQDAPPCPGVEHVGGDMFVNIPKGEVIFLKWILHDWDDDRCTKILKNCYDALPETGKVIVVEAVIPEFVETDVRSRNVFKLDMTMINATSGGKERTEKELESLARRAGFAAIKLICSAYCFSIFEFYKRP
>rna-XM_021765880.1
MMNTLLGKLEKLSIDGEEDGLFAMQLATISILPMVLRTTLELDLLEIMAEKGEGGQSSASELASRLPTKNPDAPSILERMLRLLASYSILTCSTVTDEQGNAQNLYGLTPVCKFFTKNIDGVSLAPYAISLMSRPAIGSWFHLKEAVLEGVSPFEKANGMGIFEFVRRNKTVFNESMYSHTMIVMKKFLEKYKGFEGLHQLVDVGGGLGANLSLIVSKYPQIKAINFDLPHVIHDAPPCPGVEHVGGDMFVNIPKGEAIFMKWILHDWDDDQCLKILKNCYDALPEFGKVIVVESVIPEFVETDVLSRNVFKLDMNMLIAIPGGKERTEKEFETLAKVAGFAAVKLIDRAYSYSILEFYKRP
>rna-XM_021764155.1
MMNTQLGKLEKLSIDGEEDALFAMQLATISILPVVLRTTLELDLLEIMAEKGEGGQSSASELASRLPTKNPDAPSIVERMLRLLASYSILTCSTVTDEQGNAQNLYGLAPVCKFFTKDIDGVSLAPYAISLMSRPAIGSWFHLKEAVLEGVSPFEKANGMGIFEFVRRNKTIFNESMYNHTMIVMKKFLEKYKGFKGLHQLVDVGGGLGANLSLIVSKYPQIKAINFDLPHVVHDAPPCPGVKHVGGDMFANIPKGEAIFMKWILHDWDDDQCLKILKNCYDALPEFGKVIVVESVIPEFVETDVLSRNVFKLDINMLIAIPGGKERTEKEFETLAKVAGFAAVKLIDRAYSYSILEFYKRP
>rna-XM_021773099.1
MAMTHGIDLSCEDSVENLLKLFDIIAQAGPLTAAEITAHLPTRNPDAPSMIDRLLRLLASYSLLTSSHGLIERRYGLSSTGQFFVRDNKHGVSMAAFPADAKALTEAWFYLKDSILEGGNPFERAHGVPMYKYISSDQESVKDFSKAMDSISSYIISKVLDSYNGFQGLKSLVDVGGGSGVAINMVISKYPSIAGINFDLPHVVKEAPPHLGVKHVGGDMLSGIPSADAIMMKDVLHNWSDEQCTKILKNCYDALPEGGKVIVVSHIMPRALDSSIATKYVCQLDVMMFLFPGGKQRTEEEFKDLAKATGFSRFQLICYVAYDAVGVMEFYK
>rna-XM_021774324.1
MNSKLIQMATSTIINEEEEEYYCQYAMQLASASVLPMALKAATDLGVLEIIEKAGPSAQLSSSQIASQLASPAAQTAPHVLDRILLVLASNGILTCSATATIGTEDGQLSRFFGLAPVAKYFIKNHDRGCLAPFLAVIHDKVIMDTWHHLKDAVVEGGNTFEKAHGMSFMEFKGKDSSFVEMFKDSMRDFNTLLMRRILDKYDGFQGLMSLVDVGGGDGSILNMIISKYPSIKGINYDLAPVIEKSPSYAGIQHVAGDMFMNVPKGEAIFVKWVLHMWDDEHCLKLLKNCYEALPSDGKLIAVDMVVPEAIGDNVAAKGLLQTHLLFTNMNPKRNDRTEREFQTLGKKAGFSHVGVAACAFNFSVVEFLKLI
>rna-XM_021745087.1
MTGSEQSASATIVSECCDEEETWNLAVSLANAVVLPMVLKSALELNIIDIISTPGNSRGSLSASEIAQRIPAARNQDAPILLDRMLRVLASYDIVKCSSSTKENGQVERWYGAGPICKFLTKNQDGSGSAGPLLLMLHDEVLMKSWFHLNDAILEGGFPFSRAHGMTAFEYLGTDQRFNTLFNQAMSSYTTLVVKKILDVYRGFDGLNVLVDVGGGTGVTLSIIASKYPHIKGINYDLPHVLANSTSYSGVEHVKGDMFTSVPKGDAIFLKWILHDWSDEHCLKLLKNCWEALPSNGKVIVVECILPMAPENIVSSHFVHKQDLLMLTQSPGGKERTPKEFEALALKSGFSSCEAICCAYNSWVLEFHK
MGSTAETQMTPTQISDEEANLFAVQLTSASVLPMVLKSAIELDLLEIIGKAGPGAFLSPTDIASQLPTSNPDAPVMLDRILRLLASYSILKFSPRTLPDGKVERLYGLAPVCKFLTKNEDGVSIAALCLMNQDKVLIESWYHLKDAVLEGGIPFNKAYGMTAFEYHGTDPRFNKVFNKGMADHSTLAMKKILETYEGFQGLSSIVDVGGGTGAVLSMIVSKYPSIKGINFDLPHVIDDAPPHPGVEHVGGDMFVSVPKGDAIFMKWICHDWSDEHCLKFLKNCYNALPPHGKVIVAECILPVAPDNSLATKTVVHIDCIMLAHNPGGKERTEKEFEALAKGAGFQGFRVVCRAFHTYVMEFLKSA
>rna-XM_021771599.1
MGSTAETQMTPTQISDEEANLFAMQLTSASVLPMVLKSAIELDLLEIIGKAGPGAFLSPTDIASQLPTSNPDAPVMLDRILRLLASYSILKFSLRTLPDGKVERLYGLAPVCKFLTKNEDGVSIAALCLMNQDKVLMESWYHLKDAVLEGGIPFNKAYGMTAFEYHGTDPRFNKVFNKGMADHSTITMKKILETYEGFQGLSSIVDVGGGTGAVLSMIVSKYPSIKGINFDLPHVIDDAPPHPGVEHVGGDMFVSVPKGDAIFMKWICHDWSDEHCLKFLKNCYNALPPHGKVIVAECILPVAPDNSLATKTVVHIDCIMLAHNPGGKERTEKEFEALAKGAGFQGFRVVCRAFHTYVMEFLKSA
>rna-XM_021749650.1
MASITTTHEQQEEEEQRQYAMQIASASLLPMALKAATELGVLEILEKAGPTVQLSSSQIASQLTILNPLLLDCILRLLASNRVLTCSVTSNGHDGQLHSLYGLGPVAKYFIKNHDGGCLAPLLAMIQDKVMMETWYHLKDAVVEGGTPFDKAHKMNGIEYMGMEQWILHMWDDQDVIKLLKNCYEALPSNGKVIAVDMVVPETTGTNTAARSLLQTCLLLTSMNSKGKERTYREIQTLAKKAGFSEVQVACCAYNFSVVEFLKHV
>rna-XM_021765882.1
MDEEEDGLFAMQLATVSVVPMVLRTAMELNLLEMIAEKGEGGQSSAFELASRLSTKNPDAPNIVDRMLRLLASYSILTCSTVTDNQGHAQNLYGLAPICKYYTRDKDGVSLVPYVTTFLTRPFIDSWYHLKEAVLEGAAPFEKANGMEIFEFVRRNKTGFNETMFNNTMMVMRKFLEKYKGFEDLHQLVDVGGGFGANLSLIVSKYPQIQGINFDLPHVVQDALPCAGVKHVGGDMFVNIPKGEAIFMKWILHDWDDDQCVKILKNCYDALPEFEKVIAVESVIPKFVETDVRSKNVFKLDMCMFISDNLRSNRIGFYKGLSIEE
>rna-XM_021763472.1
MEDESSETRKRARVAILELANMISVPMSLNAVVRLNVADAIWQGGTNSPLSASEILARIHPSGGDPENLQRILRMLTSYGVFAEHLNADAIERKYSLTEIGKTLVTDGEGLSYAPYVLQHHQDALMRAWPLVHEAVLDSTTEPFVKANDEPAYDYYGKRPEMNGLMLKAMSGVSVPFMKAVLKGYSGFQGLERLVDVGGSAGDCLRMILHKYPIVQGINFDLPEVVAKAPNIPGVTHVGGDMFKSIPSADGIFMKWVLTTWTDYECKQIMENCYNALPVGGKLIACEPVLPKDSDDSHRTRALLEGDIFVMTIYRAKGKHRTEEEFKQLGHSVGFSHFQAFYVDYFYSILEFQK
>rna-XM_021764362.1
MNILEKLEKSSIDGEEDGLFAMQLAKISILPMVLKTTMELDLLEIMAEKGEGAQSSASELASRLPTKNPDAPSIVDKMLRLLASYSILTCSTVTDEQGHVQNLYGLAPVCKFLTRDRDESSLAPYTLMSLSRPFIAAWDHLKEAVLEGVSPFEKENGMGLFEFVGRNKTLFNEAMYHNTMMVMTKLLEKYKGFEGLHQLLDVGGGLGANLRLIVSKYPQIQGINFDLPHVVQDAPPCPGVEHVGGDMFVNIPKGEVIFLKWILHDWDDDRCTKILKNCYDALPETGKVIVVEAVIPEFVETDVRSRNVFKLDMTMINATSGGKERTEKELESLARRAGFAAIKLICSAYCFSIFEFYKRP
>rna-XM_021765880.1
MMNTLLGKLEKLSIDGEEDGLFAMQLATISILPMVLRTTLELDLLEIMAEKGEGGQSSASELASRLPTKNPDAPSILERMLRLLASYSILTCSTVTDEQGNAQNLYGLTPVCKFFTKNIDGVSLAPYAISLMSRPAIGSWFHLKEAVLEGVSPFEKANGMGIFEFVRRNKTVFNESMYSHTMIVMKKFLEKYKGFEGLHQLVDVGGGLGANLSLIVSKYPQIKAINFDLPHVIHDAPPCPGVEHVGGDMFVNIPKGEAIFMKWILHDWDDDQCLKILKNCYDALPEFGKVIVVESVIPEFVETDVLSRNVFKLDMNMLIAIPGGKERTEKEFETLAKVAGFAAVKLIDRAYSYSILEFYKRP
>rna-XM_021764155.1
MMNTQLGKLEKLSIDGEEDALFAMQLATISILPVVLRTTLELDLLEIMAEKGEGGQSSASELASRLPTKNPDAPSIVERMLRLLASYSILTCSTVTDEQGNAQNLYGLAPVCKFFTKDIDGVSLAPYAISLMSRPAIGSWFHLKEAVLEGVSPFEKANGMGIFEFVRRNKTIFNESMYNHTMIVMKKFLEKYKGFKGLHQLVDVGGGLGANLSLIVSKYPQIKAINFDLPHVVHDAPPCPGVKHVGGDMFANIPKGEAIFMKWILHDWDDDQCLKILKNCYDALPEFGKVIVVESVIPEFVETDVLSRNVFKLDINMLIAIPGGKERTEKEFETLAKVAGFAAVKLIDRAYSYSILEFYKRP
>rna-XM_021773099.1
MAMTHGIDLSCEDSVENLLKLFDIIAQAGPLTAAEITAHLPTRNPDAPSMIDRLLRLLASYSLLTSSHGLIERRYGLSSTGQFFVRDNKHGVSMAAFPADAKALTEAWFYLKDSILEGGNPFERAHGVPMYKYISSDQESVKDFSKAMDSISSYIISKVLDSYNGFQGLKSLVDVGGGSGVAINMVISKYPSIAGINFDLPHVVKEAPPHLGVKHVGGDMLSGIPSADAIMMKDVLHNWSDEQCTKILKNCYDALPEGGKVIVVSHIMPRALDSSIATKYVCQLDVMMFLFPGGKQRTEEEFKDLAKATGFSRFQLICYVAYDAVGVMEFYK
>rna-XM_021774324.1
MNSKLIQMATSTIINEEEEEYYCQYAMQLASASVLPMALKAATDLGVLEIIEKAGPSAQLSSSQIASQLASPAAQTAPHVLDRILLVLASNGILTCSATATIGTEDGQLSRFFGLAPVAKYFIKNHDRGCLAPFLAVIHDKVIMDTWHHLKDAVVEGGNTFEKAHGMSFMEFKGKDSSFVEMFKDSMRDFNTLLMRRILDKYDGFQGLMSLVDVGGGDGSILNMIISKYPSIKGINYDLAPVIEKSPSYAGIQHVAGDMFMNVPKGEAIFVKWVLHMWDDEHCLKLLKNCYEALPSDGKLIAVDMVVPEAIGDNVAAKGLLQTHLLFTNMNPKRNDRTEREFQTLGKKAGFSHVGVAACAFNFSVVEFLKLI
>rna-XM_021745087.1
MTGSEQSASATIVSECCDEEETWNLAVSLANAVVLPMVLKSALELNIIDIISTPGNSRGSLSASEIAQRIPAARNQDAPILLDRMLRVLASYDIVKCSSSTKENGQVERWYGAGPICKFLTKNQDGSGSAGPLLLMLHDEVLMKSWFHLNDAILEGGFPFSRAHGMTAFEYLGTDQRFNTLFNQAMSSYTTLVVKKILDVYRGFDGLNVLVDVGGGTGVTLSIIASKYPHIKGINYDLPHVLANSTSYSGVEHVKGDMFTSVPKGDAIFLKWILHDWSDEHCLKLLKNCWEALPSNGKVIVVECILPMAPENIVSSHFVHKQDLLMLTQSPGGKERTPKEFEALALKSGFSSCEAICCAYNSWVLEFHK
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University