Enzyme
The enzyme catalyses the biosynthesis of pterostilbene from resveratrol.
ROMT
Enzyme
Trans-resveratrol di-O-methyltransferase
Enzyme name
EC:2.1.1.240
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:trans-resveratrol 3,5-O-dimethyltransferase
Sysname
2 S-adenosyl-L-methionine + trans-resveratrol = 2 S-adenosyl-L-homocysteine + pterostilbene (overall reaction) [RN:R09872];(1a) S-adenosyl-L-methionine + trans-resveratrol = S-adenosyl-L-homocysteine + 3-methoxy-4',5-dihydroxy-trans-stilbene [RN:R09870];(1b) S-adenosyl-L-methionine + 3-methoxy-4',5-dihydroxy-trans-stilbene = S-adenosyl-L-homocysteine + pterostilbene [RN:R09871]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];pterostilbene [CPD:C10287];3-methoxy-4',5-dihydroxy-trans-stilbene [CPD:C20154]
Product
Species information
Manihot esculenta
Species name
Cassava (Manihot esculenta) is a species of eudicot in the family Euphorbiaceae (spurge family).
Species info
Gene sequence info
Manihot_esculenta.ROMT.fa
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>rna-XM_021776826.1
MRSLADGKQNAELLEAQSHIWNHIFNFINSMSLKSAVQLGIPDAIHSHGRPITISELIAALSLHPAKANCIPRLMRILVHSGFFARVKINQNDQEEGYILTNASQLLLKDHPLSVSPFLLAMLDPSLTRPWHYVRNWFQNDDPTPFATANGRTIWDFAGHEPEFNNLFNEAMASDARLAMNVLMNDCKGVFEGLRSLVDVGGGTGTVAKAIAKEFPQLECFVFDLPHVVAGLHGTHNLKYVGGSMFEAIPPADAILLKWIMHDWSHENCVKILKRCKEAIKGREGGKLIIIDMVMEQKEKEDHESKETELLFDMLMMVLYNSQERNEKEWAQLFSDAGFSNYKIISMLGLRSIIEVYP
>rna-XM_021776558.1
MINLGDAELLEAQAHVWNHIFNFINSMSLKCAVQLGIPDVIQRHGKPISLSHLISALPVHPAKSRCIPRLMRILVRSGFFARAKISENDEEEGYVLTNASQLLLKDNPLSVAPFLMAMLDPILTGPWHYMSTWFLNDDVTPFNTAHGKTFWEYHGHEPNLNNFFNEAMASDARLVTRVLINEFKGVFEGLKSLVDVGGGTGTVAKAIAKSFPDLDCTVFDLPHVVAGLQGTHNLKYFGGDMFDEIPPTDAILLKSILHDWSDEECVKILKRCKEAIKGRGGKLIIIDMMIENHKRDDDFPETQLFFDMLMMVLLTGKERNEKEWAKLFSDAGFSYHKISPVLGLRSIIEVYP
>rna-XM_021776847.1
MEFISEESIDELIQVQTHIWNHTFSFAKSMSLRCAVQLGIPDAINSHGRSMTLSELVAALPVHPTKTHHLHRLMRLLVHLGFFTSEKVAGDNHFQESYSLTPASRLLLKDNPFTYKHFIDLVLGPYLTKPYDSMSTWFQNDDSSPFLTEFGETLWDKAGHESELNRMINASMGGDSSLIGKAVIVKCKEVFEGLTSLVDVAGGTGNMAKAIADAFPHLNCTVFDQPRVVGDLQGSKNLNFVGGDMFKAVPPADAVLLKWTLHDWRDEDCVKILKNCKEAITIRNEKQGGKVMIIDMVVGDQTSDADDKITESKLLFDITAMSCLTGKQRNEKEWANLFFSAGFSSYKINPVLGPRALIEVYP
>rna-XM_021776846.1
MELVGGETVDELLEAQTHIWNNTFHIFKSMALKCAVQLGIPDAVNSHGKAMTLSELLAALPVHPTKTHHLYRLMRLLVHMGFFTLQKTADQEGYLLTPASRLLLKDNPLSTSSFVLIALDQVLLETYNCMSTWFQKDEPNPFVTVFGEPVWEYASHEARVNNLFNGAMANDSSLIGKAVVVKCKEVFQGLNSLVDVAGGTGNMAKAISDAFPDLKCTVLDLPHVVTDLEGNKNLNFLAGDMFKAVPPADAILLKWILHDWPDEECVKLLKNCKEAVRKNENEQQTGKVIIIDMVMENQAWNRTEVDDKITEAQLLLDMEMMCCVIGKERNEKEWAKLFFDAGFSNYKINHVLGSRALIEVYP
>rna-XM_021778135.1
MMELVSGETIDELLEAQTHIWNNTFHIFKSMALKCAVQLGIPNAVNSHGKAMTLSELVAALPVHPTKTHHFYRLMRLLVHMGFFTLEKTADQEGYLLTPASRLLLKNNPLNTSSYVFFVLDQVLLETFNCMSNWFQKDEPNPFVTVFGEPLWEYASHEARVNNLFNGAMSNDSSLIGKAVVVKCKEVFQGLNSLVDVAGGTGNMAKAISDAFPNLKCTVLDLPHVVADLEGNKNLNFLAGDMFKAVPPADAILLKWILHDWPDEECVKILKNCKEAIRKNGNEQQTGKIIIIDMVMGNQTWNSTKDDDKITEVQLLFDMEMMCRVIGKERNEKEWAKLFSDAGFSNYKIISVLGSRTLIEVYP
>rna-XM_021776730.1
MCSSKNKIKTMEFSDGESRNYLLEAQAHIWNHTFNFIKSMSLKCAVELHIPDIIHSHGQPMTLSELISALQINPSKSHHINRLMRILVHSGFFSLQKLGDNRHENGYLLTKTSLLLLKDHPQSARPHVLAALDPILTEPSHFLSSWFQNDDHPTPFSIAHGETFWDFASHEAKFNSSFNEAMASDSSLVIEVLVNDSKCKEVFKGLNSLVDVGGSTGIMAKAISEKFPNMKCTVLDLPHVVAGLQNDKNLNFLAGNMFDYIPPADAVLLKMVFHIMEDEECLKLLKKCREAVIGKGERPGKVIIIDIVMGDENCVKKSSETIELYDLDMMVLVSGKERNEEEWAKLFFEAGFSSYKINPVLGLRAIIEVYA
>rna-XM_021746581.1
MMELVGGETVDELLEAQTHIWNNTFHIFKSMALKCAVQLGIPDAVNSHGKAMTLSELVAALPVHPTKTHHFYRLMRLLVHMGFFTLQKTADQEGYLLTPASRLLLKDNPLSTSSFVLIALDQVLLETYNCMSTWFQKDEPNPFVTVFGEPLWEYASHEARVNNLFNGAMANDSSLIGKAVVVKCKEVFQGLNSLVDVAGGTGNMTKAISDAFPDLKCTVLDLPHVVTDLEGNKNLNFLAGDMFKAVPPADAILLKWILHDWPDEECVKILKNCKETIRKNKNEEQTGKVIIIDMVMGNQTWNSTKDDDNITEAQLLFDMEMMCSVIGKERNEKEWAKLFFDSGFSNYKINYVLGSRALIEVYP
>rna-XM_021746586.1
MINLGDAELLEAQAHVWNHIFNFINSMSLKCAVQLGIPDVIQRHGKPISLSHLISALPVHPAKSRCIPRLMRILVHSGFFARAKISENDEEEGYVLTNASQLLLKDNPLSVTPFLMAMLDPILTGPWHYMSTWFLNDDVTPFNTAHGKTFWEYLGHEPNLNNFFNEAMASDARLVTQVLINEFKGVFEGLKSLVDVGGGTGTLAKAIAKSFPDLDCTVFDLPHVVAGLQGTHNLKYVGGDMFDEIPPTDAILLKWILHDWSDEECVKILKRCKEAIKGRGGKLIIIDMMIENHNMVESCSKFAKVVCVARKGKVVAEPESSALGASASAPAFKPPTIVCTPSESAPANVGVEFSWPSSIQCLALMLNRVPSLLYDPTLAVLLTKSALRLEDRHSLNELETYELFDNVMHSALNSALYTYVAKEHHKTLRQEFGTQEADRRLLVKECSRLKTRVDKVEGTMKETLELVDKL
>rna-XM_021746583.1
MVNLGDAELLEAQAHVWNHIFNFINSMSLKCAVQLGIPDVIQRHGKPISLSHLISALPVHPAKSRCIPRLMRILVHSGFFARAKISENDEEEGYVLTNASQLLLKDNPFSVAPFLMAMLDPILIGPWHYMSTWFLNDDVTPFNTAHGKTFWEYHGHEPNLNNFFNEAMASDARLVTRVLINECKGVFEGLKSLVDVGGGTGTVAKAIAKSFPDLDCTVFDLPHVVAGLQGTHNLKYVGGDMFDEIPPSDAILLKWILHDWSDEECVKILKRCKEAIKGRKGGKLIIIDMIVGSHNGDDDSTETQLSFDMLMMVLVTGRERNEKEWAKLFSDAGFSYHKISPVLGLRSIIEVYP
>rna-XM_021746584.1
MINLGDAELLEAQAHVWNHIFNFINSMSLKCAVQLGIPDVIHRHGKPISLSQIISTLPVHPAKARCIPRLMRILVHSGFFARAKISENDDEEEGYVLTNASQLLLKDNPLSVTPFLIAMLDPILTGPWHYMSTWFLNDDVTPFDTAHAKTFWEYCGHEPNLNNFFNKAMASDARLITRVLINECKGVFEGLKSLVDVGGGTGTVAIAIAKAFPDLDCTVFDLPHVVADLEGTQNLEYIGGDMFEEIPPSDAILLKWILHSWNDEECVKILKRCKEAIKGRKGGKLIIIDMIVGSHNRDDDSTETQLSFDMLMMVLVTGRERNEKEWAKLFSDAGFSYHKINPVLGLRSIIEVYP
MRSLADGKQNAELLEAQSHIWNHIFNFINSMSLKSAVQLGIPDAIHSHGRPITISELIAALSLHPAKANCIPRLMRILVHSGFFARVKINQNDQEEGYILTNASQLLLKDHPLSVSPFLLAMLDPSLTRPWHYVRNWFQNDDPTPFATANGRTIWDFAGHEPEFNNLFNEAMASDARLAMNVLMNDCKGVFEGLRSLVDVGGGTGTVAKAIAKEFPQLECFVFDLPHVVAGLHGTHNLKYVGGSMFEAIPPADAILLKWIMHDWSHENCVKILKRCKEAIKGREGGKLIIIDMVMEQKEKEDHESKETELLFDMLMMVLYNSQERNEKEWAQLFSDAGFSNYKIISMLGLRSIIEVYP
>rna-XM_021776558.1
MINLGDAELLEAQAHVWNHIFNFINSMSLKCAVQLGIPDVIQRHGKPISLSHLISALPVHPAKSRCIPRLMRILVRSGFFARAKISENDEEEGYVLTNASQLLLKDNPLSVAPFLMAMLDPILTGPWHYMSTWFLNDDVTPFNTAHGKTFWEYHGHEPNLNNFFNEAMASDARLVTRVLINEFKGVFEGLKSLVDVGGGTGTVAKAIAKSFPDLDCTVFDLPHVVAGLQGTHNLKYFGGDMFDEIPPTDAILLKSILHDWSDEECVKILKRCKEAIKGRGGKLIIIDMMIENHKRDDDFPETQLFFDMLMMVLLTGKERNEKEWAKLFSDAGFSYHKISPVLGLRSIIEVYP
>rna-XM_021776847.1
MEFISEESIDELIQVQTHIWNHTFSFAKSMSLRCAVQLGIPDAINSHGRSMTLSELVAALPVHPTKTHHLHRLMRLLVHLGFFTSEKVAGDNHFQESYSLTPASRLLLKDNPFTYKHFIDLVLGPYLTKPYDSMSTWFQNDDSSPFLTEFGETLWDKAGHESELNRMINASMGGDSSLIGKAVIVKCKEVFEGLTSLVDVAGGTGNMAKAIADAFPHLNCTVFDQPRVVGDLQGSKNLNFVGGDMFKAVPPADAVLLKWTLHDWRDEDCVKILKNCKEAITIRNEKQGGKVMIIDMVVGDQTSDADDKITESKLLFDITAMSCLTGKQRNEKEWANLFFSAGFSSYKINPVLGPRALIEVYP
>rna-XM_021776846.1
MELVGGETVDELLEAQTHIWNNTFHIFKSMALKCAVQLGIPDAVNSHGKAMTLSELLAALPVHPTKTHHLYRLMRLLVHMGFFTLQKTADQEGYLLTPASRLLLKDNPLSTSSFVLIALDQVLLETYNCMSTWFQKDEPNPFVTVFGEPVWEYASHEARVNNLFNGAMANDSSLIGKAVVVKCKEVFQGLNSLVDVAGGTGNMAKAISDAFPDLKCTVLDLPHVVTDLEGNKNLNFLAGDMFKAVPPADAILLKWILHDWPDEECVKLLKNCKEAVRKNENEQQTGKVIIIDMVMENQAWNRTEVDDKITEAQLLLDMEMMCCVIGKERNEKEWAKLFFDAGFSNYKINHVLGSRALIEVYP
>rna-XM_021778135.1
MMELVSGETIDELLEAQTHIWNNTFHIFKSMALKCAVQLGIPNAVNSHGKAMTLSELVAALPVHPTKTHHFYRLMRLLVHMGFFTLEKTADQEGYLLTPASRLLLKNNPLNTSSYVFFVLDQVLLETFNCMSNWFQKDEPNPFVTVFGEPLWEYASHEARVNNLFNGAMSNDSSLIGKAVVVKCKEVFQGLNSLVDVAGGTGNMAKAISDAFPNLKCTVLDLPHVVADLEGNKNLNFLAGDMFKAVPPADAILLKWILHDWPDEECVKILKNCKEAIRKNGNEQQTGKIIIIDMVMGNQTWNSTKDDDKITEVQLLFDMEMMCRVIGKERNEKEWAKLFSDAGFSNYKIISVLGSRTLIEVYP
>rna-XM_021776730.1
MCSSKNKIKTMEFSDGESRNYLLEAQAHIWNHTFNFIKSMSLKCAVELHIPDIIHSHGQPMTLSELISALQINPSKSHHINRLMRILVHSGFFSLQKLGDNRHENGYLLTKTSLLLLKDHPQSARPHVLAALDPILTEPSHFLSSWFQNDDHPTPFSIAHGETFWDFASHEAKFNSSFNEAMASDSSLVIEVLVNDSKCKEVFKGLNSLVDVGGSTGIMAKAISEKFPNMKCTVLDLPHVVAGLQNDKNLNFLAGNMFDYIPPADAVLLKMVFHIMEDEECLKLLKKCREAVIGKGERPGKVIIIDIVMGDENCVKKSSETIELYDLDMMVLVSGKERNEEEWAKLFFEAGFSSYKINPVLGLRAIIEVYA
>rna-XM_021746581.1
MMELVGGETVDELLEAQTHIWNNTFHIFKSMALKCAVQLGIPDAVNSHGKAMTLSELVAALPVHPTKTHHFYRLMRLLVHMGFFTLQKTADQEGYLLTPASRLLLKDNPLSTSSFVLIALDQVLLETYNCMSTWFQKDEPNPFVTVFGEPLWEYASHEARVNNLFNGAMANDSSLIGKAVVVKCKEVFQGLNSLVDVAGGTGNMTKAISDAFPDLKCTVLDLPHVVTDLEGNKNLNFLAGDMFKAVPPADAILLKWILHDWPDEECVKILKNCKETIRKNKNEEQTGKVIIIDMVMGNQTWNSTKDDDNITEAQLLFDMEMMCSVIGKERNEKEWAKLFFDSGFSNYKINYVLGSRALIEVYP
>rna-XM_021746586.1
MINLGDAELLEAQAHVWNHIFNFINSMSLKCAVQLGIPDVIQRHGKPISLSHLISALPVHPAKSRCIPRLMRILVHSGFFARAKISENDEEEGYVLTNASQLLLKDNPLSVTPFLMAMLDPILTGPWHYMSTWFLNDDVTPFNTAHGKTFWEYLGHEPNLNNFFNEAMASDARLVTQVLINEFKGVFEGLKSLVDVGGGTGTLAKAIAKSFPDLDCTVFDLPHVVAGLQGTHNLKYVGGDMFDEIPPTDAILLKWILHDWSDEECVKILKRCKEAIKGRGGKLIIIDMMIENHNMVESCSKFAKVVCVARKGKVVAEPESSALGASASAPAFKPPTIVCTPSESAPANVGVEFSWPSSIQCLALMLNRVPSLLYDPTLAVLLTKSALRLEDRHSLNELETYELFDNVMHSALNSALYTYVAKEHHKTLRQEFGTQEADRRLLVKECSRLKTRVDKVEGTMKETLELVDKL
>rna-XM_021746583.1
MVNLGDAELLEAQAHVWNHIFNFINSMSLKCAVQLGIPDVIQRHGKPISLSHLISALPVHPAKSRCIPRLMRILVHSGFFARAKISENDEEEGYVLTNASQLLLKDNPFSVAPFLMAMLDPILIGPWHYMSTWFLNDDVTPFNTAHGKTFWEYHGHEPNLNNFFNEAMASDARLVTRVLINECKGVFEGLKSLVDVGGGTGTVAKAIAKSFPDLDCTVFDLPHVVAGLQGTHNLKYVGGDMFDEIPPSDAILLKWILHDWSDEECVKILKRCKEAIKGRKGGKLIIIDMIVGSHNGDDDSTETQLSFDMLMMVLVTGRERNEKEWAKLFSDAGFSYHKISPVLGLRSIIEVYP
>rna-XM_021746584.1
MINLGDAELLEAQAHVWNHIFNFINSMSLKCAVQLGIPDVIHRHGKPISLSQIISTLPVHPAKARCIPRLMRILVHSGFFARAKISENDDEEEGYVLTNASQLLLKDNPLSVTPFLIAMLDPILTGPWHYMSTWFLNDDVTPFDTAHAKTFWEYCGHEPNLNNFFNKAMASDARLITRVLINECKGVFEGLKSLVDVGGGTGTVAIAIAKAFPDLDCTVFDLPHVVADLEGTQNLEYIGGDMFEEIPPSDAILLKWILHSWNDEECVKILKRCKEAIKGRKGGKLIIIDMIVGSHNRDDDSTETQLSFDMLMMVLVTGRERNEKEWAKLFSDAGFSYHKINPVLGLRSIIEVYP
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University