Enzyme
The enzyme from alfalfa can methylate daidzein, genistein and 6,7,4'-trihydroxyisoflavone but not flavones or flavanones.
D7OMT
Enzyme
Isoflavone 7-O-methyltransferase
Enzyme name
EC:2.1.1.150
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:hydroxyisoflavone 7-O-methyltransferase
Sysname
S-adenosyl-L-methionine + a 7-hydroxyisoflavone = S-adenosyl-L-homocysteine + a 7-methoxyisoflavone [RN:R07375]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];7-methoxyisoflavone [CPD:C15616]
Product

Species information
Amphicarpaea edgeworthi
Species name
Chinese hog-peanut (Amphicarpaea edgeworthii) is a species of eudicot in the family Fabaceae (pea family).
Species info

Gene sequence info
Amphicarpaea_edgeworthi.D7OMT.fa
Download
>Ae.00020425
MDPSGTSNGRSLKTLPQQIEENSVRATFELTREDLEKIKKRWILHNWIHKDCIKILKNCKEAISNNGKRGKVIIIDVVINEKQEEHKVTELKLVMDITMACVNGKERNEEEWKKLFIEAGFQDYKISPLTGYLSLIEIYP
>Ae.00020506
MASNNGRKASEIFEGQHLLYRHMYAHVDSMILKCIVQLGIPDIIHNHGQPITLPKLVSILQVPPNKVSGLQSLMSYLAHNGFFEIVRIHDNNNNNIEEKELAYALTAASELLVKGSDLCLAPMVECFLDPTFSSSWHHLKKWIYEDDLTLFGISLGSHLWDFLDKNPAFNKSVNEAMASDSQMMNLALRDCNWVFEGLESIVDVGGGTGTTAKIICEAFPNLKCRVLELPHVIENLSGSNNLTYVAGDMFKSIPNADAWILHDWNDSDCRKILKNCKEAISSKSKRGKVIVIDVVINENQDEHEVTRLKLLMNVHMTCLINGKERREEEWKKLFVEAGLQSYKISPLTGHLSLIEIYP
>Ae.00020513
MAKEGKIIIIDIVINEKQDEHKVNELKLLWDVTMACVLNGKERNEEEWKTPFMEAGFQDYKISLLTGLSCMLPNLGFCPNTYETKLEKAICTLGHGNQDWHRVIDKDYLELELVNFERRSVVRISDPSTH
>Ae.00020302
MASSSLNGRNASEIFQGQTLLYRYVFSHIDSMCLKCIVELGIPDIIHKHDQPITLSELVSILKVPSARVGHVQSLMRYMAHNGFFERVRIHEEKEAYALTAASELLVKSSDLCLTPMVDFVLDPTLLGSFQQMNKWVYEEDLSVHDISLGSNLWDFLNKNPAYNELFNEWMASDSQISNFALRDCKLVFEGLESIVDVGGGTGATVKIICQAFPNMKCIVFDRPQVVENLSGNNSFTYVGGDMFKSIPKADAILLKMILHNWNDDDCRKILENCKEAILGKGKIGKIIVIDVVINENQDEREVTGLKLLMNLNMACVFNGKERREEEWKKLFVEAGFQSYNISPLTAHFSLIEIYP
>Ae.00020372
MVSNNGRKASEIFQGQALLYRYVFAFVDSMRLKWIVELYIPDIIHNHGQPITLSELVSILQVPPARFGHVQNLMRYMAHNGFFERVRIHEEKEAYSLTAASELLVKSSDLCLTPMVEHILDPTLSASFHQMKKGSNLWDFLDKNPANNELFNEAMARDSQMSNLALRDCKSVFEGLESIVDVGGGTGATAKMICEAFPDLKCTVLDRPQVVENLKKATI
>Ae.00012172
MASMKSNQEAIELFEGQNLLYMPIWQPKTYVHNTQPCQTLPELVSTLQIPPTKASFVHRIMRFLAYNGIFDIHESQKEDHELVYALTPASKLLVNGSGHCLSPMVLLLTDPLLMSRFHYLGEWIRGKEDAIVYETASGTTLWGLLEEKPTYLSLFSEGMASDSQMVDLALKNCTSVFEELDSMVDCILHDWNDENCIKILQKCKDSISSKGNRGKVIIIDAK
>Ae.00012221
MGSMNNNQNENELLEGQALLYMQLFAYLRPMCLKWVVQLGIPDIIQNHAKPITLSELVSTLQIPPAKACLVQRFMRFLAHNGIFDIHESQKEDHELAYALTPASKLLVSGSDHCLSPMVLLNTDPLVTSTYHHLGEWIRGEDPTLYETALGTTIWGLFEEKPTYMSLFNEAMASDSQMVDLALKNCTSVFEELDSIVDVGGGTGTTARIICEAFPKLKCVVLDLPHVVANLKGSNNLSFVGGDMFNSIPQADAILLKWVLHDWNDENCIKILQQCRDSIVSKGSIGKVIIIDAVINEKLDDRNMTQTKLSLDIIMLTMNGRERTENEWKQLFIEAGFKHHKIFPLFGFRSLIEVFP
>Ae.00012477
MGSNSMNNQNAIEFFHGQALLYTQIYGCLKPMCLKWVVQLGIPDIIHNHGKPITLSELVSTLQIPPAKANFVQRFMRFLAHNGIFDIRESQEEDHESTYALTPASKLLVSGSDHCLSPMVLFNTDSAVTNTYHHFENWIRREDTTLYETAVGTTMWGYLEEKPKYLSHFNEAMASDSRIVDLALKNCTSVFEGLDSIVDVGGGTGTTAKIICETFPKLKCVVFDLPHVVANLTGNDNLSFVGGDMFNFIPQADAVLLKWVLHNWNDEYCIMILKKCKDSISNKGTGGKVVIIDAVINEKLDDPDMIQAKITFDIIMMTMNGRERTEKEWKQLFIEAGFKHYKIFPIFGFRSLIEVYP
MDPSGTSNGRSLKTLPQQIEENSVRATFELTREDLEKIKKRWILHNWIHKDCIKILKNCKEAISNNGKRGKVIIIDVVINEKQEEHKVTELKLVMDITMACVNGKERNEEEWKKLFIEAGFQDYKISPLTGYLSLIEIYP
>Ae.00020506
MASNNGRKASEIFEGQHLLYRHMYAHVDSMILKCIVQLGIPDIIHNHGQPITLPKLVSILQVPPNKVSGLQSLMSYLAHNGFFEIVRIHDNNNNNIEEKELAYALTAASELLVKGSDLCLAPMVECFLDPTFSSSWHHLKKWIYEDDLTLFGISLGSHLWDFLDKNPAFNKSVNEAMASDSQMMNLALRDCNWVFEGLESIVDVGGGTGTTAKIICEAFPNLKCRVLELPHVIENLSGSNNLTYVAGDMFKSIPNADAWILHDWNDSDCRKILKNCKEAISSKSKRGKVIVIDVVINENQDEHEVTRLKLLMNVHMTCLINGKERREEEWKKLFVEAGLQSYKISPLTGHLSLIEIYP
>Ae.00020513
MAKEGKIIIIDIVINEKQDEHKVNELKLLWDVTMACVLNGKERNEEEWKTPFMEAGFQDYKISLLTGLSCMLPNLGFCPNTYETKLEKAICTLGHGNQDWHRVIDKDYLELELVNFERRSVVRISDPSTH
>Ae.00020302
MASSSLNGRNASEIFQGQTLLYRYVFSHIDSMCLKCIVELGIPDIIHKHDQPITLSELVSILKVPSARVGHVQSLMRYMAHNGFFERVRIHEEKEAYALTAASELLVKSSDLCLTPMVDFVLDPTLLGSFQQMNKWVYEEDLSVHDISLGSNLWDFLNKNPAYNELFNEWMASDSQISNFALRDCKLVFEGLESIVDVGGGTGATVKIICQAFPNMKCIVFDRPQVVENLSGNNSFTYVGGDMFKSIPKADAILLKMILHNWNDDDCRKILENCKEAILGKGKIGKIIVIDVVINENQDEREVTGLKLLMNLNMACVFNGKERREEEWKKLFVEAGFQSYNISPLTAHFSLIEIYP
>Ae.00020372
MVSNNGRKASEIFQGQALLYRYVFAFVDSMRLKWIVELYIPDIIHNHGQPITLSELVSILQVPPARFGHVQNLMRYMAHNGFFERVRIHEEKEAYSLTAASELLVKSSDLCLTPMVEHILDPTLSASFHQMKKGSNLWDFLDKNPANNELFNEAMARDSQMSNLALRDCKSVFEGLESIVDVGGGTGATAKMICEAFPDLKCTVLDRPQVVENLKKATI
>Ae.00012172
MASMKSNQEAIELFEGQNLLYMPIWQPKTYVHNTQPCQTLPELVSTLQIPPTKASFVHRIMRFLAYNGIFDIHESQKEDHELVYALTPASKLLVNGSGHCLSPMVLLLTDPLLMSRFHYLGEWIRGKEDAIVYETASGTTLWGLLEEKPTYLSLFSEGMASDSQMVDLALKNCTSVFEELDSMVDCILHDWNDENCIKILQKCKDSISSKGNRGKVIIIDAK
>Ae.00012221
MGSMNNNQNENELLEGQALLYMQLFAYLRPMCLKWVVQLGIPDIIQNHAKPITLSELVSTLQIPPAKACLVQRFMRFLAHNGIFDIHESQKEDHELAYALTPASKLLVSGSDHCLSPMVLLNTDPLVTSTYHHLGEWIRGEDPTLYETALGTTIWGLFEEKPTYMSLFNEAMASDSQMVDLALKNCTSVFEELDSIVDVGGGTGTTARIICEAFPKLKCVVLDLPHVVANLKGSNNLSFVGGDMFNSIPQADAILLKWVLHDWNDENCIKILQQCRDSIVSKGSIGKVIIIDAVINEKLDDRNMTQTKLSLDIIMLTMNGRERTENEWKQLFIEAGFKHHKIFPLFGFRSLIEVFP
>Ae.00012477
MGSNSMNNQNAIEFFHGQALLYTQIYGCLKPMCLKWVVQLGIPDIIHNHGKPITLSELVSTLQIPPAKANFVQRFMRFLAHNGIFDIRESQEEDHESTYALTPASKLLVSGSDHCLSPMVLFNTDSAVTNTYHHFENWIRREDTTLYETAVGTTMWGYLEEKPKYLSHFNEAMASDSRIVDLALKNCTSVFEGLDSIVDVGGGTGTTAKIICETFPKLKCVVFDLPHVVANLTGNDNLSFVGGDMFNFIPQADAVLLKWVLHNWNDEYCIMILKKCKDSISNKGTGGKVVIIDAVINEKLDDPDMIQAKITFDIIMMTMNGRERTEKEWKQLFIEAGFKHYKIFPIFGFRSLIEVYP
Gene tree
Gene tree

Sheng jun & Dong yang Teams, Yunnan agriculture University