Enzyme
The enzyme catalyses the synthesis of catechin, catechin-4beta-ol (leucocyanidin) and the related flavan-3-ols afzelechin and gallocatechin, which are initiating monomers in the synthesis of plant polymeric proanthocyanidins or condensed tannins. While 2,3-trans-3,4-cis-leucocyanidin is the preferred flavan-3,4-diol substrate, 2,3-trans-3,4-cis-leucodelphinidin and 2,3-trans-3,4-cis-leucopelargonidin can also act as substrates, but more slowly. NADH can replace NADPH but is oxidized more slowly.
LAR1
Enzyme
Leucoanthocyanidin reductase
Enzyme name
EC:1.17.1.3
Entry
Oxidoreductases;Acting on CH or CH2 groups;With NAD+ or NADP+ as acceptor
Class
(2R,3S)-catechin:NADP+ 4-oxidoreductase
Sysname
(2R,3S)-catechin + NADP+ + H2O = 2,3-trans-3,4-cis-leucocyanidin + NADPH + H+ [RN:R06532]
Reaction
2,3-trans-3,4-cis-leucocyanidin [CPD:C05906];NADPH [CPD:C00005];H+ [CPD:C00080]
Product
Species information
Malus domestica
Species name
Apple (Malus domestica) is a species of eudicot in the family Rosaceae (rose family).
Species info
Gene sequence info
Malus_domestica.LAR1.fa
Download
>rna-XM_008381885.3
MLPIEMLGHGEDTPSRFELLSMVKKHSSLLGKTVIVADEDDTSDVELDHRFWHDVLDFYFVRGKDSRGRQDDDLVFFVRKLISYGYGFSDGKEGVSPYFVRRWAPKLDDLIGESNVDVDWRRTFYLNLIAHTSFTVTVAICSHQDLRNHQAQQGIPLSPIYKVVKTVYASPSRVNFQLDSKKEVETTPAYPDICFAIDDFDSTFDAVVLTETDHCYCVILNAHDGAAFPREKESNDCSPSDSSSLTVETNSAQMKNTKLTLFSGFVSYQMVRDAYDAGKSRFGNLLGHSPGKTDKLYMKGPGGRGEVEVAVSRVADQSQQDLGPFSPVISKRGFGIGSIVRKAASVASVAAKNAYAAASSTYSFDDEMVPLKCCLMSISLPWEQIAYDLLFKGTPPVTL
>rna-NM_001293860.1
MTVSPSLSVARNGRVLIVGATGFIGRFVAEASLAAGRPTYVLVRPGPLHPSKADTVKSFKHKGAIILHGLISDKTLMEKILREHEIETVISAVGGATILDQIALVEAIAAVGTVKRFLPSEFGHDVDRADPVEPGLTMYEEKRKVRRWVEKSGVAYTYICCNSIASWPYFDNTHPSEVLPPLDRFQIYGDGTVKAYFVDGTDIGKFTIKTVDDIRTINKNIHFRPPSNLYDINGLASLWEKKIGRTLPRITITENHLLALAAENRIPESIVASFTHDIFIKGCQVNFAVEGPRDVEVGTLYPGDSFRTLDECFNDFLLKLKDNLEPVHEENVSTKNAVVESRAVTPTCA
>rna-XM_029094583.1
MLGHGEETPSRFELLSMVKKHSNLLGKTVIVADEDDTSDVELDHRFWHDMLDLYFVRGKDSRGRQDDDLVFFVRKLSYYGYGFNDGKEGVSPYFVRRWAHKLDDLIGESNVDVDWRRSFYLNLIAHTSFTVTVAICSHQDLRNHQAEQGIPLSPIYKVVKTVYASPSRVDFQLDSKKEVETTPAYPDICFAIDDFDSTFDAVVLTETDHCYCVILNAHDGAAFPRVREVDECSSSDSSSLRIETNSAKTKNTKLTLFSGFVSYQMVRDAYDAGKSRFGNLLGHSPGKTDKLYMKGPGGRGEVEVAVSGVADQSQQDLGPFSPVISKTGFGIGSIVRKAASVASVAAKNAYAAASSTHSFDDEMVPLKCCLMSISLPWEQIAHDLLFKGTPPVTL
>rna-XM_017323641.2
MTVSSSLSVAKSGSVLIVGATGFIEAIGEVGTVKRFLPSEFRHEVDRADPVEPGLTMYLEKRKVRRWVEESGVP
>rna-NM_001294114.1
MTVSSSLSVAKNGRVLIAGATGFIGRFVAEASLAAGQPTYVLVRPGPLHPSKADTVKSFKDKGAIILHGLISDKTLMEKMLREHEIEIVISAVGGATILDQITLVDAIAAVGTVKRFLPSEFGHDVDRADPVEPGLTMYLEKRKVRRSVETSGVPYTYICCNSIASWPYFDNTHPSEVLPPLDRFQIYGDGTVKAYFVDGTDIGKFTMKTVDDMRTINKNVHFRPPSNLYDINGLASLWEKKIGRTLPRVTVTEDHLLALAADNRIPESIVASFTHDIFIKGCQVNFAVEGPHDVEVGTLYPGDSFRTLDECFDGFLLKLKDNLELELLQEEEDQKVSTENTVVESRTVTATCA
MLPIEMLGHGEDTPSRFELLSMVKKHSSLLGKTVIVADEDDTSDVELDHRFWHDVLDFYFVRGKDSRGRQDDDLVFFVRKLISYGYGFSDGKEGVSPYFVRRWAPKLDDLIGESNVDVDWRRTFYLNLIAHTSFTVTVAICSHQDLRNHQAQQGIPLSPIYKVVKTVYASPSRVNFQLDSKKEVETTPAYPDICFAIDDFDSTFDAVVLTETDHCYCVILNAHDGAAFPREKESNDCSPSDSSSLTVETNSAQMKNTKLTLFSGFVSYQMVRDAYDAGKSRFGNLLGHSPGKTDKLYMKGPGGRGEVEVAVSRVADQSQQDLGPFSPVISKRGFGIGSIVRKAASVASVAAKNAYAAASSTYSFDDEMVPLKCCLMSISLPWEQIAYDLLFKGTPPVTL
>rna-NM_001293860.1
MTVSPSLSVARNGRVLIVGATGFIGRFVAEASLAAGRPTYVLVRPGPLHPSKADTVKSFKHKGAIILHGLISDKTLMEKILREHEIETVISAVGGATILDQIALVEAIAAVGTVKRFLPSEFGHDVDRADPVEPGLTMYEEKRKVRRWVEKSGVAYTYICCNSIASWPYFDNTHPSEVLPPLDRFQIYGDGTVKAYFVDGTDIGKFTIKTVDDIRTINKNIHFRPPSNLYDINGLASLWEKKIGRTLPRITITENHLLALAAENRIPESIVASFTHDIFIKGCQVNFAVEGPRDVEVGTLYPGDSFRTLDECFNDFLLKLKDNLEPVHEENVSTKNAVVESRAVTPTCA
>rna-XM_029094583.1
MLGHGEETPSRFELLSMVKKHSNLLGKTVIVADEDDTSDVELDHRFWHDMLDLYFVRGKDSRGRQDDDLVFFVRKLSYYGYGFNDGKEGVSPYFVRRWAHKLDDLIGESNVDVDWRRSFYLNLIAHTSFTVTVAICSHQDLRNHQAEQGIPLSPIYKVVKTVYASPSRVDFQLDSKKEVETTPAYPDICFAIDDFDSTFDAVVLTETDHCYCVILNAHDGAAFPRVREVDECSSSDSSSLRIETNSAKTKNTKLTLFSGFVSYQMVRDAYDAGKSRFGNLLGHSPGKTDKLYMKGPGGRGEVEVAVSGVADQSQQDLGPFSPVISKTGFGIGSIVRKAASVASVAAKNAYAAASSTHSFDDEMVPLKCCLMSISLPWEQIAHDLLFKGTPPVTL
>rna-XM_017323641.2
MTVSSSLSVAKSGSVLIVGATGFIEAIGEVGTVKRFLPSEFRHEVDRADPVEPGLTMYLEKRKVRRWVEESGVP
>rna-NM_001294114.1
MTVSSSLSVAKNGRVLIAGATGFIGRFVAEASLAAGQPTYVLVRPGPLHPSKADTVKSFKDKGAIILHGLISDKTLMEKMLREHEIEIVISAVGGATILDQITLVDAIAAVGTVKRFLPSEFGHDVDRADPVEPGLTMYLEKRKVRRSVETSGVPYTYICCNSIASWPYFDNTHPSEVLPPLDRFQIYGDGTVKAYFVDGTDIGKFTMKTVDDMRTINKNVHFRPPSNLYDINGLASLWEKKIGRTLPRVTVTEDHLLALAADNRIPESIVASFTHDIFIKGCQVNFAVEGPHDVEVGTLYPGDSFRTLDECFDGFLLKLKDNLELELLQEEEDQKVSTENTVVESRTVTATCA
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University