Enzyme
This plant enzyme, involved in the biosynthesis of anthocyanidins, is known to act on (+)-dihydrokaempferol, (+)-taxifolin, and (+)-dihydromyricetin, although some enzymes may act only on a subset of these compounds. Each dihydroflavonol is reduced to the corresponding cis-flavan-3,4-diol. NAD+ can act instead of NADP+, but more slowly.
DFR
Enzyme
Dihydroflavonol 4-reductase
Enzyme name
EC:1.1.1.219
Entry
Oxidoreductases;Acting on the CH-OH group of donors;With NAD+ or NADP+ as acceptor
Class
(2R,3S,4S)-leucoanthocyanidin:NADP+ 4-oxidoreductase
Sysname
a (2R,3S,4S)-leucoanthocyanidin + NADP+ = a (2R,3R)-dihydroflavonol + NADPH + H+ [RN:R11500]
Reaction
(2R,3R)-dihydroflavonol [CPD:C21417];NADPH [CPD:C00005];H+ [CPD:C00080]
Product
Species information
Malus domestica
Species name
Apple (Malus domestica) is a species of eudicot in the family Rosaceae (rose family).
Species info
Gene sequence info
Malus_domestica.DFR.fa
Download
>rna-XM_029099276.1
MDESCWTPLNLSLAFANKVLIDYTHSKSLSEKEVLKHTSGDLEVVSIACGLVGGDTLLTTMPESMGVLISQITKDSRRYQTLRFLEELLGKVPIVHIEDVCEAHILCMEKPSINGRFLCVSAYLSSAEIASHWEKNYPSLKIAEEFVEDSGRDIGWGSTKLKKIGFEYKFDAKVILDDTLKWAQKMGEFASSQNTVVPK
>rna-XM_008367559.3
MHISLCVYIYFCVCVSACGQKKGRAMATYCVTGATGFIGSWLVKSLLEKGHMVHATVRDPAKCLHLLSLWTSIDRLRLLRADLGEEGSFDEAVKGCDGVFHVAASMEFSVTVKENIESYVRSNIIEPAIKGTLDLLKSCVNSGTVKRVVLTSSISTITAKDGNGNARVVVDESCQTPVDQVLTAKANGWVYVLLKILTEEAAFKFASEKGINLVSVITSTVGGPFLTSKVPSSIQVLLSPVTGDSELYGILSAVNARMGSIALVHIEDTCNAHMFLMEHAKAEGRYVCVAQSCPISKLLNYLAQAYPCSNSNRYRVIAEENDVVPSEISSKKLTDLGFTYKHGIEDIVHQTVSCCLGYGFLPPI
>rna-XM_029103476.1
MSKILAEKAGWDFCKENAIDMVTVLPSFVIGPCLPPELCSTASDVLGLLKGETEKFKWNGRMGYVHIDDVALCHILVYEHESAHGRCICSSTVMDNTELGSLLSTRYPSLPIPTRFEQLGRPRYYELNTSKLGRGFKFKSIQEMFDDCIASLVEQGHLPAL
>rna-XM_008379159.3
MGSKSESVCVTGASGFIGSWLVMRLLERGYTVRATVRDPTNQKKVKHLLDLPKAETHLTLWKADLADEGSFDEAIQGCTGVFHVATPMDFESKDPENEVIKPTINGLLDILKACQKVKTVRRLVFTSSAGTVIMEEHKKPVYDESNWSDVEFCLSVKMTGWMYFVSKTLAEQAAWKYAKENNIDFITIIPTLVIGPFLMPSMPPSLITGLSPITRTKSHYGIIKQGQYVHLDDLCNSHIYLYEHPKAEGRYICSSHDATIHDLVKMLREKYPAYNIPTKFEGIDDNLDTIHFSSKKLRDIGFEFKYSFEDMFVGAVDTCRAKGLIPIPAEKTEAADESNVVDIKIAA
>rna-XM_008387812.3
MATYCVTGATGFIGSWLVKTLLEKGHMVHATVRDPAKCLQLLSLWTSTDRLRLFRADLRQEGSFDEAVKGCDGVFHVAASMEFSVTVTENIESYVQSNTIEPAIKGTIDLLQSCVNSGTVKRVVLTSSISTIIAKDGNGNARAVVDESCQTPVDQVLSAKASGWVYVLSKILTEEAAFRFASDNGINLVSVITSTVGGNFLTSKVPSSIQVLLSPITGDSELYGILSAVNARMGSIALVHVEDICNAHIFLMEHTKAEGRYICVAQSCPISKLLNYLAQAYPCSNSNRYRVIEEENDVVPYSEISSKKLTDLGFTYKHGIEDMVHQTVSCCVDYGFLPPI
>rna-NM_001293939.1
MGSESESVCVTGASGFIGSWLVMRLLEHGYTVRATVRDPTNQKKVKHLLDLPKAETHLTLWKADLADEGSFDEAIQGCSGVFHVATPMDFESKDPENEVIKPTINGLLDILKACQKAKTVRKLVFTSSAGTVNVEEHQKPVYDESNWSDVEFCRSVKMTGWMYFVSKTLAEQAAWKYAKENNIDFITIIPTLVIGPFLMPSMPPSLITGLSPILRNESHYGIIKQGQYVHLDDLCLSHIYLYEHPKAEGRYICSSHDATIHELVKMLREKYPEYNIPTKFKGIDDNLEPVHFSSKKLREIGFEFKYSLEDMFVGAVDACRAKGLIPIPIPAEKTEAAEESNLVDVKVG
MDESCWTPLNLSLAFANKVLIDYTHSKSLSEKEVLKHTSGDLEVVSIACGLVGGDTLLTTMPESMGVLISQITKDSRRYQTLRFLEELLGKVPIVHIEDVCEAHILCMEKPSINGRFLCVSAYLSSAEIASHWEKNYPSLKIAEEFVEDSGRDIGWGSTKLKKIGFEYKFDAKVILDDTLKWAQKMGEFASSQNTVVPK
>rna-XM_008367559.3
MHISLCVYIYFCVCVSACGQKKGRAMATYCVTGATGFIGSWLVKSLLEKGHMVHATVRDPAKCLHLLSLWTSIDRLRLLRADLGEEGSFDEAVKGCDGVFHVAASMEFSVTVKENIESYVRSNIIEPAIKGTLDLLKSCVNSGTVKRVVLTSSISTITAKDGNGNARVVVDESCQTPVDQVLTAKANGWVYVLLKILTEEAAFKFASEKGINLVSVITSTVGGPFLTSKVPSSIQVLLSPVTGDSELYGILSAVNARMGSIALVHIEDTCNAHMFLMEHAKAEGRYVCVAQSCPISKLLNYLAQAYPCSNSNRYRVIAEENDVVPSEISSKKLTDLGFTYKHGIEDIVHQTVSCCLGYGFLPPI
>rna-XM_029103476.1
MSKILAEKAGWDFCKENAIDMVTVLPSFVIGPCLPPELCSTASDVLGLLKGETEKFKWNGRMGYVHIDDVALCHILVYEHESAHGRCICSSTVMDNTELGSLLSTRYPSLPIPTRFEQLGRPRYYELNTSKLGRGFKFKSIQEMFDDCIASLVEQGHLPAL
>rna-XM_008379159.3
MGSKSESVCVTGASGFIGSWLVMRLLERGYTVRATVRDPTNQKKVKHLLDLPKAETHLTLWKADLADEGSFDEAIQGCTGVFHVATPMDFESKDPENEVIKPTINGLLDILKACQKVKTVRRLVFTSSAGTVIMEEHKKPVYDESNWSDVEFCLSVKMTGWMYFVSKTLAEQAAWKYAKENNIDFITIIPTLVIGPFLMPSMPPSLITGLSPITRTKSHYGIIKQGQYVHLDDLCNSHIYLYEHPKAEGRYICSSHDATIHDLVKMLREKYPAYNIPTKFEGIDDNLDTIHFSSKKLRDIGFEFKYSFEDMFVGAVDTCRAKGLIPIPAEKTEAADESNVVDIKIAA
>rna-XM_008387812.3
MATYCVTGATGFIGSWLVKTLLEKGHMVHATVRDPAKCLQLLSLWTSTDRLRLFRADLRQEGSFDEAVKGCDGVFHVAASMEFSVTVTENIESYVQSNTIEPAIKGTIDLLQSCVNSGTVKRVVLTSSISTIIAKDGNGNARAVVDESCQTPVDQVLSAKASGWVYVLSKILTEEAAFRFASDNGINLVSVITSTVGGNFLTSKVPSSIQVLLSPITGDSELYGILSAVNARMGSIALVHVEDICNAHIFLMEHTKAEGRYICVAQSCPISKLLNYLAQAYPCSNSNRYRVIEEENDVVPYSEISSKKLTDLGFTYKHGIEDMVHQTVSCCVDYGFLPPI
>rna-NM_001293939.1
MGSESESVCVTGASGFIGSWLVMRLLEHGYTVRATVRDPTNQKKVKHLLDLPKAETHLTLWKADLADEGSFDEAIQGCSGVFHVATPMDFESKDPENEVIKPTINGLLDILKACQKAKTVRKLVFTSSAGTVNVEEHQKPVYDESNWSDVEFCRSVKMTGWMYFVSKTLAEQAAWKYAKENNIDFITIIPTLVIGPFLMPSMPPSLITGLSPILRNESHYGIIKQGQYVHLDDLCLSHIYLYEHPKAEGRYICSSHDATIHELVKMLREKYPEYNIPTKFKGIDDNLEPVHFSSKKLREIGFEFKYSLEDMFVGAVDACRAKGLIPIPIPAEKTEAAEESNLVDVKVG
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University