Enzyme

In addition to the desaturation of (2R,3R)-dihydroflavonols to flavonols, the enzyme from Citrus unshiu (satsuma mandarin) also has a non-specific activity that trans-hydroxylates the flavanones (2S)-naringenin and the unnatural (2R)-naringenin at C-3 to kaempferol and (2R,3R)-dihydrokaempferol, respectively [2]. Requires Fe2+.

FLS

Enzyme

Flavonol synthase

Enzyme name

EC:1.14.20.6

Entry

Oxidoreductases;Acting on paired donors, with incorporation or reduction of molecular oxygen;With 2-oxoglutarate as one donor, and the other dehydrogenated

Class

dihydroflavonol,2-oxoglutarate:oxygen oxidoreductase

Sysname

a dihydroflavonol + 2-oxoglutarate + O2 = a flavonol + succinate + CO2 + H2O [RN:R07368]

Reaction

flavonol [CPD:C01495];succinate [CPD:C00042];CO2 [CPD:C00011];H2O [CPD:C00001]

Product

Species information

Magnolia biondii

Species name

Magnolia biondii is a species of flowering plant in the family Magnoliaceae (magnolia family).

Species info

Gene sequence info

Magnolia_biondii.FLS.fa

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>MBI05980_MAGBIO
MEVERVQSIASANLSKDNIPTEFIRLENEQPGTTTFYGPIPEIPIVDLSGKDHNRVVREIANASEEFGIFQVVNHGIPTDVIEKLQSVGKAFFQLREEEKEKYAIKPGSGKLEGYGTKLQKESEGKKAWVDFFFHNVWPPSSVNYDAWPRNPNYKEANEEYAKHMKGVVDSILANLSLGLGLEGGVLKDAVGGEDLEYLLKINYYPPCPRPDLALGVVAHTDMSAVTILVPNDVSGLQVFKDDIWFNVNYIPNALIVHIGDQIEILSNGKYKAVLHRSTVNKEKTRMSWPVFCSPPQEMFMGPLPQLVNDENPPRFKTKKYKDYQHCKLNKLPQ
>MBI26566_MAGBIO
MAINQIDNGDECSEFLLAIMGRKRWGLDGAKQWEVERVQSIASANLSKDNIPTEFIRLENEQPGTTTFYGPIPEIPIVDLSDKDHNRVVREIANASEEFGIFQVVNHGIPTDVIEKLQSVGKAFFQLPEEEKEKYAIKPGSGKLEGYGTKLQKESEGKKAWVDFFFHNVWPPSSVNYDAWPRNPNYKEANEEYAKHMKGVVDSILANLSLGLGLEGGVLKDAVGGEDLEYLLKINYYPPCPRPDLALGVVAHTDMSAVTILVPNDVSGLQVFKDDIWFNVNYIPNALIVHIGDQIEILSNGKYKAVLHRSTVNKEKTRMSWPVFCSPPQEMFMGPLPQLVNDENPPRFKTKKYKDYQHCKLNKLPQ
>MBI26565_MAGBIO
MSCRRSLCGPLTSSLLIPRPSKESRCPRSLYLNLIMLSRIKLLVHAGSGGFFVLTDHGISADLIRCLQDAGKEFFQLPQEEKEKYANDPTSGNMEGYGTKMTKNLEQKVEWIDYYFHLMTPPSRVNFKLWPTNPPSYREVTEEYGKEVMRVVDRLLELLSVDLGLERTALKATIGGDRIELEMKINFYPPCPQPELALGVEPHTDMSALTLLVQNDVPGLQLWKDGNWVTVDYSPDALFVHIGDQMEVLSNGKYKSILHRSLVSKGQKRMSWAVFCAPPGDAVIGPLPQLVGDDNPPLFNTKTYKEFQYRKFNKLPQ
>MBI05981_MAGBIO
MEVERVQSLASVGLNELPSQFVRPAHEQPANTKAVQGVKVPKISLSQPDHVVKDQVASACREWGFFVLTDHGISADLIRCLQDAGKEFFQLPQEEKEKYANDPTSGNMEGYGTKMTKNLEQKVEWIDYYFHLMTPPSRVNFKLCGLPILRPTEVTEEYGKEVMRVVDRLLELLSVDLGLERTALKATIGGDRIELEMKINFYPPCPQPELALGVEPHTDMSALTLLVQNDVPGLQLWKDGNWFTVDYSPDALFVHIGDQMEVLSNGKYKSILHRSLVSKDQKRMSWAVFCAPPGDALVGDDNPPLFNTKTYKEFQYRKFNKLPQKHAELHPG
>MBI02572_MAGBIO
MEVERVQAIASMFTAKDGIPAEFVRSENERPGITTFQGPVPDVPTIDLSDPNREKIIREIADASQEWGIFQVVNHGIPDDIIRKLQDVGKEFFGLPQEEKEEYAIKPESDTLEGYGTKLQKDMEGKKAWVDFFFHNIWPLSRINYNVWPKNPPAYREANEEYAKYMLNVVDDLLSSLSLGLGLEAGVLKDAVGGDDLEFLLKINYYPPCPRPDLALGVVAHTDMSAITVLVPNEISGLQVFKDNIWFDVKYIPNALIVHIGDQIEILSNGKYKSVLHRTTVNKEKTRMSWPVFCSPPAEMVVGPLSQFVDDRNPPKFKTKKYKDYQYCKLNKLPQ
>MBI11271_MAGBIO
MLNVVDDLLSSLSLGLGLEAGVLKDVVGGDDLEFLLKINYYPPCPWPDLALGVVAHTDMSAIIDLVPNEISGLQVFKDDIWFDIKYIPNALVVHIRDQIESLWKTTRQQVAELEEMRGLENLPCGPCI

Gene tree

Gene tree

Sheng jun & Dong yang Teams, Yunnan agriculture University