Enzyme
4CL
Enzyme
4-coumarate:CoA ligase
Enzyme name
EC:6.2.1.12
Entry
Ligases;Forming carbon-sulfur bonds;Acid-thiol ligases
Class
4-coumarate:CoA ligase (AMP-forming)
Sysname
ATP + 4-coumarate + CoA = AMP + diphosphate + 4-coumaroyl-CoA [RN:R01616]
Reaction
AMP [CPD:C00020];diphosphate [CPD:C00013];4-coumaroyl-CoA [CPD:C00223]
Product
Species information
Magnolia biondii
Species name
Magnolia biondii is a species of flowering plant in the family Magnoliaceae (magnolia family).
Species info
Gene sequence info
Magnolia_biondii.4CL.fa
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>MBI34385_MAGBIO
MLSVASPDTQPPTPAPEGPPPETFIFRSKLPDISLSNHLPLHTYCFEKASEFPDRPCLITGSNGRIYSYAEAHLICQKTAAGLSKLGIKKGDVIMILLQNSPEFVFSFMGASIIGAVTTAANPFCTAAEIYKQFSSSAAKLVITQSQYVNKLRDAGEKFPTIGDELPVITIDDPPENCLHFSVISSADEREVPSVSIEPDDPVALPFSSGTTGLPKGVILTHKSLISSVAQQIDGENPNLHLTTEDVVLCVLPLFHIFSLNSVLLCSLRAASGVLLMQKFEIGTLLDLIQRYRVSVAMVVPPLVLALAKNPTVENFDLSSIRIILSGAAPLGKELEDALRSRVPQALLGQGYGMTEAGPVLSMCLGFAKQPFPTKSGSCGTVVRNADLKVIDPETGFSLQHNQPGEICIRGPQVMKGYLNDPKATASTIDAEGWLHTGDIGYVDDDEEVYIVDRVKELIKFKGFQVPPAELEALLVSHPSIADAAVVPQKDVAAGEVPVAFIVQSNGYDLTEDAVKEFIAKQVVFYKRLHKVYFVHAIPKSPSGKILRKDLRAKLASSSS
>MBI19106_MAGBIO
MVRVIEEFKVTHVSAAPPLVMTMTKGDVVDPYRLRSSGCSPAGRRMGYGMAETSGGAFWTNSPEDRRYGSVGRFSAYIEVKIMDPVTGIAPPPCKQGELWLRGPTITRG
>MBI33573_MAGBIO
MMGKQEHETFVQEEEEYIFQSQFPAVPVPDNLTLPEFVLQEAELFADNVAFVDAVTGKEYTYKQVHHDTRRFAKALRSLGLRKGCVVLVALPNVVEYAVIALGIMSAGGVFSGANPQGLATEIQKQIEDADAKLIVTNGSSYDKVKGLNLPIIVVGDDRVAGAIYWDDLLAAAERAGLDMVDDAVNQNDLCALPYSSGTTGTSKGVMLTHRNLVANLCSSLFSVGPDMVGRFTTLGLMPFFHIYGITGICCATLRNKGKVVVMGRFELRTFLSALITHEVNFAPIVPPIMLALVKNPIVEEFDLQRLKLKAVMTAAAPLAPDLLRAFEEKFPGVQVQEAYGLTEHSCITLSHGDPKQGRGSAKKNSVGFILPNLEIKFIHPDTGRSLPRNSPGELCVRSQCVMLGYYKNKEETERTIDSKGWLHTGDIGYIDDDGDIFIVDRIKELIKYKGFQVAPAELEAILLSHPSIEDAAVFPLPDEEAGEIPAACVVMSRNATESEEDMMKYVASNVATYKRVRVVHFVDKIPKSPSGKIMRRLLRVNMAKRLGTTSHQVAHIG
>MBI17357_MAGBIO
MEKQPQNSDFIYRSKLPDIEIPNHLTLHSYCFENVARFADRPCLINGATGDIYTYADVDRTVRKVAAGLDKLGIEKGHVIMLLLPNCPEFVFAFFGASRRGATTTMANPFYTPNEVSKQAKACDARLIITQACYVDKVRDLAKESDIKIMTIDSPPDGCLHFSELLIADENQIPAVEVSPDDVVALPYSSGTTGLPKGAMLTHKGQVTSVAQQVDGDNPNLYMHSEDVVLCVLPLFHIYSLNSVLLCGLRVGAAILIMQKFEIVALMELVQRYKVTIAAIVPPIMLAIAKSPVVDKYDLSSIRTIMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLSMCLAFAKEPFNIKSGACYLNDPEATKITIDKDGWLHTGDIGLVDNDDELFIVDRLKEIIKYKGFQVAPAELEAMLIAHPKIADAAVVPMKDEAAGEVPVAFVVKSNGSDISEEEIKQYISKQVVFYKRISRVFFVEAIPKAPSGKILRKDLRAKLAAGFPN
>MBI35922_MAGBIO
MDVINDYIYHSKLPDISIPAHLPLHSYCFQNMAHFQSKPCLINGATGEIYTYEEVDLIARKVAAGLHKIGIEQGHVIMLLLPNSPEFVFAFLGASHRGAMATTANPFYTANEISKQARACNPRLIITQACYVEKVRDLAHGNDLKIMCIDSPPEGCLHFSELMEADENNIPAVDISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYFRSDDVLLCVLPLFHIYSLNSVLLCGLRAGASILIMQKFEIVALMELVEKYKITIAPFVPPIVLAIAKSPVVDNYDLSSIRTVMSGAAPMGKELEDAVRAKLPNAILGQGYGMTEAGPVLSMCLAFAKDPFQIKSGSCGTVVRNAELKIVDPETGASLPRNQAGEICIRGAQIMKGYLNDPESTKTTIDKDGWLHTGDIGYLDDDDEIFIVDRLKELIKYKGFQVAPAELEALLIANRDIADAAVVSMKDYLAGEIPVAFVIRSSGSKITEEEVKQYIAKQVIFYKKIHRVFFVDAIPKAPSGKILRKDLRAKLAAGFPN
>MBI37146_MAGBIO
MEKSGYGKDGIYRSLRPRLIIPTDQNLSMVPFLFRNSSSYPQALAIGDAETGESLTFAAFKSTVHNVARGLLRLGIAKGDVVLIFSPNSLYFPLCFFGTVALGAIATTTNPLYTPNELSKQVVDSRPKLIITIAQLWDKVKDLRLPAVIIGSRNHSPPIVSAHKITYFEDLVSGPLPDLPSVPIRQSDTAALFYSSGTTGISKGVILTHRNFISAGLMVTADQENDGEGRNTFLCFVPMFHILGMSIITYAQLQRGNSVVTMGRFDMEAVLRAVEKYRVTYLLVVPPVMIAFAKHSTAWKYDLSSLKRLGSGGAPLVKDIMEECANKLPQALVFQGYGLTESCGIVSTENPKGGIRHYGSTGTLVPGVQSKIVSVDTSKPLPPNQLGEIWVRGPNMMQGRLLNQHICNSLNL
>MBI11856_MAGBIO
MARGATHSSASFPMFHILGMSIITYAQLQRGNSVVTMGRFDMEAVLRAVEKYRVTYLLVVPPVMIAFAKHSTAWKYDLSSLKRLGSGGAPLVKDIMEECANKLPQALVFQGYGLTESCGIVSTENPKGGIRHYGSTGTLVPGVQSKIVSVDTSKPLPPNQLGEIWVRGPNMMQGYFNNPQATELTVDKQGWVHTGDLGYFDDEGRLYVVDRIKELIKYKGYQVAPAELEGLLLSHPEILDAVVIPFPDAEAGEVPIAYVVRSPKSTLTEEHVKEFIAKQVAPFKKLRRVTFVNSVLKSVAGKILRRELIEKVRSKL
>MBI38498_MAGBIO
MFPKECDKLSNTNDVSCAIIDTCLYPKGVIRYAILIFQTLGGSNTNVFKGYFNNPQATELTVDKQGWVHTGDLGYFDDEGRLYVVDRIKELIKYKGYQVAPAELEGLLLSHPEILDAVVIPFPEAEAGEVPIAYVVRSPKSTLTEEHVKEFIAKQVAPFKKLRRVTFVNSVLKSVAGKILRRELIEKVRSKL
>MBI05884_MAGBIO
MEKSGYGKDGIYRSLRSRLIIPTDQNLSMVPFLFRNSSSYPQALAIADAETGESLTFAAFKSTVHNVARGLLRLGIAKGDVVLIFSPNSLYFPLCFLGTVALGAIATTTNPLYTPNELSKQVVDSRPKLIITIPQLWDKVKDLRLPAVIIGSRNHSPPIVSAHKITYFEDLVSGPLPDLPSVPIRQSDTAALFYSSGTTGISKGVILTHRNFISAGLMVTADQENDGEGRNTFLCFVPMFHILGMSIITYAQLQRGNSVVTMGRFDMEAVLRAVEKYRVTYLLAVPPVMIAFAKHSTTWKYDLSSLKRLGSGGAPLGKDIMEECAKKLPQALVFQLTSWR
MLSVASPDTQPPTPAPEGPPPETFIFRSKLPDISLSNHLPLHTYCFEKASEFPDRPCLITGSNGRIYSYAEAHLICQKTAAGLSKLGIKKGDVIMILLQNSPEFVFSFMGASIIGAVTTAANPFCTAAEIYKQFSSSAAKLVITQSQYVNKLRDAGEKFPTIGDELPVITIDDPPENCLHFSVISSADEREVPSVSIEPDDPVALPFSSGTTGLPKGVILTHKSLISSVAQQIDGENPNLHLTTEDVVLCVLPLFHIFSLNSVLLCSLRAASGVLLMQKFEIGTLLDLIQRYRVSVAMVVPPLVLALAKNPTVENFDLSSIRIILSGAAPLGKELEDALRSRVPQALLGQGYGMTEAGPVLSMCLGFAKQPFPTKSGSCGTVVRNADLKVIDPETGFSLQHNQPGEICIRGPQVMKGYLNDPKATASTIDAEGWLHTGDIGYVDDDEEVYIVDRVKELIKFKGFQVPPAELEALLVSHPSIADAAVVPQKDVAAGEVPVAFIVQSNGYDLTEDAVKEFIAKQVVFYKRLHKVYFVHAIPKSPSGKILRKDLRAKLASSSS
>MBI19106_MAGBIO
MVRVIEEFKVTHVSAAPPLVMTMTKGDVVDPYRLRSSGCSPAGRRMGYGMAETSGGAFWTNSPEDRRYGSVGRFSAYIEVKIMDPVTGIAPPPCKQGELWLRGPTITRG
>MBI33573_MAGBIO
MMGKQEHETFVQEEEEYIFQSQFPAVPVPDNLTLPEFVLQEAELFADNVAFVDAVTGKEYTYKQVHHDTRRFAKALRSLGLRKGCVVLVALPNVVEYAVIALGIMSAGGVFSGANPQGLATEIQKQIEDADAKLIVTNGSSYDKVKGLNLPIIVVGDDRVAGAIYWDDLLAAAERAGLDMVDDAVNQNDLCALPYSSGTTGTSKGVMLTHRNLVANLCSSLFSVGPDMVGRFTTLGLMPFFHIYGITGICCATLRNKGKVVVMGRFELRTFLSALITHEVNFAPIVPPIMLALVKNPIVEEFDLQRLKLKAVMTAAAPLAPDLLRAFEEKFPGVQVQEAYGLTEHSCITLSHGDPKQGRGSAKKNSVGFILPNLEIKFIHPDTGRSLPRNSPGELCVRSQCVMLGYYKNKEETERTIDSKGWLHTGDIGYIDDDGDIFIVDRIKELIKYKGFQVAPAELEAILLSHPSIEDAAVFPLPDEEAGEIPAACVVMSRNATESEEDMMKYVASNVATYKRVRVVHFVDKIPKSPSGKIMRRLLRVNMAKRLGTTSHQVAHIG
>MBI17357_MAGBIO
MEKQPQNSDFIYRSKLPDIEIPNHLTLHSYCFENVARFADRPCLINGATGDIYTYADVDRTVRKVAAGLDKLGIEKGHVIMLLLPNCPEFVFAFFGASRRGATTTMANPFYTPNEVSKQAKACDARLIITQACYVDKVRDLAKESDIKIMTIDSPPDGCLHFSELLIADENQIPAVEVSPDDVVALPYSSGTTGLPKGAMLTHKGQVTSVAQQVDGDNPNLYMHSEDVVLCVLPLFHIYSLNSVLLCGLRVGAAILIMQKFEIVALMELVQRYKVTIAAIVPPIMLAIAKSPVVDKYDLSSIRTIMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLSMCLAFAKEPFNIKSGACYLNDPEATKITIDKDGWLHTGDIGLVDNDDELFIVDRLKEIIKYKGFQVAPAELEAMLIAHPKIADAAVVPMKDEAAGEVPVAFVVKSNGSDISEEEIKQYISKQVVFYKRISRVFFVEAIPKAPSGKILRKDLRAKLAAGFPN
>MBI35922_MAGBIO
MDVINDYIYHSKLPDISIPAHLPLHSYCFQNMAHFQSKPCLINGATGEIYTYEEVDLIARKVAAGLHKIGIEQGHVIMLLLPNSPEFVFAFLGASHRGAMATTANPFYTANEISKQARACNPRLIITQACYVEKVRDLAHGNDLKIMCIDSPPEGCLHFSELMEADENNIPAVDISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYFRSDDVLLCVLPLFHIYSLNSVLLCGLRAGASILIMQKFEIVALMELVEKYKITIAPFVPPIVLAIAKSPVVDNYDLSSIRTVMSGAAPMGKELEDAVRAKLPNAILGQGYGMTEAGPVLSMCLAFAKDPFQIKSGSCGTVVRNAELKIVDPETGASLPRNQAGEICIRGAQIMKGYLNDPESTKTTIDKDGWLHTGDIGYLDDDDEIFIVDRLKELIKYKGFQVAPAELEALLIANRDIADAAVVSMKDYLAGEIPVAFVIRSSGSKITEEEVKQYIAKQVIFYKKIHRVFFVDAIPKAPSGKILRKDLRAKLAAGFPN
>MBI37146_MAGBIO
MEKSGYGKDGIYRSLRPRLIIPTDQNLSMVPFLFRNSSSYPQALAIGDAETGESLTFAAFKSTVHNVARGLLRLGIAKGDVVLIFSPNSLYFPLCFFGTVALGAIATTTNPLYTPNELSKQVVDSRPKLIITIAQLWDKVKDLRLPAVIIGSRNHSPPIVSAHKITYFEDLVSGPLPDLPSVPIRQSDTAALFYSSGTTGISKGVILTHRNFISAGLMVTADQENDGEGRNTFLCFVPMFHILGMSIITYAQLQRGNSVVTMGRFDMEAVLRAVEKYRVTYLLVVPPVMIAFAKHSTAWKYDLSSLKRLGSGGAPLVKDIMEECANKLPQALVFQGYGLTESCGIVSTENPKGGIRHYGSTGTLVPGVQSKIVSVDTSKPLPPNQLGEIWVRGPNMMQGRLLNQHICNSLNL
>MBI11856_MAGBIO
MARGATHSSASFPMFHILGMSIITYAQLQRGNSVVTMGRFDMEAVLRAVEKYRVTYLLVVPPVMIAFAKHSTAWKYDLSSLKRLGSGGAPLVKDIMEECANKLPQALVFQGYGLTESCGIVSTENPKGGIRHYGSTGTLVPGVQSKIVSVDTSKPLPPNQLGEIWVRGPNMMQGYFNNPQATELTVDKQGWVHTGDLGYFDDEGRLYVVDRIKELIKYKGYQVAPAELEGLLLSHPEILDAVVIPFPDAEAGEVPIAYVVRSPKSTLTEEHVKEFIAKQVAPFKKLRRVTFVNSVLKSVAGKILRRELIEKVRSKL
>MBI38498_MAGBIO
MFPKECDKLSNTNDVSCAIIDTCLYPKGVIRYAILIFQTLGGSNTNVFKGYFNNPQATELTVDKQGWVHTGDLGYFDDEGRLYVVDRIKELIKYKGYQVAPAELEGLLLSHPEILDAVVIPFPEAEAGEVPIAYVVRSPKSTLTEEHVKEFIAKQVAPFKKLRRVTFVNSVLKSVAGKILRRELIEKVRSKL
>MBI05884_MAGBIO
MEKSGYGKDGIYRSLRSRLIIPTDQNLSMVPFLFRNSSSYPQALAIADAETGESLTFAAFKSTVHNVARGLLRLGIAKGDVVLIFSPNSLYFPLCFLGTVALGAIATTTNPLYTPNELSKQVVDSRPKLIITIPQLWDKVKDLRLPAVIIGSRNHSPPIVSAHKITYFEDLVSGPLPDLPSVPIRQSDTAALFYSSGTTGISKGVILTHRNFISAGLMVTADQENDGEGRNTFLCFVPMFHILGMSIITYAQLQRGNSVVTMGRFDMEAVLRAVEKYRVTYLLAVPPVMIAFAKHSTTWKYDLSSLKRLGSGGAPLGKDIMEECAKKLPQALVFQLTSWR
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University