Enzyme
This plant enzyme, involved in the biosynthesis of anthocyanidins, is known to act on (+)-dihydrokaempferol, (+)-taxifolin, and (+)-dihydromyricetin, although some enzymes may act only on a subset of these compounds. Each dihydroflavonol is reduced to the corresponding cis-flavan-3,4-diol. NAD+ can act instead of NADP+, but more slowly.
DFR
Enzyme
Dihydroflavonol 4-reductase
Enzyme name
EC:1.1.1.219
Entry
Oxidoreductases;Acting on the CH-OH group of donors;With NAD+ or NADP+ as acceptor
Class
(2R,3S,4S)-leucoanthocyanidin:NADP+ 4-oxidoreductase
Sysname
a (2R,3S,4S)-leucoanthocyanidin + NADP+ = a (2R,3R)-dihydroflavonol + NADPH + H+ [RN:R11500]
Reaction
(2R,3R)-dihydroflavonol [CPD:C21417];NADPH [CPD:C00005];H+ [CPD:C00080]
Product
Species information
Lotus japonicus
Species name
Lotus japonicus is a species of eudicot in the family Fabaceae (pea family).
Species info
Gene sequence info
Lotus_japonicus.DFR.fa
Download
>Lj3g0006272.1.Lj1.0v1
MEEMEEAVKKKNRGQVAPSVKYCVTGATGYIGSWLVEALLQRGYSVHATVRDPAKSLHLLSLWKGGEQLRLFQTDLQEVGSFDEAVKGCVGVFCAAASMEFNVTEKENTEAFVQTNIIDPAIKGTINLLKSCLKSNSVKRVIFTSSISTITAKESNGKWKPFVDECCQIQTDNVWNTKASGWVYALSKLLSEEAAFKFAKENNIDLVSVITSTVAGPFFTANIPSSVKVLLSPLTGETEYFKILSAVNARMGSISLVHIEDICSAHIFLMEHAKAEGRYICNSQSCTLSNLATLLSKEYSSSNKQRKSEKHCEKDNVPCEVSSKKLKDLGFSYKHGLDDIIHQTILSCLDYGYLPPV
>Lj4g0019140.1.Lj1.0v1
MSLQPPFLCKFRPFSPISRFSYCSNVTDFPVPAAFDVVSLHNNSNGKWKPFVDECCQIQTDNVCNTKASGWVYALSKLLTEEATFKFAKENDIDITSIVAGPFFTANVPSSVQVLLSPFTFIKLLPSCSLLSNDKLLLTTDQVKISEILELHNSLSSCLHSFIFTVLYCRSSLRILNLCIESVLENSRSSL
>Lj5g0004388.1.Lj1.0v1
MGSAAKTVCVTGSTGFIGSWLVMRLMERGYMVRATVQRDPENMKKVKHLLELPGAKTNLTIWNADLTEEGSFDEAINGCSGVFHLATPMDFNSKDPENEVIKPTINGVLDIMKACQKAKTVRRLVFTSSAGTLDAVEHQKQMFDESCWSDVEFCRRVKMTGWMYFVSKTLAEQEAWKFAKEHDIDFITTIPSLVVGSFLMPTMPPSLITALSPITGNEAHYSIIKQGQFVHLDDLCLAHIFLFEHPESEGRYILSASEATIHDIAKLINSKYPEYNVPTKFSSKKIKEMGFEFKYSLEDMYTGAIDTCKEKGLLLKAAENPSNGITEK
>Lj5g0013235.1.Lj1.0v1
MGSVPETVCVTGAAGFIGSWLVMRLMERGYMVRATVRDPANMKKVKHLLELPEAKTKLTLWKADLAEEGSFDEAIKGCTGVFHVATPMDFESKDPENEVIKPTINGVLDIMKACQKAKTVRRLVFTSSAGTLNVIEHQKQMFDESCWSDVEFCRRVKMTGWMYFVSKTLAEQEAWKFAKEHGIDFITIIPPLVVGSFLMPTMPPSLITALSPITGNEAHYSIIKQGQYVHLDDLCLAHIFLFEHPESEGRYICSASEATIHDIAKLINSKYPEYNIPTKFKNIPDELELVRFSSKKIKDMGFEFKYSLEDMYTGAIDTCKEKGLLPKAAENPSNGK
>Lj5g0004163.1.Lj1.0v1
MGSAAKTVCVTGSTGFIGSWLVMRLMEGGYTVRATVQRDPDNMKKVKHLLELPGAKTNLTIWNADLTEEGSFDEAINGCSGVFHVASPMDFNSKDPENEVIKPSINGVLDIMKACQKAKTVRRLVFTSSAGTLNAVEHQKQMCDESCWSDVEFCRRVKMTGWMYFVSKTLAEQEAWKFAQEHDIDFITIIPSLVVGSFLMPTLPPSLTTALSPITGNEAHYSIIKQGQYVHLDDLCLAHIFLFEHPKSEGRYICSASEATIHDIAKLINSKYPEYNVPTKFKNIPDELELVRFSSKKIKDMGFEFKYSLEDMYTGAIDTCKEKGLLPKAAENPSNGITEK
>Lj5g0010967.2.Lj1.0v1
MGSAAKTVCVTGSTGFIGSWLVMRLMERGYMVRATVQRDPDNMKKVKHLLELPGAKTNLTIWNADLTEEGSFDEAIKGCSGVFHVASPMDFNSKDPENEVIKPAINGVLDIMKACLKAKTVRRLVFTSSAGILSVSERHKHMLDETCWGDLEFCKKVKMTGWMYFVSKELAEQEALKFAKENNIDFVSIIPSLVVGPFLMPTMPPSLYTALCPITGNEAHYMIMKQSQFVHVDDLCLAHIFLFEHPESEGRYMCSACDANIHDIAKLINTKYPEYNVPTKFKNIPDELELVRFSSKKIKDMGFQFKYTLEDMYTGAIDACREKGLLPKAAETPSNGIMEK
>Lj5g0017190.1.Lj1.0v1
MSSESETVCVTGAAGFIGSWLVMRLIERGYTVRATVRDPANMKKVKHLLELPDAKTKLSLWKADLAEEGSFDEAIRGCTGVFHVATPMDFESKDPENEVIKPTINGLLDILKACEKAKTVRRLVFTSSAGTVDVTEHPKPVIDETCWSDIEFCLRVKMTGWMYFVSKTRAEQEAWKYAKEHNIDFVSVIPPLVVGPFLMPTMPPSLITALSLITGNEAHYSIIKQGQYVHLDDLCLAHIFLFENPKAQGRYMCSAYEATIHEVARMINKKYPEFNVPTKFKDIPDELDIIKFSSKKITDLGFKFKYSLEDMYTGAVETCREKGLLPKTAETPATNGTTQK
>Lj6g0023947.1.Lj1.0v1
MKACLKAKIVRRLVFTSSAGTLNVSKQQKHVLDETCWSDVEFCQRVKMTAWMYFASKTLVEQEAWKFAKENNIDLITIIPPLVVGPFLTPTMPPSLTTALSPITGNETHYSIIKQGQFVHIDDLCLAHIFLFEHPEGEGRYVCSACDANIHDIANLINSKYPEYKVPTKFKNIPDQLELVRFSSKKIKDIGFQFKYSLEDMYIAGIDMCREKGLLPKHAKTPGNGITQK
MEEMEEAVKKKNRGQVAPSVKYCVTGATGYIGSWLVEALLQRGYSVHATVRDPAKSLHLLSLWKGGEQLRLFQTDLQEVGSFDEAVKGCVGVFCAAASMEFNVTEKENTEAFVQTNIIDPAIKGTINLLKSCLKSNSVKRVIFTSSISTITAKESNGKWKPFVDECCQIQTDNVWNTKASGWVYALSKLLSEEAAFKFAKENNIDLVSVITSTVAGPFFTANIPSSVKVLLSPLTGETEYFKILSAVNARMGSISLVHIEDICSAHIFLMEHAKAEGRYICNSQSCTLSNLATLLSKEYSSSNKQRKSEKHCEKDNVPCEVSSKKLKDLGFSYKHGLDDIIHQTILSCLDYGYLPPV
>Lj4g0019140.1.Lj1.0v1
MSLQPPFLCKFRPFSPISRFSYCSNVTDFPVPAAFDVVSLHNNSNGKWKPFVDECCQIQTDNVCNTKASGWVYALSKLLTEEATFKFAKENDIDITSIVAGPFFTANVPSSVQVLLSPFTFIKLLPSCSLLSNDKLLLTTDQVKISEILELHNSLSSCLHSFIFTVLYCRSSLRILNLCIESVLENSRSSL
>Lj5g0004388.1.Lj1.0v1
MGSAAKTVCVTGSTGFIGSWLVMRLMERGYMVRATVQRDPENMKKVKHLLELPGAKTNLTIWNADLTEEGSFDEAINGCSGVFHLATPMDFNSKDPENEVIKPTINGVLDIMKACQKAKTVRRLVFTSSAGTLDAVEHQKQMFDESCWSDVEFCRRVKMTGWMYFVSKTLAEQEAWKFAKEHDIDFITTIPSLVVGSFLMPTMPPSLITALSPITGNEAHYSIIKQGQFVHLDDLCLAHIFLFEHPESEGRYILSASEATIHDIAKLINSKYPEYNVPTKFSSKKIKEMGFEFKYSLEDMYTGAIDTCKEKGLLLKAAENPSNGITEK
>Lj5g0013235.1.Lj1.0v1
MGSVPETVCVTGAAGFIGSWLVMRLMERGYMVRATVRDPANMKKVKHLLELPEAKTKLTLWKADLAEEGSFDEAIKGCTGVFHVATPMDFESKDPENEVIKPTINGVLDIMKACQKAKTVRRLVFTSSAGTLNVIEHQKQMFDESCWSDVEFCRRVKMTGWMYFVSKTLAEQEAWKFAKEHGIDFITIIPPLVVGSFLMPTMPPSLITALSPITGNEAHYSIIKQGQYVHLDDLCLAHIFLFEHPESEGRYICSASEATIHDIAKLINSKYPEYNIPTKFKNIPDELELVRFSSKKIKDMGFEFKYSLEDMYTGAIDTCKEKGLLPKAAENPSNGK
>Lj5g0004163.1.Lj1.0v1
MGSAAKTVCVTGSTGFIGSWLVMRLMEGGYTVRATVQRDPDNMKKVKHLLELPGAKTNLTIWNADLTEEGSFDEAINGCSGVFHVASPMDFNSKDPENEVIKPSINGVLDIMKACQKAKTVRRLVFTSSAGTLNAVEHQKQMCDESCWSDVEFCRRVKMTGWMYFVSKTLAEQEAWKFAQEHDIDFITIIPSLVVGSFLMPTLPPSLTTALSPITGNEAHYSIIKQGQYVHLDDLCLAHIFLFEHPKSEGRYICSASEATIHDIAKLINSKYPEYNVPTKFKNIPDELELVRFSSKKIKDMGFEFKYSLEDMYTGAIDTCKEKGLLPKAAENPSNGITEK
>Lj5g0010967.2.Lj1.0v1
MGSAAKTVCVTGSTGFIGSWLVMRLMERGYMVRATVQRDPDNMKKVKHLLELPGAKTNLTIWNADLTEEGSFDEAIKGCSGVFHVASPMDFNSKDPENEVIKPAINGVLDIMKACLKAKTVRRLVFTSSAGILSVSERHKHMLDETCWGDLEFCKKVKMTGWMYFVSKELAEQEALKFAKENNIDFVSIIPSLVVGPFLMPTMPPSLYTALCPITGNEAHYMIMKQSQFVHVDDLCLAHIFLFEHPESEGRYMCSACDANIHDIAKLINTKYPEYNVPTKFKNIPDELELVRFSSKKIKDMGFQFKYTLEDMYTGAIDACREKGLLPKAAETPSNGIMEK
>Lj5g0017190.1.Lj1.0v1
MSSESETVCVTGAAGFIGSWLVMRLIERGYTVRATVRDPANMKKVKHLLELPDAKTKLSLWKADLAEEGSFDEAIRGCTGVFHVATPMDFESKDPENEVIKPTINGLLDILKACEKAKTVRRLVFTSSAGTVDVTEHPKPVIDETCWSDIEFCLRVKMTGWMYFVSKTRAEQEAWKYAKEHNIDFVSVIPPLVVGPFLMPTMPPSLITALSLITGNEAHYSIIKQGQYVHLDDLCLAHIFLFENPKAQGRYMCSAYEATIHEVARMINKKYPEFNVPTKFKDIPDELDIIKFSSKKITDLGFKFKYSLEDMYTGAVETCREKGLLPKTAETPATNGTTQK
>Lj6g0023947.1.Lj1.0v1
MKACLKAKIVRRLVFTSSAGTLNVSKQQKHVLDETCWSDVEFCQRVKMTAWMYFASKTLVEQEAWKFAKENNIDLITIIPPLVVGPFLTPTMPPSLTTALSPITGNETHYSIIKQGQFVHIDDLCLAHIFLFEHPEGEGRYVCSACDANIHDIANLINSKYPEYKVPTKFKNIPDQLELVRFSSKKIKDIGFQFKYSLEDMYIAGIDMCREKGLLPKHAKTPGNGITQK
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University