Enzyme
The enzyme catalyses the biosynthesis of pterostilbene from resveratrol.
ROMT
Enzyme
Trans-resveratrol di-O-methyltransferase
Enzyme name
EC:2.1.1.240
Entry
Transferases;Transferring one-carbon groups;Methyltransferases
Class
S-adenosyl-L-methionine:trans-resveratrol 3,5-O-dimethyltransferase
Sysname
2 S-adenosyl-L-methionine + trans-resveratrol = 2 S-adenosyl-L-homocysteine + pterostilbene (overall reaction) [RN:R09872];(1a) S-adenosyl-L-methionine + trans-resveratrol = S-adenosyl-L-homocysteine + 3-methoxy-4',5-dihydroxy-trans-stilbene [RN:R09870];(1b) S-adenosyl-L-methionine + 3-methoxy-4',5-dihydroxy-trans-stilbene = S-adenosyl-L-homocysteine + pterostilbene [RN:R09871]
Reaction
S-adenosyl-L-homocysteine [CPD:C00021];pterostilbene [CPD:C10287];3-methoxy-4',5-dihydroxy-trans-stilbene [CPD:C20154]
Product
Species information
Lotus japonicus
Species name
Lotus japonicus is a species of eudicot in the family Fabaceae (pea family).
Species info
Gene sequence info
Lotus_japonicus.ROMT.fa
Download
>Lj3g0004260.1.Lj1.0v1
MEFCGDVNATKLFQAQSHVFNHIFSFINSLSLKCAIDLCIPDIIHNYAQPMPLSKLIASLPIHPSKTNSIHRLMRILTHSGFFTQQNVTENELEVRYELTDASRLLIKQNHPFNMTSYMNIVLDPILVEPWYQLSAWFKNEDPTPFYTKHGMSLWEYAVHNPKLGHRFNDAMASETGLTSSAIMEKCKGVFKGLESLVDVGGGIGTMAKAIAKSFPQLQCIVFDLPHVVADLQGSENIKYVGGDMFEAIPPTDSILLKGVLHDWNDEECLKILKKCKEAISRKGKKGKVIIIDVVIRNEKEDSESFETKLFYDLAMMTLVDGKERNEKEWAKLFFSVGFSDYKISPALGFKSLIEVYP
>Lj3g0025279.1.Lj1.0v1
MEFNHEDNATKLFQAQSHVFNHIFSFINSMSLKCAIDLCIPDIIHNYVQPMPLSKLIASLPIHPSKTKSIFRLMRILTNSGFFIQHNVTENELEVRYELTDASRLLIKDHPFSMSSSLHCILDPILTEPWYQFSTWFKNEDPTPFYTKNGMSFWEYFSHNPKHRHRFNDAMASDTRLVSSAVMEKCKGVFKGLETLVDVGGGTGTFAKAIAKSFPQLECIVFDLPHVVSGLQGSENIKYVGGNMFEAIPATDSILLKWILHDWNDEECMKILKNCKEAISKKGKRGKVIIIDVVIGNEKGDSESIETKLFYDLEMMALLGGKERDEKEWAKLIFSVGFSNYKITPVLGLRSLIEVYP
>Lj3g0006231.1.Lj1.0v1
MVFNGEDSGAKLFQAQSHVFNHIYSFINSMSLKCAVELCIPDIIHDYAQPMPLSQLIASLPIHPSKTSGIYRLMQNLTNLGFFTQLNVSENQHEVSYELTDASRLLLKDHPFNMTSFLNITLDPILAKPWYQLSTWFKNDDPTPFYTKHGMSLWEYAAYNPKLNHLFNDAMASSDMQLVSSVVMEKCNGVFHGLETLVDVGGGTGNFVKAIAKSFPQLECIVFDLPHVVAGLQGSENIKYVGGNMFEATPPANAILLKWILHDWNDEKCLKILKNCKEALSREGKLIIIDVVIENEKGDSNFFETKLFYNLDMMTLFDGKERNEKDLAELFFSVGFSNFKITPVLGFRSLIEVYP
>Lj1g0005914.1.Lj1.0v1
MEFQNGEHAAKLLQAQSHIWNHILSFINSMTLKCVVDFVIPDLIHNYGKPIPLSQLIASLPIHPSKTCCVPRLMQIMIHSGFFSQHNVTENELEVEYMLTDTSILLLKDHPFSVAPFLHAMLDPVLKSPWHQFSTWFKNDDRTPFETTLGMIIWEYADRHPKLNKLFNDGMASDSRLITSVIVEKCKGMFDGLKSLVDVGGGLEVQARRRRPLPNHSRI
>Lj1g0020610.1.Lj1.0v1
MELQNGEHAAKLLKAQSHIWNHIFSFINSMSLKCVVDLGIPDLIHNYGKPMPLSQLIVSLPIHPSKTRCIHRLMRMMIHSGFFSQCDDTENELEVEYMLTDASFLLLKDHPFSLTPFLHAMLDPIMTNPWHQFSTWFKNDDPTPFATTHGMMFWNYAGRDPKLNMLFNCGMACDARLITNVVIEKYKGMFDGLESLIDVGGGTGTVAKAIAESFPHLKCIVFDLLHVVVGLQGTENLKYVEGDMFKKIPPADAILLKWILHDWSDEECLTILKKCKEALKSKGKEGKEGDYHRYMVREEEKGDNESVETQLFFDMLMMALVTGKERNKKEWDTLISCAGFSHYKITPVLGLRSVIEIYP
>LjContig00141g0021943.1.Lj1.0v1
MSLKCVVELGIPDIIQNYGKPMPLSQLIASLTIHPSKSCCIHRLMRIMVHSGFFSQHNITENQLEAEYKLTDDSILLLKDHPFSFTRFLHCVFNPIWTESCNHLSTWLKNDDLTPFVTAHGLLPWDFAKRDPKISHLINEAMESDSRLTANVFMEKCKGMLNEFESLVDVGGSTETMAKAIAESFPYLKCTVFDLPYVVADLQGSDNLEYVGGDMFKEILPADAILLKLILHDWNDEVCLQILKNCKEAIKSKGKEGKVIIIDMVMEEEKGDNKSIETQLFFDMVMMVSTTGKERNKKEWVKLITSAGFTDYKITPVLGLRSIIEIYP
>LjContig00141g0003577.1.Lj1.0v1
MEFKDGENAAKLLRAQSHIWNHILSYINSMSLKCVVDLGIPDIIHNYGKPMPLSKLIASLPIHSSKAGNIPRLMRVMIHSGFFSQHNITENELEVEYTLTDDSILLLKGHPFSILPFLLCSNDPILKKPWHHLSTWFKNDDLTPFETAHGMLPWDYANRDPKVNNLLNESMACDAQLIAGVFIEKCKDMLNGLESLVDVGGGTGTMARAIAKSFPRLKCTVFDLPHVVADLQGSENLEYIGGDMFKEIPPADAILLKWILHDWSDEECLKILKNCKEAIKSKGKEGKVIIIDMVIEEDKGDDKSVETQLLFDMGMMVLTTGKERSMKEWGILISSAGFSDYKISPVLGLRSVVEIYP
>LjContig00141g0001851.1.Lj1.0v1
MEIQEGERDAAKLLQALGHIWNHIFSFMNSMTLKCVVELGIPDIIHNFGKPMPLSQLIRSLPIHSSKAGNIHRLMRIMIHSGFFSQHNVSENELEVEYTLTNASMLLLKDHPFSMTPLLHFELDPILTNPWHHLSTWFKNETPTSFETAHGMLFWDYVDRDPKLHRSFNEGMASDSQLITSVFIEKCKDMFNGLESLVDVGGGTGTMTKAIAESFPQLKCTVLDLPHVVSGLQGSENLNYVGGDMFKEIPPTDAILLKLWILHDWSDDECLKILKKCKEAIKSKGKEGKVIIIDMVMEEKKEDHKLVETQLFFDMLMMVVLTGKERNKKEWVKLITSAGFNDYKITQVLGLRSIIEIYP
>LjContig00141g0016231.1.Lj1.0v1
MEFQNGDHASAKLLRSQGHIWNHIFSFINSMSLKYVVELGIPDIIHNYGKPMTLSKLIASLPINPSKTSCIHRLMRIMVHSGFFSRHNVTENELEVEYTLTDDSILLLKDHPFSVIPFLHAMYDPILTKPWHHLSTWFKNDDSTPFATANGMMIWDYADRNPKMNKSFNEGMASDSQLITSVFIEKCKDMFNGSESLVDVAGGTGTLAKAIVESFPHLQCTVFDLPHVVAGLQGSCNLKYVGGDMFKEIPPADVILMKWIMHDWSDEECVKILKKCKEAIKSKGKEGKVIIIDMVIEEENGNDETVETELFLDMVVMALYNAKERNKKEWVKLISSAGFSDYKISPVLGLRSVIEIYP
>LjContig00141g0021303.1.Lj1.0v1
MEIQNDEHATKLLRSQGKIWNHIFSFINSMCLKCVVELGIADIIHNYGKPMPLSQLIAALAVQSSKAGNINRLMRVMVHSGFFSRHNVTENELEVEYSLTDASILLLKDHPFSVTPFLQFELEPISTKTWHHMSNWFKSSDDLNTFETAHGMLPWDYAKRDPNFSNSFHEGMASDARLITSVFMEKCKGMLNEFESLVDVGGGTGTLAKAIAESFPHLKCTVLDLPRIVAGLQGSENLKYVGGDMFKEIPPADAIILKWILHDWSDEDCLKILKKCKEAIKSTGKEGKVIIIDMVMGEENGDHKSVETQLYFDMLMMVALTGKERNKKEWAKLITSAGFSDYKITPVLGLRSVIEIYP
>LjContig00141g0017803.1.Lj1.0v1
MEIQDGEHAAKLFINSMTLKCVVELAIPDIIHNYGKPMPLSQLIASLPIHSSKSCYIHRLKRIMVHSGFFSAIPFLHAMIDPIWIESWHQLSTWLKNDDHTLFETTHGMLLWDYANRDPKLNKSFNERMTSDARLVSSVLIERSENLEYVGGDMFEEIPPADAILLKQWILHDWNDEKCLKILKKCKEAIKSKDKEEKVIIIDMVMEEEKGDDEVFETELFFDIQMMVGLISFAGFNDYKITSVLGLRSIIEIYSKYN
MEFCGDVNATKLFQAQSHVFNHIFSFINSLSLKCAIDLCIPDIIHNYAQPMPLSKLIASLPIHPSKTNSIHRLMRILTHSGFFTQQNVTENELEVRYELTDASRLLIKQNHPFNMTSYMNIVLDPILVEPWYQLSAWFKNEDPTPFYTKHGMSLWEYAVHNPKLGHRFNDAMASETGLTSSAIMEKCKGVFKGLESLVDVGGGIGTMAKAIAKSFPQLQCIVFDLPHVVADLQGSENIKYVGGDMFEAIPPTDSILLKGVLHDWNDEECLKILKKCKEAISRKGKKGKVIIIDVVIRNEKEDSESFETKLFYDLAMMTLVDGKERNEKEWAKLFFSVGFSDYKISPALGFKSLIEVYP
>Lj3g0025279.1.Lj1.0v1
MEFNHEDNATKLFQAQSHVFNHIFSFINSMSLKCAIDLCIPDIIHNYVQPMPLSKLIASLPIHPSKTKSIFRLMRILTNSGFFIQHNVTENELEVRYELTDASRLLIKDHPFSMSSSLHCILDPILTEPWYQFSTWFKNEDPTPFYTKNGMSFWEYFSHNPKHRHRFNDAMASDTRLVSSAVMEKCKGVFKGLETLVDVGGGTGTFAKAIAKSFPQLECIVFDLPHVVSGLQGSENIKYVGGNMFEAIPATDSILLKWILHDWNDEECMKILKNCKEAISKKGKRGKVIIIDVVIGNEKGDSESIETKLFYDLEMMALLGGKERDEKEWAKLIFSVGFSNYKITPVLGLRSLIEVYP
>Lj3g0006231.1.Lj1.0v1
MVFNGEDSGAKLFQAQSHVFNHIYSFINSMSLKCAVELCIPDIIHDYAQPMPLSQLIASLPIHPSKTSGIYRLMQNLTNLGFFTQLNVSENQHEVSYELTDASRLLLKDHPFNMTSFLNITLDPILAKPWYQLSTWFKNDDPTPFYTKHGMSLWEYAAYNPKLNHLFNDAMASSDMQLVSSVVMEKCNGVFHGLETLVDVGGGTGNFVKAIAKSFPQLECIVFDLPHVVAGLQGSENIKYVGGNMFEATPPANAILLKWILHDWNDEKCLKILKNCKEALSREGKLIIIDVVIENEKGDSNFFETKLFYNLDMMTLFDGKERNEKDLAELFFSVGFSNFKITPVLGFRSLIEVYP
>Lj1g0005914.1.Lj1.0v1
MEFQNGEHAAKLLQAQSHIWNHILSFINSMTLKCVVDFVIPDLIHNYGKPIPLSQLIASLPIHPSKTCCVPRLMQIMIHSGFFSQHNVTENELEVEYMLTDTSILLLKDHPFSVAPFLHAMLDPVLKSPWHQFSTWFKNDDRTPFETTLGMIIWEYADRHPKLNKLFNDGMASDSRLITSVIVEKCKGMFDGLKSLVDVGGGLEVQARRRRPLPNHSRI
>Lj1g0020610.1.Lj1.0v1
MELQNGEHAAKLLKAQSHIWNHIFSFINSMSLKCVVDLGIPDLIHNYGKPMPLSQLIVSLPIHPSKTRCIHRLMRMMIHSGFFSQCDDTENELEVEYMLTDASFLLLKDHPFSLTPFLHAMLDPIMTNPWHQFSTWFKNDDPTPFATTHGMMFWNYAGRDPKLNMLFNCGMACDARLITNVVIEKYKGMFDGLESLIDVGGGTGTVAKAIAESFPHLKCIVFDLLHVVVGLQGTENLKYVEGDMFKKIPPADAILLKWILHDWSDEECLTILKKCKEALKSKGKEGKEGDYHRYMVREEEKGDNESVETQLFFDMLMMALVTGKERNKKEWDTLISCAGFSHYKITPVLGLRSVIEIYP
>LjContig00141g0021943.1.Lj1.0v1
MSLKCVVELGIPDIIQNYGKPMPLSQLIASLTIHPSKSCCIHRLMRIMVHSGFFSQHNITENQLEAEYKLTDDSILLLKDHPFSFTRFLHCVFNPIWTESCNHLSTWLKNDDLTPFVTAHGLLPWDFAKRDPKISHLINEAMESDSRLTANVFMEKCKGMLNEFESLVDVGGSTETMAKAIAESFPYLKCTVFDLPYVVADLQGSDNLEYVGGDMFKEILPADAILLKLILHDWNDEVCLQILKNCKEAIKSKGKEGKVIIIDMVMEEEKGDNKSIETQLFFDMVMMVSTTGKERNKKEWVKLITSAGFTDYKITPVLGLRSIIEIYP
>LjContig00141g0003577.1.Lj1.0v1
MEFKDGENAAKLLRAQSHIWNHILSYINSMSLKCVVDLGIPDIIHNYGKPMPLSKLIASLPIHSSKAGNIPRLMRVMIHSGFFSQHNITENELEVEYTLTDDSILLLKGHPFSILPFLLCSNDPILKKPWHHLSTWFKNDDLTPFETAHGMLPWDYANRDPKVNNLLNESMACDAQLIAGVFIEKCKDMLNGLESLVDVGGGTGTMARAIAKSFPRLKCTVFDLPHVVADLQGSENLEYIGGDMFKEIPPADAILLKWILHDWSDEECLKILKNCKEAIKSKGKEGKVIIIDMVIEEDKGDDKSVETQLLFDMGMMVLTTGKERSMKEWGILISSAGFSDYKISPVLGLRSVVEIYP
>LjContig00141g0001851.1.Lj1.0v1
MEIQEGERDAAKLLQALGHIWNHIFSFMNSMTLKCVVELGIPDIIHNFGKPMPLSQLIRSLPIHSSKAGNIHRLMRIMIHSGFFSQHNVSENELEVEYTLTNASMLLLKDHPFSMTPLLHFELDPILTNPWHHLSTWFKNETPTSFETAHGMLFWDYVDRDPKLHRSFNEGMASDSQLITSVFIEKCKDMFNGLESLVDVGGGTGTMTKAIAESFPQLKCTVLDLPHVVSGLQGSENLNYVGGDMFKEIPPTDAILLKLWILHDWSDDECLKILKKCKEAIKSKGKEGKVIIIDMVMEEKKEDHKLVETQLFFDMLMMVVLTGKERNKKEWVKLITSAGFNDYKITQVLGLRSIIEIYP
>LjContig00141g0016231.1.Lj1.0v1
MEFQNGDHASAKLLRSQGHIWNHIFSFINSMSLKYVVELGIPDIIHNYGKPMTLSKLIASLPINPSKTSCIHRLMRIMVHSGFFSRHNVTENELEVEYTLTDDSILLLKDHPFSVIPFLHAMYDPILTKPWHHLSTWFKNDDSTPFATANGMMIWDYADRNPKMNKSFNEGMASDSQLITSVFIEKCKDMFNGSESLVDVAGGTGTLAKAIVESFPHLQCTVFDLPHVVAGLQGSCNLKYVGGDMFKEIPPADVILMKWIMHDWSDEECVKILKKCKEAIKSKGKEGKVIIIDMVIEEENGNDETVETELFLDMVVMALYNAKERNKKEWVKLISSAGFSDYKISPVLGLRSVIEIYP
>LjContig00141g0021303.1.Lj1.0v1
MEIQNDEHATKLLRSQGKIWNHIFSFINSMCLKCVVELGIADIIHNYGKPMPLSQLIAALAVQSSKAGNINRLMRVMVHSGFFSRHNVTENELEVEYSLTDASILLLKDHPFSVTPFLQFELEPISTKTWHHMSNWFKSSDDLNTFETAHGMLPWDYAKRDPNFSNSFHEGMASDARLITSVFMEKCKGMLNEFESLVDVGGGTGTLAKAIAESFPHLKCTVLDLPRIVAGLQGSENLKYVGGDMFKEIPPADAIILKWILHDWSDEDCLKILKKCKEAIKSTGKEGKVIIIDMVMGEENGDHKSVETQLYFDMLMMVALTGKERNKKEWAKLITSAGFSDYKITPVLGLRSVIEIYP
>LjContig00141g0017803.1.Lj1.0v1
MEIQDGEHAAKLFINSMTLKCVVELAIPDIIHNYGKPMPLSQLIASLPIHSSKSCYIHRLKRIMVHSGFFSAIPFLHAMIDPIWIESWHQLSTWLKNDDHTLFETTHGMLLWDYANRDPKLNKSFNERMTSDARLVSSVLIERSENLEYVGGDMFEEIPPADAILLKQWILHDWNDEKCLKILKKCKEAIKSKDKEEKVIIIDMVMEEEKGDDEVFETELFFDIQMMVGLISFAGFNDYKITSVLGLRSIIEIYSKYN
Gene tree
Gene tree
Sheng jun & Dong yang Teams, Yunnan agriculture University